SOPs

What is a SOP?

10 SOPs visible to you, out of a total of 10

SOP_Preparation of cell lysates

Glucose metabolism in cancer cell lines

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: Metabolic control analysis of glyceraldehyde 3-...

Studies: 3-Bromopyruvate (3BrP) titrations, Iodoacetic acid (IAA) titrations in cancer cell...

Assays: GAPDH and flux inhibition in MCF7 and MDA231cells, HK, GAPDH and flux inhibition in MDA231 cells, Incubation time for inhibitor treatment, Target verification/ Specificity of 3BrP in gly...

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Created: 13th Jul 2023 at 12:52

SOP_Inhibitor titration intact cells

Glucose metabolism in cancer cell lines

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: Metabolic control analysis of glyceraldehyde 3-...

Studies: 3-Bromopyruvate (3BrP) titrations, Iodoacetic acid (IAA) titrations in cancer cell...

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Created: 13th Jul 2023 at 12:49

SOP_Quantification of glycolytic flux (glucose, lactate)

Glucose metabolism in cancer cell lines

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: Metabolic control analysis of glyceraldehyde 3-...

Studies: 3-Bromopyruvate (3BrP) titrations, Iodoacetic acid (IAA) titrations in cancer cell...

Assays: GAPDH and flux inhibition in MCF7 and MDA231cells, HK, GAPDH and flux inhibition in MDA231 cells, Incubation time for inhibitor treatment

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Created: 12th Jul 2023 at 13:05

SOP_Cancer cell lines_standard cell culture

Glucose metabolism in cancer cell lines

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: Metabolic control analysis of glyceraldehyde 3-...

Studies: 3-Bromopyruvate (3BrP) titrations, Iodoacetic acid (IAA) titrations in cancer cell...

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Created: 12th Jul 2023 at 13:03

SulfoSYS_BIOTECH: RNA isolation and sequencing

SulfoSys - Biotec

No description specified

Creators: Theresa Kouril, Andreas Albersmeier, CeBiTech, University Bielefeld, Germany; Jörn Kalinowski, CeBiTech, University Bielefeld;

Submitter: Theresa Kouril

Investigations: Amino acid degradation in Sulfolobus solfataric..., L-fucose degradation in Sulfolobus solfataricus P2

Studies: Comparison of S. solfataricus grown on l-fucose..., Comparison of Sulfolobus solfataricus P2 grown ...

Assays: RNA sequencing: Casaminoacids vs D-glc, RNA sequencing:l-fuc/d-glu

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Created: 17th Dec 2014 at 12:45, Last updated: 20th Oct 2016 at 06:23

SULFOSYSbiotech_Proteome analysis

SulfoSys - Biotec

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Protein extraction, iTRAQ labeling, peptides separation, mass spectrometry and data analysis

Creators: Theresa Kouril, Phil Wright, Trong Khoa Pham

Submitter: Theresa Kouril

Investigations: Amino acid degradation in Sulfolobus solfataric..., L-fucose degradation in Sulfolobus solfataricus P2

Studies: Comparison of S. solfataricus grown on l-fucose..., Comparison of Sulfolobus solfataricus P2 grown ...

Assays: Proteome analysis: Casaminoacids versus D-Glc, Proteome analysis: d-fuc / l-glu

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Created: 5th Feb 2015 at 12:45, Last updated: 20th Oct 2016 at 06:23

SULFOSYSbiotech_Protein extraction_iTRAQ labeling_Peptides separation_mass spectrometry_data analysis

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

Created: 5th Feb 2015 at 12:14

Preparation of cell extracts of recombinant E.coli

SulfoSys

Preparation of cell free extracts of the recombinant E. coli strains expressing the gluconeogenic S. solfataricus enzymes.

Creators: Jacky Snoep, Theresa Kouril

Submitter: Jacky Snoep

Investigations: Central Carbon Metabolism of Sulfolobus solfata...

Studies: Model Gluconeogenesis

Assays: FBPAase, GAPDH, PGK, TPI

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Created: 1st Aug 2013 at 09:26, Last updated: 1st Aug 2013 at 14:29

Cloning and heterologous expression in E. coli

SulfoSys

Cloning and heterologous expression of gluconeogenic enzymes from S. solfataricus in E. coli

Creators: Jacky Snoep, Theresa Kouril

Submitter: Jacky Snoep

Investigations: Central Carbon Metabolism of Sulfolobus solfata...

Studies: Model Gluconeogenesis

Assays: FBPAase, GAPDH, PGK, TPI

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Created: 1st Aug 2013 at 09:21, Last updated: 1st Aug 2013 at 14:28

Protein purification from E. coli extracts

SulfoSys

Purification of gluconeogenic enzymes from S. solfataricus in recombinant E.coli extracts

Creators: Jacky Snoep, Theresa Kouril

Submitter: Jacky Snoep

Investigations: Central Carbon Metabolism of Sulfolobus solfata...

Studies: Model Gluconeogenesis

Assays: FBPAase, GAPDH, PGK, TPI

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Created: 1st Aug 2013 at 09:23, Last updated: 1st Aug 2013 at 14:28

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