Professor in biotechnology at the Dept. Chemistry, Biotechnology and Food Science. I am heading "Laboratory of microbial gene technology and food microbiology" that consists of approximately 20 members (staff members, technicians,and students). During the last 20 years my research has been focused on lactica acid bacteria with a focus on bacteriocins of lactic acid bacteria.These studies have included purification and chemical and genetic characterization of such peptides followed by biosynthesis and regulation of biosynthesis through a quorum sensing mechanism. Structure, mode of action and receptor studies have been an important part of my research and recently we showed that the classIIa and other one-peptid bacteriocins actually are using the ManCD that is part of the man-PTS system, as receptors.
Modern genomic tools (genomic DNNA microarrays) are now used in both CGH and transcriptome analysis of Enterococcus faecalis and Lactobacillus sake. In our studying of stress responses and mutants, the array technology has been very valuable for looking into the transcriptional responses of such manipulations and to understand the mechanisms of adaptation. Proteomic techiques (2-D gel and MS analysis) are now also used in such studies.
SEEK ID: https://fairdomhub.org/people/47
Tools: gel-based and gel-free proteomics, 2-D Gel Electrophoresis, Mutant and Strain Construction, bacterial chemostat culture, qRT-PCR, molecular biological techniques (RNA/DNA techniques, Chromatography
ORCID: Not specified
A reconstruction of the cellular metabolism of the opportunistic human pathogen Enterococcus faecalis V583 represented as stoichiometric model and analysed using constraint-based modelling approaches
Contributor: Nadine Veith
Model type: Linear equations
Model format: SBML
Environment: Not specified
Organism: Enterococcus faecalis
Investigations: Investigation of glycolysis and pyruvate branch...
Modelling analyses: Genome-Scale Model Enterococcus faecalis V583
Date Published: 19th Dec 2014
Journal: Appl Environ Microbiol
PubMed ID: 25527553
Citation: Appl Environ Microbiol. 2015 Mar 1;81(5):1622-33. doi: 10.1128/AEM.03279-14. Epub 2014 Dec 19.
Date Published: 1st Nov 2011
Journal: Applied and environmental microbiology
PubMed ID: 22038603
Date Published: 8th Feb 2011
Journal: Not specified
PubMed ID: 21296946
Date Published: 25th Nov 2010
Journal: Not specified
PubMed ID: 21097579
Date Published: 22nd May 2009
Journal: Appl. Environ. Microbiol.
PubMed ID: 19465534