Heat stress response of the red-tide dinoflagellate Prorocentrum cordatum
OverviewCells of P. cordatum adapted to growth at 20 °C and constant light:dark conditions were shifted to growth at 26 °C and 30 °C, respectively, with similar illumination conditions. During exponential and stationary growth, cells were collected for transcriptomic, proteomic and metabolomic analyses.
Programme: Independent Projects
SEEK ID: https://fairdomhub.org/projects/293
Public web page: Not specified
Organisms: Prorocentrum cordatum
FAIRDOM PALs: No PALs for this Project
Project created: 29th Jun 2022
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Projects: EbN1 Systems Biology, Heat stress response of the red-tide dinoflagellate Prorocentrum cordatum, The nucleus of Prorocentrum cordatum, Conspicuous chloroplast with LHC‒PSI/II‒megacomplex and diverse PBPs in the marine dinoflagellate Prorocentrum cordatum, Proteogneomics of Desulfobacteraceae members
Institutions: Carl von Ossietzky University of Oldenburg Institute for Chemistry and Biology of the Marine Environment (ICBM)
https://orcid.org/0000-0001-7253-3373
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Web page: Not specified
Submitter: Lars Wöhlbrand
Studies: Heat stress response of Prorocentrum cordatum
Assays: Metabolite analyses, Proteomic analyses
Submitter: Lars Wöhlbrand
Investigation: Heat stress response of Prorcentrum cordatum
Assays: Metabolite analyses, Proteomic analyses
Submitter: Lars Wöhlbrand
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Heat stress response of Prorcentrum cordatum
Submitter: Lars Wöhlbrand
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Heat stress response of Prorcentrum cordatum
Pure cultures of P. cordatum were grown at 20°C, 26°C and 30°C until exponential and stationary growth phase and were harvested for metabolite extraction.
Online metabolite derivatization was performed using a Gerstel MPS2 autosampler (Muehlheim, Germany). Dried metabolites were dissolved in 15 µl of 2 % methoxyamine hydrochloride in pyridine at 40 °C under shaking. After 90 min, an equal volume of N-Methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA) was added and held for 30 min at 40 °C. One ...
Creators: None
Submitter: Carsten Reuse
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Tryptic peptides of P. cordatum were separated by 2D SCX-/RP nanoLC coupled to ESI-Iontrap MS/MS.
Creators: None
Submitter: Lars Wöhlbrand
The membrane fraction of Prorcentrum cordatum was prepared and proteins solubilized using SDS. Subsequently, proteins were separated by SDS-PAGE, subjected to tryptic in-gel digest and nanoLC ESI-iontrap MS/MS.
Creators: None
Submitter: Lars Wöhlbrand
Prorocentrum cordatum was subjected to whole cell shotgun analyses (in-solution digest) unsing nanoLC ESI-intrap MS/MS
Creators: None
Submitter: Lars Wöhlbrand
Detailed description of the methods applied for proteomic analyses of P. cordatum heat stress response
Creator: Lars Wöhlbrand
Submitter: Lars Wöhlbrand
Detailed description of the methods applied for metabolomic analyses of P. cordatum heat stress response
Creator: Carsten Reuse
Submitter: Carsten Reuse
