SOPs

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230 SOPs visible to you, out of a total of 449
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This protocol is designed to prepare sufficient amounts of high-quality total RNA from the yeast saccharomyces cerevisiae grown in liquid culture for analysis on microarrays.

Creators: Walter Glaser, Christa Gregori

Submitter: Walter Glaser

Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 2. Membrane proteins

Creator: Miguel Curto Rubio

Submitter: The JERM Harvester

Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 1. Citosoluble proteins

Creator: Miguel Curto Rubio

Submitter: The JERM Harvester

Purpose: Cation content analysis - protocol

Creator: Clara Navarrete

Submitter: The JERM Harvester

Purpose:

Creator: Jose Ramos

Submitter: The JERM Harvester

SOPs related with medium composition, fermentation and stocking of S. solfataricus.

MIAPE (Minimum Information About a Proteomics Experiment) is the recommended format for proteomics data in SysMO-SEEK. The document attached provides more information and links to tools and resources.

Standard procedure regarding intracellular metabolome analysis using GC-MS.

Protocol for RNA isolation, cDNA synthesis and labeling and hybridization and cleanup of Sulfolobus solfataricus microarray

Standard operating procedures regarding iTraq based proteomics.

A model to describe the effect of K+ uptake by KdpFABC on the two-component system KdpD/KdpE, which enables the identification of control principles of the Kdp system

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

The reverse transcriptase synthesizes DNA, which complements the mRNA template (complementary DNA, cDNA). Cy3/Cy5-dCTP are incorporated into cDNA during Reverse transcription. The obtained Cy3/Cy5 cDNA are then competitively hybridised onto Agilent microarray slide and subsequently scanned.

Cells were harvested from culture keeping the cells cold to quench the physiological condition of RNA and the cells were mechanically disrupted. RNA was isolated from the cells by conventional acid-phenol method and the quality was checked by Agilent bioanalyser.

This SOP describes the SUMO procedure for determining B-galactosidase activities.

A kinetic model that describess the activation of a dimeric efflux system that could bind either GSH or SLG

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

This SOP defines the format of SOPs used in the SUMO consortium.

Creator: Michael Ederer

Submitter: Michael Ederer

A model for translation elongation, which allows the prediction of how different conditions and parameters affect the rate and throughput of translation.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

A model to describe aggregation of homomeric protein complexes in mechanosensitive channels in E.coli.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

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