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PGK-GAPDH 30, 3PG, GAP & BPG

PGK-GAPDH 30, 3PG, GAP & BPG
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incubations with yeast enzymes at 30C

SEEK ID: https://fairdomhub.org/data_files/1972?version=1

Filename: Fig4b-data-gap-3pg-bpg R .xls

Format: Spreadsheet

Size: 81.5 KB

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Unexpand

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Views: 1756 Downloads: 90

Created: 28th Sep 2017 at 13:56

Last updated: 28th Sep 2017 at 14:07

Last used: 18th Nov 2019 at 04:04

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Investigations (1)
Studies (1)
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Theresa Kouril

Projects: SulfoSys, SulfoSys - Biotec

Institutions: University Duisburg-Essen

Roles: PhD Student, Postdoc

Expertise: Glycolysis, Systems Biology, Microbiology, functional protein expression, Molecular Biology, Carbohydrate Metabolism of Archaea, carbon metabolism, Biochemistry

Tools: Enzyme assays, Molecular Biology, Biochemistry

Jacky Snoep

Projects: PSYSMO, MOSES, SysMO DB, SysMO-LAB, SulfoSys, SulfoSys - Biotec, Whole body modelling of glucose metabolism in malaria patients, FAIRDOM, Molecular Systems Biology, COMBINE Multicellular Modelling, HOTSOLUTE, Steroid biosynthesis, Yeast glycolytic oscillations, Computational pathway design for biotechnological applications

Institutions: Manchester Centre for Integrative Systems Biology, University of Manchester, University of Stellenbosch, School of Computer Science, University of Manchester, Stellenbosch University

SulfoSys

Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation

Programme: SysMO

Public web page: http://sulfosys.com/

Organisms: Sulfolobus solfataricus

Phosphoglycerate kinase acts as a futile cycle at high temperature

SulfoSys, SulfoSys - Biotec

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Jacky Snoep

Theresa Kouril

Dawie Van Niekerk

The gluconeogenic conversion of 3-phosphoglycerate via 1,3-bisphosphoglycerate to glyceraldehyde-3-phosphate was compared at 30 C and at 70 C. At 30 C it was possible to produce 1,3-bisphosphoglycerate from 3-phosphoglycerate with phosphoglycerate kinase, but at 70 C, 1,3- bisphosphoglycerate was dephosphorylated rapidly to 3-phosphoglycerate, effectively turning the phosphoglycerate kinase into a futile cycle. At both temperatures it was possible to convert 3-phosphoglycerate to glyceraldehyde
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Studies: BPG stability, PGK-30C, PGK-70C, PGK-GAPDH 30C & 70C

Assays: BPG degradation, BPG stability analysis, PGK - GAPDH models, PGK 30C data, PGK 30C model, PGK 70 data, PGK 70C model, PGK-GAPDH 30, PGK-GAPDH 70