Silvio Hering
SEEK ID: https://fairdomhub.org/people/353
Location:
Germany
ORCID: Not specified
Joined: 1st Oct 2010
Expertise: Molecular microbiology
Tools: Biochemistry and protein analysis, Genetic modification, Metabolomics
Their tagsRelated items
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep
Web page: http://sysmo.net/
Comparative Systems Biology: Lactic Acid Bacteria
Programme: SysMO
Public web page: http://www.sysmo.net/index.php?index=57
The Lactate dehydrogenases (LDH) are key metabolic enzymes in lactic acid bacteria (LAB). The LDH ( E.C. 1.1.1.27) catalyzes the reaction of pyruvate and NADH into lactate and NAD+.We have carried out an experimental and computational study of the effects of fructose-1,6-bisphosphate (FBP), phosphate (Pi) and ionic strength (NaCl concentration) on 3 LDHs from 3 LABs studied at pH 6 and pH 7.
Submitter: Silvio Hering
Investigation: Investigation of glycolysis and pyruvate branch...
Assays: Kinetics of L-lactate dehydrogenase from S. pyogenes, E. faecalis, and L...
S. pyogenes was grown in C-limited cultures at pH 6.5 and 7.5 and at a growth rate of 0.05 The glnA mutant strain shows decreased growth in low glutamine and excess glutamate conditions and no growth at all in low glutamine and low glutamate conditions. Preliminary results of glucose-limited chemostat cultures indicate a reversion of the pH dependency of the shift from homolactic to more mixed acid fermentation: wild type - lactate/formate ratio at pH 6.5 = 11.8, at pH 7.5 = 2.8 glnA mutant - ...
Creators: Antje Sieg, Silvio Hering, Martijn Bekker
Submitter: Antje Sieg
DownloadHeterologous Expression of LDHs from different lactic acid bacteria in Escherichia coli DH5α. Assessment of kinetic parameters of LDH to include in a catabolic model .
Creators: None
Submitter: Silvio Hering
Heterologous Expression of LDHs from different lactic acid bacteria in Escherichia coli DH5α.
Assessment of kinetic parameters of LDH to include in a catabolic model.
Creators: Silvio Hering, Tomas Fiedler
Submitter: Silvio Hering
