Linear Fits and Michaelis Menten plot (pdf) Version 1
This is the pdf of the Jupyter Notebook to allow looking at the notebook without installing anything.
This Jupyter notebook needs to be located in the same place as a folder "data" and a folder "classes" to run.
"data" must contain the csv with the raw data, see the example data that is provided (MM25nMGre2pNDKNADPHabsorbance340nm_cleaned.csv).
"classes" must contain the file "MMKinetics.py", which is provided here on FAIRDOMhub.
The notebook load the functions from MMKinetics.py and reads in the csv file.
It requires some user input, as explained in the notebook, to run an analysis.
It creates 3 plots in total. Figurelegends to the plot can be edited in "MMKinetics.py".
SEEK ID: https://fairdomhub.org/sops/506?version=1
Filename:
MichaelisMentenNotebook.pdf
Format: PDF document
Size: 3.06 MB
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Views: 92 Downloads: 6
Created: 11th Oct 2021 at 12:02
Last updated: 12th Oct 2021 at 08:54
Last used: 4th Dec 2021 at 20:02
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Version 1 Created 11th Oct 2021 at 12:02 by Gudrun Gygli
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https://orcid.org/0000-0001-9119-1778Polyglot European Scientist. I thrive working in interdisciplinary environments combining the study of enzyme reactions and mechanisms with bioinformatics, molecular modelling, automated data analysis and data stewardship.
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Projects: Test Project (dummy), Towards Reproducible Enzyme Modeling
Web page: Not specified
Investigation 1: An experimental workflow to provide detailed information of the molecular mechanisms of enzymes is described. This workflow will help in the application of enzymes in technical processes by providing crucial parameters needed to plan, model and implement biocatalytic processes more efficiently. These parameters are homogeneity of the enzyme sample (HES), kinetic and thermodynamic parameters of enzyme kinetics and binding of reactants to enzymes. The techniques used to measure ...
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This Jupyter Notebook assists you in the analysis of initial rate experiments.
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For documentation about Juypter Notebooks, see here: https://jupyter-notebook.readthedocs.io/en/stable/
There are multiple tutorials online that help you to learn how to use a Jupyter notebook.
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Submitter: Gudrun Gygli
Biological problem addressed: Model Analysis Type
Investigation: On the reproducibility of enzyme reactions and ...
