Fc-engineered antibodies promote neutrophil-dependent control of Mycobacterium tuberculosis

About

This project houses all the publicly available datasets generated by the Hi-IMPAcTB consortium over the course of its 7+ years.

What is Hi-IMPAcTB?

Hi-IMPAcTB is an ambitious endeavor bringing together a team of international, interdisciplinary researchers to improve our understanding of host-pathogen interactions in the context of tuberculosis (TB). Funded by a multi-center contract from the NIH, the long-term goal of the consortium is to enable principled vaccine design by improving ...

This assay captures administrative and clinical metadata associated with patient study visits, serving as a structured data collection framework rather than a biological measurement assay. It records visit-level contextual information such as visit dates, timepoints, subject identifiers, clinical site details, and protocol-relevant annotations that are essential for linking experimental samples and assay results to their proper clinical context. This metadata layer ensures traceability and data ...

Library preparation converts extracted nucleic acids into sequencing-ready libraries. The process includes fragmentation, adapter ligation, and amplification to generate consistent input for sequencing instruments. Input: TIS, CEL, DNA or RNA sample Output: Indexed sequencing library suitable for sequencing assays.

A Luminex assay quantifies multiple analytes simultaneously using color-coded microspheres and fluorescence detection. The assay measures cytokines, antibodies, or proteins within a single multiplexed reaction. Input: Biological sample Output: Multiplex fluorescence data file representing analyte concentrations.

This assay characterizes the glycosylation profile of biological samples by analyzing the composition, structure, and abundance of glycan moieties attached to proteins or other biomolecules. Standard glycosylation analysis workflows typically involve sample preparation steps such as enzymatic release of N-linked glycans (e.g., via PNGase F treatment), followed by labeling or derivatization and separation using chromatographic or electrophoretic techniques (such as HPLC, UHPLC, or capillary ...

This assay employs an enzyme-linked immunosorbent assay (ELISA) methodology to quantitatively detect and measure specific analytes (such as proteins, antibodies, or other biomolecules) in biological samples. The technique utilizes antibody-antigen interactions coupled with enzymatic signal amplification to generate colorimetric or fluorometric readouts proportional to analyte concentration. Sample preparation, incubation conditions, wash steps, and detection reagents are performed according to ...

This assay evaluates the ability of bacterial populations to survive under defined restrictive conditions, quantifying survivability metrics through linked experimental data to assess bacterial viability and growth inhibition. The methodology incorporates controlled exposure of bacterial cultures to restrictive agents or environmental stressors, with outcomes measured via standardized enumeration or optical density-based approaches to determine survival rates relative to untreated controls. Results ...

Antibody-dependent neutrophil phagocytosis evaluates antibody-mediated uptake of targets by neutrophils. It measures fluorescence or imaging-based signals to assess innate immune activity. Input: Antibody-labeled target cells and neutrophils Output: Phagocytosis data file indicating neutrophil uptake efficiency.

Antibody-dependent NK cell activation measures natural killer (NK) cell responses triggered by antibody-coated targets. The assay detects cytokine release or surface marker expression as indicators of immune activation. Input: Antibody-treated target cells and NK effector cells Output: Cytometric or fluorescence data representing NK activation levels.

Antibody-dependent cellular phagocytosis measures immune cell uptake of antibody-coated targets. The assay quantifies phagocytic activity as an indicator of antibody function and cellular response. Input: Antibody-treated target cells and effector cells Output: Phagocytosis data file (e.g., fluorescence or imaging-based readouts).

Antibody-dependent complement deposition measures complement protein binding following antibody interaction. The assay detects complement activation as an indicator of antibody-mediated immune function. Input: Antibody-coated target cells Output: Complement deposition measurement or fluorescence data file.

This assay quantifies gene expression at single-cell resolution by linking individual cell transcriptomic profiles to associated experimental or clinical data, enabling integrative analysis across multiple data modalities. Single-cell RNA sequencing (scRNA-seq) technology is employed to capture and measure the transcriptional activity of individual cells, generating high-dimensional expression matrices that are subsequently processed through quality control, normalization, and dimensionality ...

Single cell clustering is a computational analysis assay that groups individual cells into distinct clusters based on transcriptomic or multi-omic feature similarity, enabling the identification and characterization of cell populations within heterogeneous samples. This data-linked implementation integrates clustering outputs with associated metadata and upstream single-cell sequencing data (such as scRNA-seq), applying algorithms such as k-means, graph-based (e.g., Louvain/Leiden), or hierarchical ...

A bacterial challenge assay exposes biological samples or model organisms to a defined bacterial strain. It is used to study infection dynamics, host-pathogen interactions, or immune response under controlled conditions. Input: Biological system or culture Output: Treated sample or response dataset reflecting bacterial exposure effects.

Batch sample publishing

Batch sample publishing

Batch sample publishing

Batch sample publishing

Batch sample publishing

Batch sample publishing

Batch sample publishing

Batch sample publishing

Batch sample publishing

Batch sample publishing

Batch sample publishing

Batch sample publishing

Batch sample publishing

Batch sample publishing

Batch sample publishing

Batch sample publishing

Batch sample publishing