SOPs

We have adapted the definitions of terms in [ISA best practice][1] and [programmes and projects][2]:

Programme = Overarching research theme (The Digital Salmon)
Project = Research grant (DigiSal, GenoSysFat)
Investigation = a particular biological process, phenomenon or thing
(typically corresponds to [plans for] one or more closely related papers)
Study = experiment whose design reflects a specific biological research question
Assay = standardized measurement or diagnostic experiment using a
...

Extraction and quantitative analysis of proteins from FFPE tissue

Methods for GC ToF sample preparation: supernatant, cell pellets, derivatization, data acquisition, peak identification

Enzymatic MTP-based D-xylose assay using XDH/XMR enzyme mix from Megazymes D-xylose kit

Initial configuration and parameters of the Bioleaching

This file describes the naming system used to uniquely identify samples

Method extraction of intracellular metabolites in Lactococcus lactis

Method extraction of intracellular metabolites in Lactococcus lactis

Method for transformation of plasmids into Lactococcus lactis

Method for synthesis of LAB-medium sued for the SYSMO-LAB project

Method for analysis of various organic acids in the medium

Protein extraction, iTRAQ labeling, peptides separation, mass spectrometry and data analysis

A Standard Operating Procedure (SOP) is a document consisting of step-by-step information on how to execute a task. An existing SOP may need to just be modified and updated.

Use this template when you upload RNA sequencing data.
Email : 31 May 2016
More columns need to be added PE/SE etc?

Source: Tom Harvey
[E- mail from Jon Olav 19 May 2016]

This protocol details the RNAseq sample preparation starting with mRNA and ending with quality control of finished libraries. This protocol was used specifically for virus mRNA samples related to ORFeome lab group.

Source: Hanne Hellerud Hansen [ Email sent on May 2. 2016]

This protocol was followed for library prep in https://fairdomhub.org/data_files/1341
The file explains the lab procedure/protocol that was followed in the lab for "TruSeq stranded mRNA sample preparation" by Hanne Hellerud Hansen with comments.

Protocol for applying a glucose perturbation in Streptococcus pyogenes.

Perturbation of starved cells with glucose. Concentrations of intra- and extracellular metabolites are followed in time.

This HPLC method uses a isocratic method and a RI detector to identify and quantify almost all excreted catabolic metabolites.

This protocol for applying glucose perturbations works for Lactococcus lactis and Enterococcus faecalis

A protocol for acidic quenching of lactic acid bacteria used for analyses of intracellular metabolites.