Assays

What is an Assay?

1319 Assays visible to you, out of a total of 2303

RNA-Seq PA14 A549

Pseudomonas aeruginosa and its vesicles

Cells were seeded in 6-well plates and incubated at 37 °C and 5 % CO2 overnight. The next day, cells were washed with 1X PBS, treated with BMVs and incubated at 37 °C and 5 % CO2 for 24 h. RNA isolation was performed by using the NucleoSpin® RNA kit (MACHEREY-NAGEL, 740955.50). cDNA synthesis was performed and libraries were constructed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina at the Genome Analytics at Helmholtz Centre for Infection Research. Libraries were sequenced ...

Submitter: Meina Neumann-Schaal

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: 1 hidden item

Study: RNASeq comparing vesicle-treated to a non-treat...

Organisms: No organisms

SOPs: No SOPs

Data files: RNA-Seq PA14 A549

Snapshots: No snapshots

Created: 11th Aug 2025 at 06:02, Last updated: 12th Aug 2025 at 04:13

Influence of reactor mode on the cofermentation of cellulose and xylan targeting MCC

Cell4Chem

No description specified

Submitter: Juan Iglesias-Riobó

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: 1 hidden item

Study: 1 hidden item

Organisms: No organisms

SOPs: No SOPs

Data files: CelluloseXylan_SBR_1, CelluloseXylan_SBR_1stStage, CelluloseXylan_SBR_2ndStage, CelluloseXylan_SFBR_3, Inoculum_CelluloseXylan_SBR_1, MineralMedium_ CelluloseXylan, Poster_Targeting medium chain carboxylates in t...

Snapshots: Efficient medium-chain carboxylate production from a cellulose:xylan mixture

DOI: 10.15490/fairdomhub.1.assay.2591.1

Created: 24th Mar 2025 at 12:42, Last updated: 8th Aug 2025 at 18:10

Shoulders Lab

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Shoulders Lab Individual Publications

Study: Human cartilage model of the precocious osteoar...

Organisms: No organisms

SOPs: No SOPs

Data files: Collagen_Shoulders_AllMetadata_v2.xlsx

Snapshots: No snapshots

Created: 5th Aug 2025 at 20:36, Last updated: 5th Aug 2025 at 20:42

Short Read Sequencing - Data Linked

Shoulders Lab

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Shoulders Lab Individual Publications

Study: Human cartilage model of the precocious osteoar...

Organisms: No organisms

SOPs: P.SHO-250605-V1_Collagen_RNAseq.docx

Data files: samples-250609-SC-R719C-D.SEQ.xlsx, samples-250609-SC-R719C-DNA.xlsx

Snapshots: No snapshots

Created: 9th Jun 2025 at 14:15, Last updated: 5th Aug 2025 at 20:37

Gene Expression Analysis - Data Linked

Shoulders Lab

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Shoulders Lab Individual Publications

Study: Human cartilage model of the precocious osteoar...

Organisms: No organisms

SOPs: P.SHO-250605-V1_Collagen_RNAseq.docx

Data files: samples-250609-SC-R719C-A.GEX.xlsx, samples-250609-SC-R719C-D.SEQ.xlsx

Snapshots: No snapshots

Created: 9th Jun 2025 at 14:16, Last updated: 5th Aug 2025 at 20:37

Flow Cytometry Analysis - Data Linked

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Intravenous BCG-mediated protection against tub...

Organisms: No organisms

SOPs: No SOPs

Data files: 1 hidden item

Snapshots: No snapshots

Created: 23rd Jan 2025 at 20:40, Last updated: 21st Jul 2025 at 15:41

MetNet

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: MetNet

Study: Chemo-mechanical diffusion waves explain collec...

Organisms: No organisms

SOPs: No SOPs

Data files: AllMetadata VS-POD.xlsx

Snapshots: No snapshots

Created: 16th Jul 2025 at 17:31, Last updated: 16th Jul 2025 at 18:59

Modeling - Data Linked

MetNet

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: MetNet

Study: Chemo-mechanical diffusion waves explain collec...

Organisms: No organisms

SOPs: No SOPs

Data files: samples-publish_250716_A.IMG.xlsx, samples-publish_250716_MDL.xlsx

Snapshots: No snapshots

Created: 16th Jul 2025 at 17:30, Last updated: 16th Jul 2025 at 18:57

Imaging Analysis - Data Linked

MetNet

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: MetNet

Study: Chemo-mechanical diffusion waves explain collec...

Organisms: No organisms

SOPs: No SOPs

Data files: samples-publish_250716_A.IMG.xlsx, samples-publish_250716_D.IMG.xlsx

Snapshots: No snapshots

Created: 16th Jul 2025 at 17:29, Last updated: 16th Jul 2025 at 18:45

Live Cell Imaging - Data Linked

MetNet

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: MetNet

Study: Chemo-mechanical diffusion waves explain collec...

Organisms: No organisms

SOPs: No SOPs

Data files: samples-publish_250716_CEL.xlsx, samples-publish_250716_D.IMG.xlsx

Snapshots: No snapshots

Created: 16th Jul 2025 at 17:28, Last updated: 16th Jul 2025 at 18:09

Dendritic Cell Preparation - Metadata

MetNet

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: MetNet

Study: Chemo-mechanical diffusion waves explain collec...

Organisms: No organisms

SOPs: P.SHE-250716_V1-DC_Preparation.docx

Data files: samples-publish_250716_CEL.xlsx

Snapshots: No snapshots

Created: 16th Jul 2025 at 17:14, Last updated: 16th Jul 2025 at 17:42

Cell Imaging - Data Linked

Shoulders Lab

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Shoulders Lab Individual Publications

Study: Human cartilage model of the precocious osteoar...

Organisms: No organisms

SOPs: P.SHO-250605-V1_Collagen_Imaging.docx

Data files: TUNEL_quantification.xlsx, samples-250609-SC-R719C-CEL.xlsx, samples-250609-SC-R719C-D.IMG.xlsx

Snapshots: No snapshots

Created: 9th Jun 2025 at 14:12, Last updated: 9th Jun 2025 at 15:58

Proteomics Analysis - Data Linked

Shoulders Lab

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Shoulders Lab Individual Publications

Study: Human cartilage model of the precocious osteoar...

Organisms: No organisms

SOPs: P.SHO-250605-V1_Collagen_Proteomics.docx

Data files: samples-250609-SC-R719C-A.MSP.xlsx, samples-250609-SC-R719C-D.MSP.xlsx

Snapshots: No snapshots

Created: 9th Jun 2025 at 14:20, Last updated: 9th Jun 2025 at 15:56

LC - MS/MS (Proteomics) - Data Linked

Shoulders Lab

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Shoulders Lab Individual Publications

Study: Human cartilage model of the precocious osteoar...

Organisms: No organisms

SOPs: P.SHO-250605-V1_Collagen_Proteomics.docx

Data files: samples-250609-SC-R719C-CEL.xlsx, samples-250609-SC-R719C-D.MSP.xlsx

Snapshots: No snapshots

Created: 9th Jun 2025 at 14:17, Last updated: 9th Jun 2025 at 15:49

Library Creation - Metadata

Shoulders Lab

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Shoulders Lab Individual Publications

Study: Human cartilage model of the precocious osteoar...

Organisms: No organisms

SOPs: P.SHO-250605-V1_Collagen_RNAseq.docx

Data files: samples-250609-SC-R719C-DNA.xlsx, samples-250609-SC-R719C-RNA.xlsx

Snapshots: No snapshots

Created: 9th Jun 2025 at 14:14, Last updated: 9th Jun 2025 at 15:27

iPSC Culture and Chondronoid Formation - Metadata

Shoulders Lab

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Shoulders Lab Individual Publications

Study: Human cartilage model of the precocious osteoar...

Organisms: No organisms

SOPs: P.SHO-250605-V1_Collagen_Imaging.docx, P.SHO-250605-V1_Collagen_Proteomics.docx, P.SHO-250605-V1_Collagen_RNAseq.docx, P.SHO-250605-V1_Collagen_iPSC.docx

Data files: samples-250609-SC-R719C-CEL.xlsx

Snapshots: No snapshots

Created: 9th Jun 2025 at 14:11, Last updated: 9th Jun 2025 at 15:00

RNA Extraction - Metadata

Shoulders Lab

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Shoulders Lab Individual Publications

Study: Human cartilage model of the precocious osteoar...

Organisms: No organisms

SOPs: P.SHO-250605-V1_Collagen_RNAseq.docx

Data files: samples-250609-SC-R719C-CEL.xlsx, samples-250609-SC-R719C-RNA.xlsx

Snapshots: No snapshots

Created: 9th Jun 2025 at 14:13, Last updated: 9th Jun 2025 at 14:59

PoLiMeR - Polymers in the Liver: Metabolism and Regulation

(Show All)

MCAD-KO mice (C57BL/6J background) were exposed to three different conditions: "fed", "fasting", and "fasting + cold". In this study we determined the concentration of various CoA biosynthetic intermediates (including CoA) and the expression of CoA metabolic genes in liver tissue samples from these groups of mice.

Submitter: Christoff Odendaal

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Mitochondrial fatty acid oxidation in human liver

Study: Sequestration of CoA and adaptation of CoA meta...

Organisms: No organisms

SOPs: No SOPs

Data files: Mice CoA biosynthetic intermediates (Fig. 4C), Mice qPCR (Fig. 4E-F)

Snapshots: No snapshots

Created: 9th May 2025 at 15:22, Last updated: 16th May 2025 at 11:45

Computational model (in silico)

PoLiMeR - Polymers in the Liver: Metabolism and Regulation

(Show All)

No description specified

Submitter: Christoff Odendaal

Biological problem addressed: Model Analysis Type

Investigation: Mitochondrial fatty acid oxidation in human liver

Study: Sequestration of CoA and adaptation of CoA meta...

Organisms: No organisms

Models: Model notebooks, odendaal4

SOPs: No SOPs

Data files: No Data files

Snapshots: No snapshots

Created: 9th May 2025 at 14:40, Last updated: 16th May 2025 at 11:45

HepG2 cells (in vitro)

PoLiMeR - Polymers in the Liver: Metabolism and Regulation

(Show All)

The behaviour of a published MCAD-KO HepG2 cell line was compared to that of a wild-type HepG2 cell line in terms of the changes in acyl-CoA and acylcarnitine levels (measured by HILIC-MS/MS) and gene expression pertaining to CoA metabolism (characterised by qPCR). Additionally, the incorporation of label from stable isotope-labelled pantothenate (vitamine B5) was over 24 hours of exposure to an energetic stressor was also investigated.

Submitter: Christoff Odendaal

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Mitochondrial fatty acid oxidation in human liver

Study: Sequestration of CoA and adaptation of CoA meta...

Organisms: Homo sapiens (batch)

SOPs: No SOPs

Data files: HepG2 CoA labelling data, palmitate/no-glucose ..., HepG2 acyl-CoA measurements, palmitate/low-gluc..., HepG2 acyl-CoA measurements, palmitate/no-gluco..., HepG2 acyl-CoA/acylcarnitine measurements stati..., HepG2 acyl-CoA/acylcarnitine measurements stati..., HepG2 acylcarnitine measurements, palmitate/low..., HepG2 acylcarnitine measurements, palmitate/no-..., HepG2 qPCR statistical significance, palmitate/..., HepG2 qPCR statistical significance, palmitate/..., HepG2 qPCR, palmitate/low-glucose (Fig. S11), HepG2 qPCR, palmitate/no-glucose (Fig. S9)

Snapshots: No snapshots

Created: 9th May 2025 at 14:50, Last updated: 16th May 2025 at 11:45

MetNet

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: MetNet

Study: Confined cell migration along extracellular mat...

Organisms: No organisms

SOPs: No SOPs

Data files: All Metadata PF-IVM_v1.xlsx

Snapshots: No snapshots

Created: 15th May 2025 at 19:02, Last updated: 15th May 2025 at 19:03

Imaging Analysis - Data Linked

MetNet

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: MetNet

Study: Confined cell migration along extracellular mat...

Organisms: No organisms

SOPs: P.FRI-250515-V1_3DOpticalFlowAlgorithm.docx, P.FRI-250515-V1_IntravitalMircoscopyofTumorCell...

Data files: samples-publish_PF-IVM_A.IMG.xlsx, samples-publish_PF-IVM_D.IMG.xlsx

Snapshots: No snapshots

Created: 15th May 2025 at 18:36, Last updated: 15th May 2025 at 19:01

Intravital Microscopy

MetNet

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: MetNet

Study: Confined cell migration along extracellular mat...

Organisms: No organisms

SOPs: P.FRI-250515-V1_IntravitalMircoscopyofTumorCell...

Data files: samples-publish_PF-IVM_D.IMG.xlsx, samples-publish_PF-IVM_MUS.xlsx

Snapshots: No snapshots

Created: 15th May 2025 at 18:35, Last updated: 15th May 2025 at 18:58

Modeling - Data Linked

MetNet

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: MetNet

Study: Chromatin conformation, gene transcription, and...

Organisms: No organisms

SOPs: P.BAC-250424-V1_Modeling_ChromatinConformation.pdf

Data files: samples-publish_VB-CC_MDL.xlsx

Snapshots: No snapshots

Created: 24th Apr 2025 at 17:25, Last updated: 24th Apr 2025 at 18:07

MetNet

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: MetNet

Study: Chromatin conformation, gene transcription, and...

Organisms: No organisms

SOPs: No SOPs

Data files: All Metadata - VB_CC.xlsx

Snapshots: No snapshots

Created: 24th Apr 2025 at 18:04, Last updated: 24th Apr 2025 at 18:06

Chromatin Sequencing Analysis - Data Linked

MetNet

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: MetNet

Study: Chromatin conformation, gene transcription, and...

Organisms: No organisms

SOPs: P.BAC-250422-V1_Hi-C_ChromatinConformation.docx

Data files: samples-publish_VB-CC_A.CHRM.xlsx, samples-publish_VB-CC_D.SEQ.xlsx

Snapshots: No snapshots

Created: 24th Apr 2025 at 17:16, Last updated: 24th Apr 2025 at 18:04

Short Read Sequencing - Data Linked

MetNet

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: MetNet

Study: Chromatin conformation, gene transcription, and...

Organisms: No organisms

SOPs: P.BAC-250422-V1_Hi-C_ChromatinConformation.docx

Data files: samples-publish_VB-CC_D.SEQ.xlsx, samples-publish_VB-CC_DNA.xlsx

Snapshots: No snapshots

Created: 24th Apr 2025 at 17:15, Last updated: 24th Apr 2025 at 18:00

Library Creation - Metadata

MetNet

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: MetNet

Study: Chromatin conformation, gene transcription, and...

Organisms: No organisms

SOPs: P.BAC-250422-V1_Hi-C_ChromatinConformation.docx

Data files: samples-publish_VB-CC_CEL.xlsx, samples-publish_VB-CC_DNA.xlsx

Snapshots: No snapshots

Created: 24th Apr 2025 at 17:12, Last updated: 24th Apr 2025 at 17:55

Imaging Analysis - Data Linked

MetNet

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: MetNet

Study: Chromatin conformation, gene transcription, and...

Organisms: No organisms

SOPs: P.BAC-250422-V1_ChromatinElectronMicroscopy_Chr..., P.BAC-250422-V1_PWS_ChromatinConformation.docx, P.BAC-250422-V1_SMLM_ChromatinConformation.docx

Data files: samples-publish_VB-CC_A.IMG.xlsx, samples-publish_VB-CC_D.IMG.xlsx

Snapshots: No snapshots

Created: 24th Apr 2025 at 17:11, Last updated: 24th Apr 2025 at 17:52

Cell Imaging - Data Linked

MetNet

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: MetNet

Study: Chromatin conformation, gene transcription, and...

Organisms: No organisms

SOPs: P.BAC-250422-V1_ChromatinElectronMicroscopy_Chr..., P.BAC-250422-V1_PWS_ChromatinConformation.docx, P.BAC-250422-V1_SMLM_ChromatinConformation.docx

Data files: samples-publish_VB-CC_CEL.xlsx, samples-publish_VB-CC_D.IMG.xlsx

Snapshots: No snapshots

Created: 24th Apr 2025 at 17:10, Last updated: 24th Apr 2025 at 17:49

(v.1.17.0)

About FAIRDOMHub | Funding and Programmes | Credits | Terms & Conditions | Imprint | Cookie preferences

Copyright © 2008 - 2025 The University of Manchester and HITS gGmbH