TiMet WP1.1 qRT-PCR LD to LL and LD

Description

2 WT (Col, Ws) and 5 clock mutants, in biological duplicates, from three conditions: Diurnal cycle (12L/12D), Extended night (DD), Extended light (LL), harvest every 2 hours. Numbers are in transcript copy per cell, obtained ...

Flow cytometry is a laser-based, high-throughput single-cell analysis technique used to simultaneously measure multiple physical and fluorescent characteristics of cells or particles in suspension. This assay quantifies cell populations based on parameters such as cell size (forward scatter), granularity (side scatter), and fluorescence intensity of labeled surface or intracellular markers, enabling immunophenotyping, cell cycle analysis, viability assessment, and functional studies. Samples are ...

This assay captures administrative and clinical metadata associated with patient study visits, serving as a structured data collection framework rather than a biological measurement assay. It records visit-level contextual information such as visit dates, timepoints, subject identifiers, clinical site details, and protocol-relevant annotations that are essential for linking experimental samples and assay results to their proper clinical context. This metadata layer ensures traceability and data ...

A mouse challenge assay introduces a defined treatment, infection, or condition to experimental mice. It assesses physiological, immunological, or pathological responses to controlled exposures. Input: Experimental mouse model Output: Treated or exposed mouse sample for downstream analysis.

Cell sorting is a flow cytometry-based assay used to physically separate heterogeneous cell populations into distinct subpopulations based on specific cell surface or intracellular markers, enabling downstream functional, molecular, or phenotypic analyses. Cells are labeled with fluorescently conjugated antibodies or dyes, interrogated by laser excitation, and individually sorted into collection vessels using electrostatic deflection or microfluidic mechanisms according to predefined gating ...

Flow cytometry is a laser-based, high-throughput single-cell analysis technique used to simultaneously measure multiple physical and fluorescent characteristics of cells or particles in suspension. This assay quantifies cell populations based on parameters such as cell size (forward scatter), granularity (side scatter), and fluorescence intensity of labeled surface or intracellular markers, enabling immunophenotyping, cell cycle analysis, viability assessment, and functional studies. Samples are ...

This assay captures and standardizes the metadata associated with cell extraction procedures, documenting key experimental parameters such as cell type, passage number, extraction method, reagents used, and processing conditions applied during the preparation of cellular material for downstream analyses. It serves as a critical data governance step to ensure traceability, reproducibility, and quality control across experiments by recording provenance information for extracted cell samples. The ...

This assay captures metadata associated with tissue collection procedures, documenting critical provenance and contextual information for biological specimens obtained from study subjects. It records standardized parameters such as tissue type, anatomical source, collection method, preservation conditions, and relevant donor or sample identifiers to ensure traceability and reproducibility across downstream experimental workflows. This metadata serves as a foundational record linking collected ...

This assay captures administrative and clinical metadata associated with patient study visits, serving as a structured data collection framework rather than a biological measurement assay. It records visit-level contextual information such as visit dates, timepoints, subject identifiers, clinical site details, and protocol-relevant annotations that are essential for linking experimental samples and assay results to their proper clinical context. This metadata layer ensures traceability and data ...

This assay captures metadata associated with bacterial extraction procedures, documenting the experimental parameters, sample identifiers, reagents, and procedural conditions used during the isolation and extraction of bacterial material from biological or environmental samples. It serves as a standardized record-keeping component that accompanies downstream analytical assays, ensuring traceability and reproducibility by cataloging key variables such as sample source, extraction method, instrument ...

Flow cytometry is a laser-based, high-throughput single-cell analysis technique used to simultaneously measure multiple physical and fluorescent characteristics of cells or particles in suspension. This assay quantifies cell populations based on parameters such as cell size (forward scatter), granularity (side scatter), and fluorescence intensity of labeled surface or intracellular markers, enabling immunophenotyping, cell cycle analysis, viability assessment, and functional studies. Samples are ...

DNA extraction is a fundamental laboratory procedure used to isolate and purify genomic DNA from biological samples for downstream molecular analyses. This assay encompasses the collection of relevant metadata associated with the extraction process, including sample identifiers, source material type, extraction method or kit utilized, instrument parameters, operator information, and quality control metrics such as DNA yield and purity (e.g., A260/A280 ratios). Standardized metadata capture ensures ...

Short read sequencing is a high-throughput next-generation sequencing (NGS) approach used to determine the nucleotide sequence of DNA or RNA fragments, enabling detection of genetic variants, gene expression profiles, or genomic features depending on the library preparation strategy employed. This assay involves fragmentation of input nucleic acids, adapter ligation, and amplification prior to sequencing on short-read platforms (such as Illumina), generating millions of short sequence reads ...

This assay quantifies gene expression at single-cell resolution by linking individual cell transcriptomic profiles to associated experimental or clinical data, enabling integrative analysis across multiple data modalities. Single-cell RNA sequencing (scRNA-seq) technology is employed to capture and measure the transcriptional activity of individual cells, generating high-dimensional expression matrices that are subsequently processed through quality control, normalization, and dimensionality ...

Positron emission tomography combined with computed tomography (PET-CT) is a hybrid nuclear medicine imaging assay that simultaneously measures functional metabolic activity and anatomical structure within the body. Radioactive tracers (typically 18F-fluorodeoxyglucose, FDG) are administered to the subject, and their differential uptake is detected by the PET component to quantify metabolic or biochemical processes, while the CT component provides high-resolution structural localization. The "Data ...

Immunohistochemistry (IHC) is a histological technique that detects and localizes specific proteins or antigens within tissue sections through the use of labeled antibodies, enabling assessment of protein expression, distribution, and abundance in a spatially resolved context. Tissue samples are typically fixed, embedded, sectioned, and subjected to antigen retrieval prior to incubation with primary and secondary antibodies conjugated to detectable reporters such as enzymatic or fluorescent labels. ...

This assay captures administrative and clinical metadata associated with patient study visits, serving as a structured data collection framework rather than a biological measurement assay. It records visit-level contextual information such as visit dates, timepoints, subject identifiers, clinical site details, and protocol-relevant annotations that are essential for linking experimental samples and assay results to their proper clinical context. This metadata layer ensures traceability and data ...

This assay captures metadata associated with tissue collection procedures, documenting critical provenance and contextual information for biological specimens obtained from study subjects. It records standardized parameters such as tissue type, anatomical source, collection method, preservation conditions, and relevant donor or sample identifiers to ensure traceability and reproducibility across downstream experimental workflows. This metadata serves as a foundational record linking collected ...

This assay captures administrative and clinical metadata associated with patient study visits, serving as a structured data collection framework rather than a biological measurement assay. It records visit-level contextual information such as visit dates, timepoints, subject identifiers, clinical site details, and protocol-relevant annotations that are essential for linking experimental samples and assay results to their proper clinical context. This metadata layer ensures traceability and data ...

A titer assay designed to quantify the concentration or activity level of a specific biological agent (such as a virus, antibody, or other biomolecule) in a given sample, with results linked to associated experimental datasets for integrated analysis. The assay employs serial dilution methodology to determine the highest dilution at which a detectable response or activity is observed, providing a standardized endpoint titer value. Results are systematically recorded and cross-referenced with ...

This assay evaluates the binding affinity and interaction of test compounds or biological molecules (such as antibodies or Fc-fusion proteins) to Fc receptors (FcRs), which are critical mediators of immune effector functions including antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis. Binding interactions are quantified using established biochemical or biophysical detection methodologies, enabling characterization of Fc-FcR engagement relevant to the functional activity of ...

Antibody-dependent neutrophil phagocytosis evaluates antibody-mediated uptake of targets by neutrophils. It measures fluorescence or imaging-based signals to assess innate immune activity. Input: Antibody-labeled target cells and neutrophils Output: Phagocytosis data file indicating neutrophil uptake efficiency.

Antibody-dependent cellular phagocytosis measures immune cell uptake of antibody-coated targets. The assay quantifies phagocytic activity as an indicator of antibody function and cellular response. Input: Antibody-treated target cells and effector cells Output: Phagocytosis data file (e.g., fluorescence or imaging-based readouts).