SOPs

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230 SOPs visible to you, out of a total of 449

Measurement of the transmembrane pH gradient and thus pHi, when the external pH is known.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

A procedure to analyse the genomic interaction of E.coli RNA polymerase (RNAP) upon methylglyoxal (MG) stress, a toxic keto-aldehyde by-product of metabolism.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

SOP used for detecting non influential parameters and interactions in non linear dynamical models. These parameters can be estabilished which allows the prioritization of parameters that can be subsequently estimated using robust global optimizations methods.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

The model describes the series of chemical reactions in MG detoxification pathway, allowing one to predict the flux of all compounds produced during detoxification.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

Mathematical model of pH buffering, which allows the prediction of how the buffering capacity depends on the cytoplasm's composition, for any number of buffers.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

Method of how to perform the purification of glyoxalase II as well as kinetics assays.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

A method of how to measure methylglyoxal present in the growth medium.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

Measurements of K+ retained in the cytoplasm using flame photometry.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

The fluorescent DNA-binding dye used in qRT-PCR binds to all kinds of double stranded DNA. To prevent false-positive results, the RNA is treated with DNase to remove remaining DNA.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

Quantitative real-time PRC is used to compare kdpFABC expression between the E. coli strains MG1655 (wildtype)and MG1655 (kdpA4, a kdpFABC-inactive mutant after a shift to K+ limitation.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

A method to compare kdpFABC expression between MG1655 (wildtype) and MG1655 (kdpA4) after a shift to K+ limitation, the RNA was extracted from samples taken at different points in time.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

Pulsed-FRAP measure the diffusion constants in confined volumes in small cells like E. coli and other bacteria or cellular organelles.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

A procedure to measure the levels of GSH and SLG before and after exposure to MG using formic acid. A similar expreimental set up as for potassium efflux experiments is used to which a silicon oil centrifugation step is incorporated. Samples are analysed by LC-MS-MS in order to quantify intracellular GSH and SLG levels.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

The reverse transcriptase synthesizes DNA, which complements the mRNA template.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

To induce kdpFABC expression, cells are cultivated in K10 minimal medium (10mM K+) were shifted into K+ limiting growth medium (K0), containing 20 μM K+ by filtration.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

FRAP experiments are used for studying cytoplasmic diffusion in cells and cells membrane.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

Growing Escherichia coli to express KefF by adding IPTG for purification and kinetics experiments.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

Generating gene knock-ins using MJF618 expressing the defective lambdoid prophage recombination system λ red.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

Generating gene knock-outs using DY330 expressing the defective lambdoid prophage recombination system λ red.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

Experimental system that is designed to observe the in vivo stability and aggregation of a protein of interest.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

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