Lars Wöhlbrand

Cell biological and proteogenomic study of the chloroplast of the marine, bloom-forming dinoflagellate P. cordatum

Cells of P. cordatum adapted to growth at 20 °C and constant light:dark conditions were shifted to growth at 26 °C and 30 °C, respectively, with similar illumination conditions. During exponential and stationary growth, cells were collected for transcriptomic, proteomic and metabolomic analyses.

Tryptic peptides of P. cordatum were separated by 2D SCX-/RP nanoLC coupled to ESI-Iontrap MS/MS.

The membrane fraction of Prorcentrum cordatum was prepared and proteins solubilized using SDS. Subsequently, proteins were separated by SDS-PAGE, subjected to tryptic in-gel digest and nanoLC ESI-iontrap MS/MS.

Prepared (sub-)cellular fractions were separated by gradient SDS-PAGE and entire sample lanes cut into pieces prior to in-gel digest and nanoLC separation coulpled to iontrap MS/MS detection.

Prorocentrum cordatum was subjected to whole cell shotgun analyses (in-solution digest) unsing nanoLC ESI-intrap MS/MS

Detailed description of the methods applied for proteomic analyses of P. cordatum heat stress response