Proteome analysis: Casaminoacids versus D-Glc
SEEK ID: https://fairdomhub.org/assays/402
Experimental Assay
Projects: SulfoSys - Biotec
Investigation: Amino acid degradation in Sulfolobus solfataricus P2
Study: Comparison of Sulfolobus solfataricus P2 grown on caseinhydrolysate and D-glucose
Assay position:
Assay type: Proteomics
Technology type: Tandem Mass Spectrometry
Organisms: Sulfolobus solfataricus (batch)
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Created: 7th Aug 2016 at 18:28
Last updated: 18th Oct 2018 at 11:38
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Projects: SulfoSys, SulfoSys - Biotec
Institutions: University of Sheffield
https://orcid.org/0000-0002-9671-5398 
Projects: SulfoSys, SulfoSys - Biotec
Institutions: University of Sheffield
I am the foundation Professor of Systems Biology and Engineering within the Department of Chemical and Process Engineering (CPE), at The University of Sheffield. My research philosophy is centred on a mechanistic systems biology approach to solve biochemical reaction engineered processes. I wish to pursue issues involved in the effective utilisation of biological resources. The approach is specifically targeted at the conjunction of chemical engineering (metabolic engineering and synthetic biology), ...
e:Bio - Innovations Competition Systems Biology
Projects: SulfoSys - Biotec, SBEpo - Systems Biology of Erythropoietin
Web page: http://www.fona.de/en/14276
Within the e:Bio - Innovationswettbewerb Systembiologie (Federal Ministry of Education and Research (BMBF)), the SulfoSYSBIOTECH consortium (10 partners), aim to unravel the complexity and regulation of the carbon metabolic network of the thermoacidophilic archaeon Sulfolobus solfataricus (optimal growth at 80°C and pH 3) in order to provide new catalysts ‘extremozymes’ for utilization in White Biotechnology.
Based on the available S. solfataricus genome scale metabolic model (Ulas et al., 2012) ...
Programme: e:Bio
Public web page: http://www.sulfosys.com/
Integrated systems biology approach including transcriptome, metabolome, proteome analyses and modelling to elucidate amino acid degradation in S. solfataricus P2.
Submitter: Jacqueline Wolf
Studies: Comparison of Sulfolobus solfataricus P2 grown on caseinhydrolysate and ...
Assays: Metabolic modelling of S. solfataricus during growth on casaminoacids, Metabolome analysis: Casaminoacids versus D-Glc, Proteome analysis: Casaminoacids versus D-Glc, RNA sequencing: Casaminoacids vs D-glc
To investigate amino acid degradation pathways in Sulfolobus solfataricus transcriptome, proteome and metabolome analyses were performed on cells grown on caseinhydrolysate as carbon source. Cells grown with glucose served as reference condition. Metabolic modelling was used to compare the efficiency of different degradation routes.
Submitter: Jacqueline Wolf
Investigation: Amino acid degradation in Sulfolobus solfataric...
Assays: Metabolic modelling of S. solfataricus during growth on casaminoacids, Metabolome analysis: Casaminoacids versus D-Glc, Proteome analysis: Casaminoacids versus D-Glc, RNA sequencing: Casaminoacids vs D-glc
Supplementary file required by main Zip archive file for file extraction.
Creators: Helge Stark, Trong Khoa Pham, Phil Wright
Submitter: Helge Stark
Relationship type: Not specified
Cells of S. solfataricus were grown on either caseinhydrolysate or D-glucose (reference) as sole carbon source.
These files contain the output results from Phenyx searching, providing all necessary information for matching MS data with protein database.
Creators: Jacqueline Wolf, Trong Khoa Pham, Phil Wright
Submitter: Jacqueline Wolf
Relationship type: Not specified
This file contains the results from proteome analysis of Sulfolobus solfataricus grown on Caseinhydrolysate as carbon source (growth on D-glucose served as reference condition).
Creators: Jacqueline Wolf, Trong Khoa Pham, Phil Wright
Submitter: Jacqueline Wolf
Relationship type: Not specified
Raw MS data files of the comparison of Sulfolobus solfataricus grown on either caseinhydrolysate or D-glucose. The numbers represents the fractions collected from the HPLC run (e.g. 22 indicates that this sample was collected from 21 min to 22 min). For MS analysis every two fractions were combined, indicated by double numbers (e.g. 80-81: combined fractions 80 and 81). Furhter all combined fractions were run twice on the MS. Zip archive. Requires the supplementary .z01, .z02 and .z03 files for ...
Creators: Helge Stark, Trong Khoa Pham, Phil Wright
Submitter: Helge Stark
Relationship type: Not specified
Supplementary file required by main Zip archive file for file extraction.
Creators: Helge Stark, Trong Khoa Pham, Phil Wright
Submitter: Helge Stark
Relationship type: Not specified
Supplementary file required by main Zip archive file for file extraction.
Creators: Helge Stark, Trong Khoa Pham, Phil Wright
Submitter: Helge Stark
Relationship type: Not specified
Protein extraction, iTRAQ labeling, peptides separation, mass spectrometry and data analysis
Creators: Theresa Kouril, Phil Wright, Trong Khoa Pham
Submitter: Theresa Kouril
