Data files

RNA-Seq raw data + sample description

Aiming a greater lactate production and a subsequent steering towards MCC, this operation was conducted. This reactor configuration was similar to the CelluloseXylan_SBR_2ndStage. The first period comprised a feeding phase (6 min; 67% of total feeding) and a reaction phase (4h), which were designed to produce lactate (high load, short time). Then, in the second period a feeding phase (3 min; 33 % of total feeding) and reaction phase (48h) were designed to target the MCC.

In this operation, an SBR fermenting cellulose and xylan was conducted with the aim of enhancing MCC production in two stages: a first stage (CelluloseXylan_SBR_1stStage) targeting lactate and a 2nd stage (current file) targeting MCC. This reactor was inoculated with biomass from CelluloseXylan_SBR_1

In this operation, an SBR fermenting cellulose and xylan was conducted with the aim of enhancing MCC production in two stages: a first stage (current file) targeting lactate and a 2nd stage (CelluloseXylan_SBR_2ndStage) targeting MCC

In this operation, an SBR fermenting cellulose and xylan was conducted with the aim of enhancing MCC production by shortening the HRT

Main parameters of the characterisation of the inoculum from the Lugo WWTP and cow rumen

Concentration of micronutrients and macronutrients added in the reactor feedstock

Batch sample publishing

This is a script to retrieve the beta-values for a specified genome region (hg38) from one Infinium Methylation array measurement. Input files for one sample are required to be in IDAT-format (RED + GREEN). For further information see README.txt

Absorption at 340 nm measured over time in assays performed to determine the IC50 of fruc-1,6-BP for various PGMs as well as calculations perfomed to determine the specific activity [%] normalized to the activity in absence of fruc-1,6-BP

AUC of measured EIC of glc-1,6-BP for each reaction supernatant measured for fig 6b as well as the calculated normalized AUC (normalized to the control).

Data depicted in fig S1

Raw data of absorption at 340 nm during the enzyme assays performed for fig 5 as well as calculations to generate specific activities (inpresence and absence of 100 µM fruc-1,6-BP) from raw data.

Data depicted in fig S2

Raw data of absorption at 340 nm during the enzyme assays performed for fig 2 and 3 as well as calculations to generate specific activities from raw data.

Raw data of absorption at 340 nm during the enzyme assays performed for fig 4 as well as calculations to generate specific activities from raw data.

Raw data of absorption at 340 nm during the enzyme assays performed for fig 5 as well as calculations to generate specific activities and fold changes from raw data.

Raw data of absorption at 340 nm during the enzyme assays performed for fig 6b as well as calculations to generate specific activities and fold changes from raw data.

phylogenetic tree data depicted in fig 1a

phylogenetic tree data depicted in fig 1b

Specific activites of PGM enzymes with and without 40 µM glc-1,6-BP/ 100 µM fruc-1,6-BP.

Specific activites of PGM enzymes with and without 40 µM glc-1,6-BP/ 100 µM fruc-1,6-BP.

Proteinsequences used to generate the phylogenetic trees of fig 1

Batch sample publishing

Batch sample publishing

Batch sample publishing

Batch sample publishing

public file from researchdrive