SOPs
Protein extraction, iTRAQ labeling, peptides separation, mass spectrometry and data analysis
Creators: Theresa Kouril, Phil Wright, Trong Khoa Pham
Submitter: Theresa Kouril
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A Standard Operating Procedure (SOP) is a document consisting of step-by-step information on how to execute a task. An existing SOP may need to just be modified and updated.
Creator: Olga Krebs
Submitter: Deleted submitter
Protocol for applying a glucose perturbation in Streptococcus pyogenes.
Creator: Martijn Bekker
Submitter: Martijn Bekker
Perturbation of starved cells with glucose. Concentrations of intra- and extracellular metabolites are followed in time.
Creator: Martijn Bekker
Submitter: Martijn Bekker
This HPLC method uses a isocratic method and a RI detector to identify and quantify almost all excreted catabolic metabolites.
Creator: Martijn Bekker
Submitter: Martijn Bekker
A protocol for acidic quenching of lactic acid bacteria used for analyses of intracellular metabolites.
Creator: Martijn Bekker
Submitter: Martijn Bekker
This protocol for applying glucose perturbations works for Lactococcus lactis and Enterococcus faecalis
Creator: Martijn Bekker
Submitter: Martijn Bekker
Matlab script that reproduces the model redesign results outlined in RobOKoD: microbial strain design for (over)production of target compounds (http://fairdomhub.org/publications/236).
Creator: Natalie Stanford
Submitter: Natalie Stanford
Matlab script that reproduces the model redesign results outlined in RobOKoD: microbial strain design for (over)production of target compounds (http://fairdomhub.org/publications/236) for OptKnock.
Creator: Natalie Stanford
Submitter: Natalie Stanford
Matlab script that reproduces the model redesign results outlined in RobOKoD: microbial strain design for (over)production of target compounds (http://fairdomhub.org/publications/236) for RobustKnock.
Creator: Natalie Stanford
Submitter: Natalie Stanford
SOP for the determination of external metabolites (Glc, Pyr, Gly, Lac) in intact trophozoite incubations, and for the determination of intracellular metabolite concentrations.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for the cultivation conditions of Plasmodium falciparum, including the protocol for synchronisation.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of G3PDH activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of ALD activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of glucose transport activity in intact trophozoites.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of GAPDH activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of ENO activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of HK activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of PFK activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of LDH activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of PGK activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of PK activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of PGM activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
