Institutions: Consiglio Nazionale delle Ricerchehttps://orcid.org/0000-0002-3399-7973
Institutions: INSTITUTE FOR PLANT MOLECULAR AND CELL BIOLOGY (IBMCP)https://orcid.org/0000-0001-8589-7002
I'm currently a Postdoc at the Institute of Technical Biochemistry in Stuttgart University. My project involves the experimental validation of the Indirect Enzymatic Dehydration Via Phosphorylation and Dephosphorylation of Isobutanol for Isobutene production.
Projects: Not specified
Institutions: Not specifiedhttps://orcid.org/0000-0003-2852-6648
I am performing research in biomarkers discovery, particularly regarding liver fibrosis in non-alcoholic fatty liver disease. My research activities involved the use of bioinformatic platforms, the analysis of biological protein-protein interaction networks and the experimental validation of candidates by ELISA.
Assist. Prof. of Enzyme Technology Laboratory, Department of Chemistry, University of Crete, Greece
Projects: Sustainable co-production
Institutions: University of Rostockhttps://orcid.org/0000-0001-5393-3267
Projects: Not specified
Institutions: Not specifiedhttps://orcid.org/0000-0001-8357-2159
Expertise: Biochemical analysis, ELISA, oral food challenge, food allergy, Microbiology, protein extraction, Immunoblots, Data analysis, peanut and treenut allergy, protein purification, Immunoassays, Mass spectrometry (LC-MS/MS), Molecular Biology
Tools: Biochemistry and protein analysis, immunoblot, oral food challenge matrix preparation for food allergy research, protein purification, molecular biology techniques, ELISA Techniques, data analysis and data management, food allergen characterisation, 1D and 2D SDSPAGE, Immunological techniques, Mass spectrometry
Institutions: Wageningen University & Research
Projects: Not specified
Institutions: Not specifiedhttps://orcid.org/0000-0002-6586-717X
Research scientist in multi-Omics, molecular (cell)biology and bioinformatics.
Institutions: University of Milano-Bicocca
I am a PostDoc working on yeast metabolomics. During my PhD I studied the interplay between metabolism, cell cycle and signalling, mainly focusing on the Snf1/AMPK pathway. I am currently interested in studying metabolic rewiring caused by different nutrients, generating high-throughput data suitable for modelling.
Tools: molecular biological techniques (RNA/DNA techniques analyses of transport across cell membranes heterologous expression of transporters, mutant strain generation, Fluorecence based reporter gene analyses/single cell analyses, Biochemistry and protein analysis
biochemistry, molecular biology and physiology of lower eukaryotes,
characterization of cell membrane transporters
I am assistant professor at the Laboratory of Microbiology and my interest is in the area of molecular microbiology. Research focuses on the analysis of the metabolism of anaerobic fermentative bacteria and archaea, especially with respect to biofuel production (hydrogen, butanol). Within SysMo our tasks concern the effect of butanol stress, using metabolomics and transcriptomics.
Tools: SPINE, bacterial two-hybrid system, SubtiWiki, Chemical cross-linking, mutant strain generation, Proteomics (2D-PAGE), Genetic analysis, Genetic modification, Biochemistry and protein analysis, Molecular Biology, Microbiology
Tools: molecular biological techniques (RNA/DNA techniques), protein interaction studies, DNA technology RNA technology Protein analysis Fermentation Mutagenesis, gene regulation, Proteomics (2D-PAGE), Model organisms, Proteomics, Transcriptomics, Genetic analysis, Genomics, Cell biology, Biochemistry and protein analysis, Molecular Biology, Microbiology
Optimisation of Bacillus subtilis for the secretion of heterologous proteins Therapeutic proteins (including those required for experimental purposes and clinical trials) are major products of biomanufacturing processes and considerable time and expense are expended to maximise the yield and quality of proteins produced in heterologous hosts. The production host of choice is the Gram-negative bacterium Escherichia coli for which many strains and expression systems have been developed. However,
Expertise: quantative biology, Cell physiology, regulation of gene expression, Microbiology/ Protein chemistry/ Molecular Biology, Protein-DNA-interaction, Bacterial Cell Biology, carbon metabolism, bacterial metabolism, functional protein expression, Bacillus subtilis
Tools: surface plasmon resonance spectroscopy, reporter gene analysis, DNA, Molecular biology techniques (RNA/DNA/Protein), Chromatography, Model organisms, Cell biology, Biochemistry and protein analysis, Microbiology
Professor of Biochemistry at the Centre of Biochemistry of Heidelberg University, teaching biochemistry for medical and biology students
Research focus is the trypanothione redox metabolism of African trypanosomes (Trypanosoma brucei).
The work is funded within the Collaborative Research Centre 544 on "Control of Tropical Infectious Diseases" of the German Research Foundation
I am a research technician at the Institute of Medical Science in Aberdeen, working for Prof. Ian Booth. The topic of our workpackage deals with K+ homostasis in Escherichia coli. I am working with the protein KefF, a regulatory subunit of the potassium channel KefC.
Tools: Fluorescence and confocal microscopy FRAP/FLIP, Molecular biology techniques (RNA/DNA), quantitative western blot analysis, fluorescence spectroscopy, rtPCR, Spectroscopy and structural analysis, Biochemistry and protein analysis
The focus of our research is the protein biogenesis and how stress-related factors modulate it. Protein biogenesis in general comprises various processes, i.e., translation, protein folding, each of which responds differently to external stress stimuli. Using systems biology approaches we seek to understand the interplay between these processes in fine-tuning the protein pattern and proteins’ abundance under osmotic stress conditions.
The major theme of the research in my laboratory is bacterial gene regulation. We are interested in signal perception mechanisms (in particular oxygen); signal transduction (ligand induced protein confromational changes); interaction of transcription factors with the core transcription machinery; interactions between transcription factors to integrate multiple signals; and the influence of promoter architectures on these events. We are also interested in aome aspects of post-transcriptional
Tools: reporter gene analyses, spectroscopy, site-directed and random mutagenesis, plasmon resonance spectroscopy, molecular biological techniques (RNA/DNA techniques), Membrane protein biochemistry, Chromatography, Fermentation, Biochemistry and protein analysis, Genetics
I am a PhD student of the microbiology department at the Ludwig-Maximilians Universität München. I work at the chair of Prof. Kirsten Jung. The topic of our workpackage deals with "K+ homeostasis in Escherichia coli". In special I'm working on the sensor kinase KdpD that controls together with the response regulator KdpE the expression of the high-affinity K+ uptake system KdpFABC. The yet not fully understood molecular mechanism of stimulus perception and signal transduction is of particular
I am the foundation Professor of Systems Biology and Engineering within the Department of Chemical and Process Engineering (CPE), at The University of Sheffield. My research philosophy is centred on a mechanistic systems biology approach to solve biochemical reaction engineered processes. I wish to pursue issues involved in the effective utilisation of biological resources. The approach is specifically targeted at the conjunction of chemical engineering (metabolic engineering and synthetic biology),
Expertise: yeast, fungi, Metabolomics, Proteomics, Stoichiometric modelling, carbon metabolism, Systems Biology, dynamics and control of biological networks, coupling metabolome and environome, rapid sampling experiments, Biochemistry
Tools: parameter estimation, ODE, HPLC, GC and LC/MS analysis of metabolites, Mass spectrometry (LC-MS/MS), continuous cultivation, Enzyme assay, stimulus response experiments, Material balance based modeling, Chromatography, Fermentation, Matlab, Metabolomics, Biochemistry and protein analysis
I've become a SysMO DB PAL for MOSES project in 2007 being a post-doc in lab of Prof. Matthias Reuss at University of Stuttgart. In the MOSES project, our major efforts were in the experimental data acquisition for dynamic model of primary carbon and anaerobic energy metabolism in yeast. The model implements prediction of perturbations of two types: glucose pulse and temperature jump. We implement “stimulus-response” methodology for the unraveling the dynamic structure of the network and to
I am a PhD student working the group of Zoya Ignatova. Cellular and extracellular changes like crowding and osmotic stress conditions play a major role in protein aggregation. A change in the cytoplasmic composition is the result of an interplay between high osmotic pressures outside the cell volume and the cellular response to it in terms of uptake of K+ and secondary organic osmolytes. My research focuses on elucidating the role of natural osmolytes (known also as chemical chaperones or compatible
Professor in Jinan University, Guangzhou, China.
My research interest is in the modeling of translation. Connecting various processes in translation, we can investigate the impact of different factors on protein biosynthesis and biogenesis in genome-wide scale. This may reveal various general mechanisms on control level of gene expression and folding efficiency regulation in different growth conditions.
I'm Post-Doc in the lab of Prof. Becher at the University of Greifswald. I'm working on the relative and absolute protein quantitation using gel-based and mass-spectrometric methods.
Work in my laboratory is focussed on microbial physiology - the study of how bacteria and other microorganisms work. Although rooted in the tradition of bacterial growth and intermediary metabolism, microbial physiology now embraces molecular biology, genetics, biochemistry, and indeed any discipline that can shed light on bacterial function. Much of our experimental work is conducted with Escherichia coli, the pre-eminent ‘model’ organism with unrivalled ease of genetic and physiological
I obtained my PhD in 1989 at the Free University (Amsterdam) on a research project in which microbial physiology, biochemistry, and molecular biology were combined. Subsequently I spent 3 years abroad, 2.5 years of which as EMBO fellow at the EMBL (Heidelberg, Germany) where I worked on protein engineering and protein crystallization. I returned to Amsterdam as KNAW fellow for 3 years, during which I worked on protein analysis and pathway engineering. In 1995 I was appointed as group leader