Assays

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978 Assays visible to you, out of a total of 1941

P2011.1.2 written in Antimony and converted in SBML using python package Tellurium. Parameters values correspond to P2011.1.2

Andrew's work-in-progress P2012 version. NB KNOWN PROBLEMS do not use lightly. Derived from PLM_49, after removing ABA regulation and tidying up the SBML in COPASI. Please see version comments for IMPORTANT notes.Comments No parameters constrained in version 1 file. 2013-02-26 17:31:26 3 amillar2 andrew.millar@ed.ac.uk Compiled successfully in SBSI for optimisation. 2013-02-26 17:28:18 3 amillar2 andrew.millar@ed.ac.ukVersion Comments Version 1 is file P2012_NoSinkNoABAParamsNom38_freshCopasi.xml ...

Submitter: BioData SynthSys

Biological problem addressed: Gene Regulatory Network

Investigation: Millar, Andrew (ex-PlaSMo models)

Study: P2012_AJMv2_NoABA - PLM_69

Andrew's work-in-progress P2012 version. NB KNOWN PROBLEMS do not use lightly. Derived from PLM_49, after removing ABA regulation and tidying up the SBML in COPASI. Please see version comments for IMPORTANT notes.Comments No parameters constrained in version 1 file. 2013-02-26 17:31:26 3 amillar2 andrew.millar@ed.ac.uk Compiled successfully in SBSI for optimisation. 2013-02-26 17:28:18 3 amillar2 andrew.millar@ed.ac.ukVersion Comments Version 2 is file P2012_fin_NoABAv4.xml of 6th March.

It ...

Submitter: BioData SynthSys

Biological problem addressed: Gene Regulatory Network

Investigation: Millar, Andrew (ex-PlaSMo models)

Study: P2012_AJMv2_NoABA - PLM_69

In silico check and filtering for potential Pan-assay interference compounds.

Cells were grown to mid-exponential phase (OD600nm ~0.2) in GM17 medium at 37°C (with 0.15 mM ZnSO4 where relevant) and 84 ml of culture was mixed by inverting with 8.4 ml of fixing solution (50 mM Tris pH 8.0, 100 mM NaCl, 0.5 mM EGTA, 1 mM EDTA, 30% (v/v) formaldehyde) and incubated at room temperature for 30 min. Cells were disrupted and crosslinked DNA was sheared by sonication. Antibodies coupled to magnetic beads were used to pull down cross-linked complexes. DNA was purified, amplified, ...

This is part of the GreenLab Functional-Structural Plant Model for Arabidopsis published in Christophe et al 2008. This model was re-factored, to facilitate the integration in the Chew et al Framework Model, and it cannot be run as a standalone model.  Related PublicationsAngélique Christophe A E, Véronique Letort B, Irène Hummel A, Paul-Henry Cournède B, Philippe de Reffye C, Jérémie Lecœur (2008). A model-based analysis of the dynamics of carbon balance at the whole-plant level in Arabidopsis ...

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Submitter: Ziva Ramsak

Assay type: Q_PCR

Technology type: qPCR

Investigation: MOA - Multiomics analysis of potato response to...

Study: MOA2010-05

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Kinetic characterisation en mathematical modelling of PFK.

Kinetic characterisation en mathematical modelling of PGI.

Kinetic characterisation en mathematical modelling of PGK.

PGK - GAPDH models

Submitter: Jacky Snoep

Biological problem addressed: Model Analysis Type

Investigation: Phosphoglycerate kinase acts as a futile cycle ...

Study: PGK-GAPDH 30C & 70C

Experimental data for the yeast PGK incubations at 30C, with and without recycling of ATP.

A model for the PGK reaction of yeast in presence or absence of the ATP recycling reactions

Submitter: Jacky Snoep

Biological problem addressed: Model Analysis Type

Investigation: Phosphoglycerate kinase acts as a futile cycle ...

Study: PGK-30C

Changes in metabolite concentrations were either quantified via 31P NMR or enzymatically

PGK 70C model

Submitter: Jacky Snoep

Biological problem addressed: Model Analysis Type

Investigation: Phosphoglycerate kinase acts as a futile cycle ...

Study: PGK-70C

Mathematical model for PGK kinetics, saturation with ADP, ATP, 3PG and BPG.

Submitter: Jacky Snoep

Biological problem addressed: Enzymology

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

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Kinetic characterisation en mathematical modelling of PGM.

Plant material The same plant material used for transcriptome analysis in (Flis et al., 2016) was the basis of our proteome study. Briefly, Arabidopsis thaliana Col-0 plants were grown on GS 90 soil mixed in a ratio 2:1 (v/v) with vermiculite. Plants were grown for 1 week in a 16 h light (250 μmol m−2 s−1, 20 °C)/8 h dark (6 °C) regime followed by an 8 h light (160 μmol m−2 s−1, 20 °C)/16 h dark (16 °C) regime for one week. Plants were then replanted with five seedlings per pot, transferred for ...

L. lactis, S. pyogenes and E. faecalis were grown in C-limited chemostat cultures at various pH's and dilution rates. General flux distribution, yields and other physiological factors were studied.

These files show physiological measurements from the Sheffield Infors chemostat which were made during acetate calibration and also when sampling for the steady-state transcriptional profiles.

Kinetic characterisation en mathematical modelling of PK.

Measurement of plasma parameters: activities of cholinesterase, alanine aminotransferase, aspartate aminotransferase + cortisol and total protein levels in cod exposed to chlorpyrifos-methyl

Submitter: Karina Dale

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: 1 hidden item

Study: In vivo Nord 1: Chlorpyrifos-methyl

To check if all works fine after struts update. Checking editorial options

Additional Attributes
tested:

Yes, against schema



Originally submitted to PLaSMo on 2013-11-22 15:15:40

Submitter: BioData SynthSys

Biological problem addressed: Gene Regulatory Network

Investigation: Zielinski, Tomasz

Study: Plasmo test model1 - PLM_80

The model is an extensio of PLM_67v3 with an additional an additional variable Temp in ODE 25. This change allows to simulated warm pulses that affect EC stability using COPASI. 

Originally submitted to PLaSMo on 2014-03-10 13:16:25

Submitter: BioData SynthSys

Biological problem addressed: Gene Regulatory Network

Investigation: Urquiza Garcia, Uriel

Study: PLM_67v3withTempPulse - PLM_81

The external potassium changes will be monitored by the MIFE and FLISE technique. This allows an estimation of internal potassium changes by determining an initial concentration.

Is internal potassium affected by mutations in the Trk1,2 system? Under which conditions? Potassium measurements in wild type and TRK mutants grown and/or incubated under several external conditions.

The potassium fluxes will be estimated from the internal and external concentration changes.

How potassium starvation regulates the parameters of rubidium (potassium) transport. Analysis of transport characteristics during the starvation process. Kinetic characteristics of rubidium transport.

The potassium influx after the addition of a certain amount of KCl to a potassium free medium, followed by the injection of glucose will be measured by using the MIFE and FLISE technique. This reveals a time course of potassium. Also the external potassium concentrations will be measured.

Genes are transcribed in polysictronic messages (pre-mRNA) that are destined for either maturation into mRNAs, or degradation. Since transcription regulation is non-existent with few exceptions, the rate of pre-mRNA processing, together with mRNA decay and translation rates, are believed to control gene expression. In this assay, 2T1 blood form trypanosomes are subject to treatment by ActinomycinD for 5 minutes, inhibiting transcription. The cells are harvested, depleted for ribosomal RNA, and ...

Prediction of patient urinary acylcarnitine under metabolic decompensation. Generates Fig. 3, Table 1, and Table S2 in the associated publication.

Download and unzip "Model_notebooks.rar" and run "7, Fig3+4+S1+S3-ACADDs-[needs-(1)]-20221109.nb" after running "1, generate-model-20221109.nb"

Submitter: Christoff Odendaal

Biological problem addressed: Model Analysis Type

Investigation: Mitochondrial fatty acid oxidation in human liver

Study: Model analysis

Preparation needed to use Simulation Foundry, Version 1.5.

Please read the manual before working with this Simulation Foundry.

Pay careful attention to the installation instructions.

Note the known issues.

Submitter: Gudrun Gygli

Assay type: Instructions

Technology type: Technical Computer Installation

Investigation: 1 hidden item

Study: Simulation Foundry for Methanol-Water Mixtures

Prepared multimeric tubulin protein receptors for docking studies, generated by alignment of two identical models of alpha-tubulin on alpha-tubulin chains of two neighboring protofilaments.

Conversion of KDX to KGSA by Caulobacter crescentus KDXD, measured in NMR.

Submitter: Jacky Snoep

Biological problem addressed: Model Analysis Type

Investigation: Caulobacter crescentus Weimberg pathway

Study: Progress curves

Conversion of KGSA to KG by Caulobacter crescentus KGSADH, measured in NMR.

Conversion of XA to KDX by Caulobacter crescentus XAD, measured in NMR.

Submitter: Jacky Snoep

Biological problem addressed: Model Analysis Type

Investigation: Caulobacter crescentus Weimberg pathway

Study: Progress curves

Conversion of Xyl to XLAC by Caulobacter crescentus XDH, measured in NMR.

Submitter: Jacky Snoep

Biological problem addressed: Model Analysis Type

Investigation: Caulobacter crescentus Weimberg pathway

Study: Progress curves

Conversion of XLAC to XA by Caulobacter crescentus XLA, measured in NMR.

Submitter: Jacky Snoep

Biological problem addressed: Model Analysis Type

Investigation: Caulobacter crescentus Weimberg pathway

Study: Progress curves

Conversion of XYL to KG by sequential addition of Weimberg pathway enzymes of Caulobacter crescentus, measured in NMR. https://jjj.bio.vu.nl/models/experiments/shen2020_fig2c/simulate

No description specified

Is protein content of trk1,2 mutants affected? Determination of proteins in wild type and TRK mutants

What are the main proteins identified? Spots sampling and identification by MS

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Data derived from protein samples

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Some results from proteomics analyses of cod liver exposed to PAH and PFAS

Submitter: Karina Dale

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: 1 hidden item

Study: PAH/PFAS mixture toxicity in vivo

Protein copy number at 6h, 12h, 24h, 48h, 72h, 96h, average values and SD for the measurements

Submitter: Niels Zondervan

Assay type: Proteomics

Technology type: Technology Type

Investigation: Modelling of M. pneumoniae metabolism

Study: Proteomics analysis

The data is submitted to the PRIDE repository, and will be linked here.

Submitter: Marta Eide

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: 1 hidden item

Study: In vivo II - GW and WY: Effects on cod lipid me...

Examples of proteomics templates for gele electrophoresis data that conform to the MIAPE-GE specification

Some examples of proteomics templates for Mass Spectrometry data that conform to the MIAPE specification

The external pH changes will be monitored by the MIFE and FLISE technique. This allows an estimation of internal pH changes by determining an initial pH. pH changes will be also determined by using green fluorescent protein dyes. Relating the proton efflux and the change of internal pH allows an estimate of the proton buffering capacity.

Related to the internal pH changes the proton efflux will be estimated from the internal and external concentration changes.

In parallel with the potassium influx the efflux of protons is monitored by measuring the external proton concentration changes with MIFE or FLISE.

No description specified

Pyruvate formate-lyase (PFL) is an important enzyme in the metabolic pathway of lactic acid bacteria (LAB) and is held responsible for the regulation of the shift between homolactic acid to mixed acid fermentation. PFL catalysis the reversible reaction of acetyl-CoA and formate into pyruvate and CoA. A glycyl radical, who is regenerated within the reaction, is involved; therefore, PFL works only under strictly anaerobic conditions. For its activation, the C-terminal domain has to bind to the ...

Submitter: Stefan Henrich

Biological problem addressed: Model Analysis Type

Investigation: The Attic

Study: Pyruvate formate-lyase (PFL)

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