Deliverable D2.2 Biochemical characterization of the most prominent ATAs

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The Hellenic Foundation for Research and Innovation is a new institution through which a profound reforming effort is being undertaken in the field of Research and Innovation in the country. The Organization supports unrestricted research (the systematic inquiry born of scientific curiosity) and new researchers by providing scholarships for doctoral candidates, as well as research projects for post-doctoral students, Researchers and Faculty Members. Through H.F.R.I., the research and academic ...

CEPOPA envisions a sustainable cascade one-pot process for the synthesis of optically pure amines from alcohols and/or alkynes, using enzymes immobilized onto catalytically active nanomaterials. In the framework of the project, efficient chemoenzymatic processes will be developed for the synthesis of a set of amines that can be used as building blocks for pharmaceuticals or biomaterials.

Objectives: Identification of amine transaminases (ATAs) able to catalyze efficiently the amination of a desired set of ketones and aldehydes (expected products of GO oxidation). Optimization of the most potent biocatalysts, in terms of catalytic efficiency, stability, availability of functional groups for covalent immobilization.

Description of Work: ATAs from our construct selection (>30 wild-type and variants of both (R)- and (S)-enantioselectivity) with different substrate selectivity will ...

Objectives: Protocol identification and establishment for the synthesis of high-performing catalytic GO in the desired oxidation reactions under mild conditions. Characterization of the most prominent materials synthesized and comparison to commercially available material.

Description of Work: Several established chemical methods will be used for the synthesis of GO. Each batch will be characterized, for instance for its C/O ratio, surface area and conductivity. The catalytic profile of the ...