Heat stress response of the red-tide dinoflagellate Prorocentrum cordatum

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Pure cultures of P. cordatum were grown at 20°C, 26°C and 30°C until exponential and stationary growth phase and were harvested for metabolite extraction.

Online metabolite derivatization was performed using a Gerstel MPS2 autosampler (Muehlheim, Germany). Dried metabolites were dissolved in 15 µl of 2 % methoxyamine hydrochloride in pyridine at 40 °C under shaking. After 90 min, an equal volume of N-Methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA) was added and held for 30 min at 40 °C. One ...

Tryptic peptides of P. cordatum were separated by 2D SCX-/RP nanoLC coupled to ESI-Iontrap MS/MS.

The membrane fraction of Prorcentrum cordatum was prepared and proteins solubilized using SDS. Subsequently, proteins were separated by SDS-PAGE, subjected to tryptic in-gel digest and nanoLC ESI-iontrap MS/MS.

Prorocentrum cordatum was subjected to whole cell shotgun analyses (in-solution digest) unsing nanoLC ESI-intrap MS/MS

Detailed description of the methods applied for proteomic analyses of P. cordatum heat stress response

Detailed description of the methods applied for metabolomic analyses of P. cordatum heat stress response