Assays

250 Assays visible to you, out of a total of 462

PGK - GAPDH models

PGK 70C model

Contributor: Jacky Snoep

Biological problem addressed: Modelling Analysis

Investigation: Phosphoglycerate kinase acts as a futile cycle ...

Study: PGK-70C

BPG stability analysis

Contributor: Jacky Snoep

Biological problem addressed: Modelling Analysis

Investigation: Phosphoglycerate kinase acts as a futile cycle ...

Study: BPG stability

Changes in metabolite concentrations were either quantified via 31P NMR or enzymatically

No description specified
No description specified

BPG degradation at 70C

A model for the PGK reaction of yeast in presence or absence of the ATP recycling reactions

Contributor: Jacky Snoep

Biological problem addressed: Modelling Analysis

Investigation: Phosphoglycerate kinase acts as a futile cycle ...

Study: PGK-30C

Experimental data for the yeast PGK incubations at 30C, with and without recycling of ATP.

No description specified
No description specified

Contributor: Theresa Kouril

Assay type: Experimental Assay

Technology type: Enzymatic activity measurements

Investigation: 1 hidden item

Study: 1 hidden item

Measurements of external metabolites based on growth curve data.
Flux estimates for uptake of external metabolites such as glucose and production rates for external metabolites lactate and acetate

Contributor: Niels Zondervan

Assay type: Experimental Assay

Technology type: Technology type

Investigation: 1 hidden item

Study: Metabolomics measurements

Simulation of double mutants and perturbations and time series samples using for Sample 1 only OE mutants of which we update the enzyme concentrations. For each second mutant the enzyme concentrations in case of OE and KO mutants in updated and the metabolite concentrations of the second sample are loaded in the model.
Using this approach the model approximately predicts combinatorial effects of OE mutations with other mutations, perturbations and time series concentrations.

Contributor: Niels Zondervan

Biological problem addressed: Modelling Analysis

Investigation: 1 hidden item

Study: Core model combinatorial predictions

No description specified

Genome scale metabolic model of Sulfolobus solfataricus
specific scenario: modelling of L-fucose degradation pathways

No description specified

Contributor: Ron Henkel

Biological problem addressed: Cell cycle

Investigation: Hands-on: Model Management in SEEK

Study: Hands-On: Tyson1991 - Cell Cycle 6 var

No description specified

S. pyogenes wildetype, an arcA- and a glnA-deletion mutants were grown in CDM-LAB cultures at pH 6.5 and 7.5 and at a growth rate of 0.05

In order to construct an in vivo-like buffer for S. pyogenes, the intracellular concentrations of Fe, K, Mg, Mn Na, P and S elements were determined via ICP-AES (inductively coupled plasma atomic emissionspectroscopy) method at the Institute of Land Use, University of Rostock. The samples for the analysis were obtained from a steady state culture grown on CDM-LAB with glucose.

Determination of essential amino acids for Streptococcus pyogenes

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