CEPOPA envisions a sustainable cascade one-pot process for the synthesis of optically pure amines from alcohols and/or alkynes, using enzymes immobilized onto catalytically active nanomaterials. In the framework of the project, efficient chemoenzymatic processes will be developed for the synthesis of a set of amines that can be used as building blocks for pharmaceuticals or biomaterials.
Programme: Hellenic Foundation for Research and Innovation - Supporting academic Staff and Researchers
SEEK ID: https://fairdomhub.org/projects/193
Funding codes:- Project Number: 664
Public web page: Not specified
Organisms: Escherichia coli
FAIRDOM PALs: No PALs for this Project
Project start date: 24th Feb 2020
Project end date: 23rd Aug 2023
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- People (9)
- Programmes (1)
- Institutions (1)
- Investigations (5)
- Studies (0+5)
- Assays (0+4)
- Data files (5+6)
- SOPs (0+3)
- Publications (3)
- Presentations (1)
- Events (1)
- Documents (1+3)
Assist. Prof. of Enzyme Technology Laboratory, Department of Chemistry, University of Crete, Greece
The Hellenic Foundation for Research and Innovation is a new institution through which a profound reforming effort is being undertaken in the field of Research and Innovation in the country. The Organization supports unrestricted research (the systematic inquiry born of scientific curiosity) and new researchers by providing scholarships for doctoral candidates, as well as research projects for post-doctoral students, Researchers and Faculty Members. Through H.F.R.I., the research and academic ...
Web page: http://www.elidek.gr/en/homepage/
Objectives: Empowering smooth implementation and fruitful completion of all WPs and tasks. Implementation of a data management plan for efficient dissemination under F.A.I.R. principles.
Description of Work: The PI of the project with the heads of the collaborating groups will closely monitor the progress of the technical and administrative tasks and it will implement actions to correct any deviation from the established work-plan. The whole group will meet regularly every six months or more ...
Snapshots: No snapshots
Objectives: Establishment of the chemoenzymatic process with the best GO-ATA hybrid catalysts. Highlighting of the potential of the process in semi-preparative scale.
Description of Work: The best hybrid catalysts identified in WP3 will be investigated in coupled one-pot reactions selected in WP1, in batch and continuous flow reactors. The productivity of the system will be optimized with response surface methodology (RSM), for parameters such as temperature, duration, substrate concentration ...
Snapshots: No snapshots
Objectives: Development of efficient ATA immobilization approach. Production of a hybrid catalyst of high catalytic efficiency.
Description of Work: Τhe ATAs selected in WP2 will be expressed, purified and covalently and/or non-covalently immobilized on the GO selected in WP1. The catalytic behavior (in terms of catalytic activity, stability and reusability) and the structural implications of the immobilization will be investigated. For comparison purposes, the non-optimized ATAs (prior to the ...
Snapshots: No snapshots
Objectives: Identification of amine transaminases (ATAs) able to catalyze efficiently the amination of a desired set of ketones and aldehydes (expected products of GO oxidation). Optimization of the most potent biocatalysts, in terms of catalytic efficiency, stability, availability of functional groups for covalent immobilization.
Description of Work: ATAs from our construct selection (>30 wild-type and variants of both (R)- and (S)-enantioselectivity) with different substrate selectivity will ...
Snapshots: No snapshots
Objectives: Protocol identification and establishment for the synthesis of high-performing catalytic GO in the desired oxidation reactions under mild conditions. Characterization of the most prominent materials synthesized and comparison to commercially available material.
Description of Work: Several established chemical methods will be used for the synthesis of GO. Each batch will be characterized, for instance for its C/O ratio, surface area and conductivity. The catalytic profile of the ...
Snapshots: No snapshots
The structure of was Transaminase of gamma-proteobacterium deposited in PDB under the code: 7p3t
Creator: Eleni Konia
Submitter: Ioannis Metaxas
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Data of the activity/pH profile of the under study enzymes
Creator: Eleni Konia
Submitter: Ioannis Metaxas
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Data for the photometric determination of the activity for the under study enzymes.
Creator: Eleni Konia
Submitter: Ioannis Metaxas
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Data file for the Pyridoxamine phosphate formation assay
Creator: Eleni Konia
Submitter: Ioannis Metaxas
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Data files for the kinetic resolution assay
Creator: Eleni Konia
Submitter: Ioannis Metaxas
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Abstract (Expand)
Authors: Nikolaos Kaloudis, Panagiota Zygouri, Nikolaos Chalmpes, Konstantinos Spyrou, Dimitrios Gournis, Ioannis Pavlidis
Date Published: 6th Dec 2021
Publication Type: Journal
DOI: 10.3389/fctls.2021.803850
Citation:
Abstract (Expand)
Authors: Ioannis Metaxas, Emily Michailidi, Dimitris Stavrou, Ioannis V. Pavlidis
Date Published: 13th Jul 2021
Publication Type: Journal
Citation: Chemistry Teacher International 0(0),000010151520210011
Abstract (Expand)
Authors: Eleni Konia, Konstantinos Chatzicharalampous, Athina Drakonaki, Cornelia Muenke, Ulrich Ermler, Georgios Tsiotis, Ioannis V. Pavlidis
Date Published: 2021
Publication Type: Journal
DOI: 10.1039/D1CC04664K
Citation: Chem. Commun.,10.1039.D1CC04664K
Poster presented in Protein Engineering Congress EU
Creators: Ioannis Pavlidis, Eleni Konia, Nikolaos Kaloudis
Submitter: Ioannis Pavlidis
Protein Engineering Congress EU
Start Date: 24th Nov 2020
End Date: 1st Dec 2020
Event Website: https://www.proteinengineeringcongresseu.com/events/protein-engineering-congress-eu-2020
Country: Not specified
City: Not specified
Data Management Plan (v.1.0) of the project CEPOPA.
Investigations: No Investigations
Studies: No Studies
Assays: No Assays