SysMO-LAB (Manchester Centre for Integrative Systems Biology, University of Manchester) ; MOSES (Manchester Centre for Integrative Systems Biology, University of Manchester) ; PSYSMO (Manchester Centre for Integrative Systems Biology, University of Manchester) ; MOSES (VU University Amsterdam) ; SulfoSys (VU University Amsterdam) ; SulfoSys - Biotec (VU University Amsterdam) ; EraCoBiotech 2 nd call proposal preparation (University of Amsterdam)
Within the e:Bio - Innovationswettbewerb Systembiologie (Federal Ministry of Education and Research (BMBF)), the SulfoSYSBIOTECH consortium (10 partners), aim to unravel the complexity and regulation of the carbon metabolic network of the thermoacidophilic archaeon Sulfolobus solfataricus (optimal growth at 80°C and pH 3) in order to provide new catalysts ‘extremozymes’ for utilization in White Biotechnology.
Based on the available S. solfataricus genome scale metabolic model (Ulas et al., 2012)
Systems analysis of process-induced stresses: towards a quantum increase in process performance of Pseudomonas putida as the cell factory of choice for white biotechnology.
The specific goal of this project is to exploit the full biotechnological efficacy of Pseudomonas putida KT2440 by developing new optimization strategies that increase its performance through a systems biology understanding of key metabolic and regulatory parameters that control callular responses to key stresses generated
MOSES (Micro Organism Systems biology: Energy and Saccharomyces cerevisiae) develops a new Systems Biology approach, which is called 'domino systems biology'. It uses this to unravel the role of cellular free energy ('ATP') in the control and regulation of cell function. MOSES operates though continuous iterations between partner groups through a new systems-biology driven data-management workflow. MOSES also tries to serve as a substrate for three or more other SYSMO programs.
The principles of Stealthy Engineering (Adamczyk et al.: Biotechnology Journal 2012; 7(7):877-83) are illustrated in this model by emulating a cross engineering intervention between L. lactis and S. cerevisiae.
The case study consists of replacing the native glucose uptake system of L. lactis with that native to the yeast S. cerevisiae. A modified version of Hoefnagel et al.’s model of L. lacrtis’ central metabolism was used as starting point. The total functional replacement of the PTS with the
Contributor: Ettore Murabito
Model type: Ordinary differential equations (ODE)
Model format: Copasi
Organism: Lactococcus lactis
Investigations: No Investigations
Studies: No Studies
Modelling analyses: No Modelling analyses
Date Published: 20th Jul 2013
Journal: FEBS J.
PubMed ID: 23865479
Date Published: 1st Sep 2012
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics
Date Published: 24th Aug 2011
Journal: Eur J Pharm Sci
PubMed ID: 21888969
Authors: Alexey Kolodkin, Fred C Boogerd, Nick Plant, Frank J Bruggeman, Valeri Goncharuk, Jeantine Lunshof, Rafael Moreno-Sanchez, Nilgun Yilmaz, Barbara M Bakker, Jacky Snoep, Rudi Balling, Hans Westerhoff
Date Published: 16th Jun 2011
Journal: Eur J Pharm Sci
PubMed ID: 21704158
Date Published: 6th May 2010
PubMed ID: 20444304
Date Published: 6th Nov 2009
Journal: FEBS Lett.
PubMed ID: 19913018