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- Theresa Kouril
SEEK ID: https://fairdomhub.org/people/261
Location:
Germany
Expertise: Biochemistry, carbon metabolism, Carbohydrate Metabolism of Archaea, functional protein expression, Glycolysis, Microbiology, Molecular Biology, Systems Biology
Tools: Biochemistry, Molecular Biology, Enzyme assays
ORCID: Not specified
Related items
- Projects (2)
- Institutions (1)
- Investigations (3+1)
- Studies (6+1)
- Assays (10+1)
- Data files (23+3)
- SOPs (6)
- Publications (6)
- Samples (0+1)
Within the e:Bio - Innovationswettbewerb Systembiologie (Federal Ministry of Education and Research (BMBF)), the SulfoSYSBIOTECH consortium (10 partners), aim to unravel the complexity and regulation of the carbon metabolic network of the thermoacidophilic archaeon Sulfolobus solfataricus (optimal growth at 80°C and pH 3) in order to provide new catalysts ‘extremozymes’ for utilization in White Biotechnology.
Based on the available S. solfataricus genome scale metabolic model (Ulas et al., 2012)
...
Programme: e:Bio
Public web page: http://www.sulfosys.com/
Organisms: Sulfolobus solfataricus
Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation
Programme: SysMO
Public web page: http://sulfosys.com/
Organisms: Sulfolobus solfataricus
Integrated systems biology approach including transcriptome, metabolome, proteome analyses and modelling to elucidate amino acid degradation in S. solfataricus P2.
Snapshots: No snapshots
The gluconeogenic conversion of 3-phosphoglycerate via 1,3-bisphosphoglycerate to glyceraldehyde-3-phosphate was compared at 30 C and at 70 C. At 30 C it was possible to produce 1,3-bisphosphoglycerate from 3-phosphoglycerate with phosphoglycerate kinase, but at 70 C, 1,3- bisphosphoglycerate was dephosphorylated rapidly to 3-phosphoglycerate, effectively turning the phosphoglycerate kinase into a futile cycle. At both temperatures it was possible to convert 3-phosphoglycerate to glyceraldehyde
...
Snapshots: No snapshots
Integrated systems biology approach including transcriptome, metabolome, biochemistry, proteome analyses and modelling to elucidate the catabolic pathway for L-fucose in S. solfataricus P2.
Snapshots: No snapshots
To investigate amino acid degradation pathways in Sulfolobus solfataricus transcriptome, proteome and metabolome analyses were performed on cells grown on caseinhydrolysate as carbon source. Cells grown with glucose served as reference condition. Metabolic modelling was used to compare the efficiency of different degradation routes.
Person responsible: Jacqueline Wolf
Snapshots: No snapshots
PGK-GAPDH reactions were studied in vitro at 30 and 70 using yeast or Sso enzymes
SED-ML:
https://jjj.bio.vu.nl/models/experiments/kouril2017_fig4b/simulate
https://jjj.bio.vu.nl/models/experiments/kouril2017_fig4c/simulate
Person responsible: Theresa Kouril
Snapshots: No snapshots
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Assays: PGK - GAPDH models, PGK-GAPDH 30, PGK-GAPDH 70
PGK reaction at 30 C. Yeast PGK was incubated at 30 C, in the presence or absence of the ATP recycling system, and the conversion of 3 PG to BPG was followed.
SED-ML simulations
Fig. 1A: https://jjj.bio.vu.nl/models/experiments/kouril2017_fig1a/simulate
Person responsible: Jacky Snoep
Snapshots: No snapshots
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Assays: PGK 30C data, PGK 30C model
PGK reaction at 70C. Sulfolobus solfataricus PGK was incubated at 70C in presence and absence of an ATP recycling system.
Changes in metabolite concentrations was followed via 31P NMR or enzymatic analyses.
SED-ML:
https://jjj.bio.vu.nl/models/experiments/kouril2017_fig3b/simulate
https://jjj.bio.vu.nl/models/experiments/kouril2017_fig3c/simulate
https://jjj.bio.vu.nl/models/experiments/kouril2017_fig3d/simulate
Person responsible: Theresa Kouril
Snapshots: No snapshots
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Assays: PGK 70 data, PGK 70C model
BPG produced with yeast PGK was incubated at 70 C,upon which BPG rapidly dephosphorylates to 3PG.
SED-ML:
https://jjj.bio.vu.nl/models/experiments/kouril2017_fig2b/simulate
Person responsible: Jacky Snoep
Snapshots: No snapshots
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Assays: BPG degradation, BPG stability analysis
Experimental data for the yeast PGK incubations at 30C, with and without recycling of ATP.
Submitter: Jacky Snoep
Assay type: Metabolite Concentration
Technology type: Technology Type
Snapshots: No snapshots
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Study: PGK-30C
Organisms: No organisms
SOPs: No SOPs
Data files: PGK yeast with recycling of ATP, PGK yeast without recycling of ATP
Changes in metabolite concentrations were either quantified via 31P NMR or enzymatically
Submitter: Theresa Kouril
Assay type: Experimental Assay Type
Technology type: NMR
Snapshots: No snapshots
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Study: PGK-70C
Organisms: No organisms
SOPs: No SOPs
Data files: Sso PGK (14 ug/ml) in presence of recycling sys..., Sso PGK (28 ug/ml) in presence of recycling sys..., Sso PGK in absence of recycling system, 31P NMR..., Sso PGK in presence of ATP recycling system at ...
BPG degradation at 70C
Submitter: Jacky Snoep
Assay type: Metabolite Concentration
Technology type: Technology Type
Snapshots: No snapshots
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Study: BPG stability
Organisms: No organisms
SOPs: No SOPs
Data files: BPG degradation at 70
Submitter: Theresa Kouril
Assay type: Experimental Assay Type
Technology type: Technology Type
Snapshots: No snapshots
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Study: PGK-GAPDH 30C & 70C
Organisms: No organisms
SOPs: No SOPs
Data files: PGK-GAPDH 30, 3PG, GAP & BPG, PGK-GAPDH 30, PYR
Submitter: Theresa Kouril
Assay type: Experimental Assay Type
Technology type: Technology Type
Snapshots: No snapshots
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Study: PGK-GAPDH 30C & 70C
Organisms: No organisms
SOPs: No SOPs
Data files: PGK-GAPDH 70
Submitter: Theresa Kouril
Assay type: Experimental Assay Type
Technology type: Enzymatic Activity Measurements
Snapshots: No snapshots
Investigation: 1 hidden item
Study: 1 hidden item
Organisms: No organisms
SOPs: No SOPs
Data files: non-enzymatic phosphoenolpyruvate (PEP) degrada...
Submitter: Theresa Kouril
Assay type: RNA-seq Profiling
Technology type: Next generation sequencing
Snapshots: No snapshots
Investigation: L-fucose degradation in Sulfolobus solfataricus P2
Study: Comparison of S. solfataricus grown on l-fucose...
Organisms: Sulfolobus solfataricus (batch)
SOPs: SulfoSYS_BIOTECH: RNA isolation and sequencing
Data files: Transcriptomics L-fuc vs D-glc
Submitter: Theresa Kouril
Assay type: Metabolite Profiling
Technology type: Gas Chromatography Mass Spectrometry
Snapshots: No snapshots
Investigation: L-fucose degradation in Sulfolobus solfataricus P2
Study: Comparison of S. solfataricus grown on l-fucose...
Organisms: Sulfolobus solfataricus (batch)
SOPs: No SOPs
Data files: Metabolomics L-fuc vs D-glc
Submitter: Theresa Kouril
Assay type: Proteomics
Technology type: Tandem Mass Spectrometry
Snapshots: No snapshots
Investigation: L-fucose degradation in Sulfolobus solfataricus P2
Study: Comparison of S. solfataricus grown on l-fucose...
Organisms: Sulfolobus solfataricus (batch)
SOPs: SULFOSYSbiotech_Proteome analysis
Data files: Proteomics L-fuc vs D-glc
Submitter: Jacqueline Wolf
Assay type: Experimental Assay Type
Technology type: Enzymatic Activity Measurements
Snapshots: No snapshots
Investigation: L-fucose degradation in Sulfolobus solfataricus P2
Study: Comparison of S. solfataricus grown on l-fucose...
Organisms: Sulfolobus solfataricus (batch)
SOPs: No SOPs
Data files: Activity assay L-fuc vs D-glc
Creators: Theresa Kouril, Jacky Snoep
Submitters: Jacky Snoep, Theresa Kouril
Investigations: Phosphoglycerate kinase acts as a futile cycle ...
Studies: PGK-70C
Assays: PGK 70 data
Experimental data for 3PG conversion to fructose-6-phosphase in reconstituted systems of gluconeogenesis of S. solfataricus
Creators: Jacky Snoep, Theresa Kouril
Submitter: Jacky Snoep
Simulation results of TPI experimental data for GAP and DHAP saturation.
Creators: Jacky Snoep, Theresa Kouril
Submitter: Jacky Snoep
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Model Gluconeogenesis
Assays: TPI Modelling
Simulation results of temperature degradation of gluconeogenic intermediates
Creators: Jacky Snoep, Theresa Kouril
Submitter: Jacky Snoep
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Model Gluconeogenesis
Simulation results of experimental data of the reconstituted gluconeogenic system
Creators: Jacky Snoep, Theresa Kouril
Submitter: Jacky Snoep
Simulation results of GAPDH experimental data for BPG, NADPH, NADP, GAP, and Pi
Creators: Jacky Snoep, Theresa Kouril
Submitter: Jacky Snoep
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Model Gluconeogenesis
Assays: GAPDH Modelling
Creators: Theresa Kouril, Jacky Snoep
Submitter: Theresa Kouril
Investigations: Phosphoglycerate kinase acts as a futile cycle ...
Studies: PGK-70C
Assays: PGK 70 data
Creator: Theresa Kouril
Submitter: Theresa Kouril
Investigations: Phosphoglycerate kinase acts as a futile cycle ...
Studies: PGK-70C
Assays: PGK 70 data
incubations with yeast enzymes at 30C
Creators: Theresa Kouril, Jacky Snoep
Submitter: Theresa Kouril
Investigations: Phosphoglycerate kinase acts as a futile cycle ...
Studies: PGK-GAPDH 30C & 70C
Assays: PGK-GAPDH 30
Creators: Theresa Kouril, Jacky Snoep
Submitter: Theresa Kouril
Investigations: Phosphoglycerate kinase acts as a futile cycle ...
Studies: PGK-GAPDH 30C & 70C
Assays: PGK-GAPDH 30
Creators: Theresa Kouril, Jacky Snoep
Submitter: Theresa Kouril
Investigations: Phosphoglycerate kinase acts as a futile cycle ...
Studies: PGK-GAPDH 30C & 70C
Assays: PGK-GAPDH 70
metabolite quantification via enzymatic analysis
Creators: Theresa Kouril, Jacky Snoep
Submitter: Theresa Kouril
Investigations: Phosphoglycerate kinase acts as a futile cycle ...
Studies: PGK-70C
Assays: PGK 70 data
Creators: Theresa Kouril, Jacky Snoep
Submitter: Theresa Kouril
Investigations: Phosphoglycerate kinase acts as a futile cycle ...
Studies: BPG stability
Assays: BPG degradation
PGK yeast without recycling of ATP
Creators: Jacky Snoep, Theresa Kouril
Submitter: Jacky Snoep
Investigations: Phosphoglycerate kinase acts as a futile cycle ...
Studies: PGK-30C
Assays: PGK 30C data
Creator: Theresa Kouril
Submitter: Theresa Kouril
Investigations: 1 hidden item
Studies: 1 hidden item
RNAseq analysis of S. solfataricus grown on either L-fucose or D-glucose
Creators: Jacqueline Wolf, Theresa Kouril, Andreas Albersmeier, Jörn Kalinowski
Submitter: Jacqueline Wolf
Investigations: L-fucose degradation in Sulfolobus solfataricus P2
Studies: Comparison of S. solfataricus grown on l-fucose...
Assays: RNA sequencing:l-fuc/d-glu
Activity assays in cell free extracts of S. solfataricus grown on either L-fucose or D-glucose and activity assays with recombinant proteins
Creators: Jacqueline Wolf, Katharina Kruse, Bettina Siebers, Theresa Kouril
Submitter: Jacqueline Wolf
Creator: Theresa Kouril
Submitter: Theresa Kouril
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Kinetic characterisation of FBPAase. Expermental data for enzyme reaction rates with increasing concentrations of DHAP and GAP.
Creators: Jacky Snoep, Theresa Kouril
Submitter: Jacky Snoep
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Model Gluconeogenesis
Assays: FBPAase, FBPAase Modelling
Kinetic characterisation of TPI. Experimental data for enzyme reaction rates with increasing concentrations of DHAP and GAP, and inhibition with 3PG and PEP.
Creators: Jacky Snoep, Theresa Kouril
Submitter: Jacky Snoep
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Model Gluconeogenesis
Assays: TPI, TPI Modelling
Creators: Theresa Kouril, Andreas Albersmeier, CeBiTech, University Bielefeld, Germany; Jörn Kalinowski, CeBiTech, University Bielefeld;
Submitter: Theresa Kouril
Investigations: Amino acid degradation in Sulfolobus solfataric..., L-fucose degradation in Sulfolobus solfataricus P2
Studies: Comparison of S. solfataricus grown on l-fucose..., Comparison of Sulfolobus solfataricus P2 grown ...
Assays: RNA sequencing: Casaminoacids vs D-glc, RNA sequencing:l-fuc/d-glu
Protein extraction, iTRAQ labeling, peptides separation, mass spectrometry and data analysis
Creators: Theresa Kouril, Phil Wright, Trong Khoa Pham
Submitter: Theresa Kouril
Investigations: Amino acid degradation in Sulfolobus solfataric..., L-fucose degradation in Sulfolobus solfataricus P2
Studies: Comparison of S. solfataricus grown on l-fucose..., Comparison of Sulfolobus solfataricus P2 grown ...
Assays: Proteome analysis: Casaminoacids versus D-Glc, Proteome analysis: d-fuc / l-glu

Creator: Theresa Kouril
Submitter: Theresa Kouril
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Preparation of cell free extracts of the recombinant E. coli strains expressing the gluconeogenic S. solfataricus enzymes.
Creators: Jacky Snoep, Theresa Kouril
Submitter: Jacky Snoep
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Model Gluconeogenesis
Cloning and heterologous expression of gluconeogenic enzymes from S. solfataricus in E. coli
Creators: Jacky Snoep, Theresa Kouril
Submitter: Jacky Snoep
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Model Gluconeogenesis
Purification of gluconeogenic enzymes from S. solfataricus in recombinant E.coli extracts
Creators: Jacky Snoep, Theresa Kouril
Submitter: Jacky Snoep
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Model Gluconeogenesis
Abstract (Expand)
Authors: P. Haferkamp, B. Tjaden, L. Shen, C. Brasen, T. Kouril, B. Siebers
Date Published: 30th Apr 2019
Publication Type: Journal
PubMed ID: 31031731
Citation: Front Microbiol. 2019 Apr 12;10:757. doi: 10.3389/fmicb.2019.00757. eCollection 2019.
Abstract (Expand)
Authors: T. Kouril, J. J. Eicher, B. Siebers, J. L. Snoep
Date Published: 7th Oct 2017
Publication Type: Not specified
PubMed ID: 28982396
Citation: Microbiology. 2017 Nov;163(11):1604-1612. doi: 10.1099/mic.0.000542. Epub 2017 Oct 6.
Abstract (Expand)
Authors: A. S. Figueiredo, T. Kouril, D. Esser, P. Haferkamp, P. Wieloch, D. Schomburg, P. Ruoff, B. Siebers, J. Schaber
Date Published: 12th Jul 2017
Publication Type: Not specified
PubMed ID: 28692669
Citation: PLoS One. 2017 Jul 10;12(7):e0180331. doi: 10.1371/journal.pone.0180331. eCollection 2017.
Abstract (Expand)
Authors: J. Wolf, H. Stark, K. Fafenrot, A. Albersmeier, T. K. Pham, K. B. Muller, B. Meyer, L. Hoffmann, L. Shen, S. P. Albaum, T. Kouril, K. Schmidt-Hohagen, M. Neumann-Schaal, C. Brasen, J. Kalinowski, P. C. Wright, S. V. Albers, D. Schomburg, B. Siebers
Date Published: 10th Sep 2016
Publication Type: Not specified
PubMed ID: 27611014
Citation: Mol Microbiol. 2016 Sep 9. doi: 10.1111/mmi.13498.
Abstract (Expand)
Authors: , Dominik Esser, Julia Kort, , ,
Date Published: 20th Jul 2013
Publication Type: Not specified
PubMed ID: 23865479
Citation:
All contributors
Abstract (Expand)
Authors: , Dominik Esser, , , , , , Julia Reimann, , , Daniela Teichmann, Marleen van Wolferen, , , , , , , , , ,
Date Published: 31st Aug 2009
Publication Type: Not specified
PubMed ID: 19802714
Citation: