Postdoc @ Institute of Medical Microbiology, University of Rostock
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- Assays (1)
- Data files (6)
- SOPs (3)
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S. pyogenes M49 (591), E. faecalis V583, and L. lacis NZ9000 and their isogenic ldh deletion mutants were grown glucose free CDM-LAB medium in BIOLOG phenotype microarray plates PM01 and PM02. With this assay the abilitiy of the strains to grow on 190 different carbon sources was determined in 96 well format.
Contributor: Tomas Fiedler
Assay type: Experimental Assay Type
Technology type: Technology Type
Snapshots: No snapshots
Investigation: Investigation of glycolysis and pyruvate branch...
Organisms: Lactic Acid Bacteria (batch)
SOPs: No SOPs
Data files: No Data files
. L. lactis (NZ9000), E. faecalis (V538) and S. pyogenes (M49) wild type strain and their ldh- mutants were grown in batch cultures at 37°C in anaerobic 96 wells plates in either TH-broth supplemented with 0.5% (w/v) yeast (THY) or a chemically defined medium for LAB (pH 7.4) (CDM-LAB (10)). Both media were buffered with either 100 mM MES buffer or 100 mM MOPS buffer for growth at pH 6.5 and 7.5 respectively.
S. pyogenes was grown in C-limited cultures at pH 6.7 and 7.5 and at a growth rate of 0.05 and 0.15
Heterologous Expression of LDHs from different lactic acid bacteria in Escherichia coli DH5α.
Assessment of kinetic parameters of LDH to include in a catabolic model.
Investigations: The effect of pH upon the metabolic shift in Cl...
Studies: Effect of pH on the proteome
Date Published: 1st Jul 2013
Journal: Protein Expression and Purification
Authors: Renate Kania, Maja Rey, Olga Krebs, Isabel Rojas, Wolfgang Müller, Wittig Ulrike,Golebiewski Martin,Shi Lei,Jong Lenneke,Algaa Enkhjargal,Weidemann Andreas,Sauer-Danzwith Heidrun,Mir Saqib,Bittkowski Meik,Wetsch Elina
Date Published: 18th Nov 2011
Journal: Nucleic Acids Res.
PubMed ID: 22102587
Date Published: 14th Feb 2012
Journal: The FEBS journal
PubMed ID: 22325620
Date Published: 1st Nov 2011
Journal: Applied and environmental microbiology
PubMed ID: 22038603
Date Published: 8th Feb 2011
Journal: Not specified
PubMed ID: 21296946
Date Published: 8th Dec 2010
Journal: Future Microbiol
PubMed ID: 21133689