PGK 70 data
Changes in metabolite concentrations were either quantified via 31P NMR or enzymatically
SEEK ID: https://fairdomhub.org/assays/571
Experimental assay
Projects: SulfoSys, SulfoSys - Biotec
Investigation: Phosphoglycerate kinase acts as a futile cycle at high temperature
Study: PGK-70C
Assay position:
Assay type: Experimental Assay Type
Technology type: NMR
Organisms: No organisms
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Created: 28th Sep 2017 at 11:16
Last updated: 8th Nov 2017 at 14:21
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Institutions: Manchester Centre for Integrative Systems Biology, University of Manchester, University of Stellenbosch, University of Manchester - Department of Computer Science, Stellenbosch University
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
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Web page: http://www.fona.de/en/14276
Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation
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Public web page: http://sulfosys.com/
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The gluconeogenic conversion of 3-phosphoglycerate via 1,3-bisphosphoglycerate to glyceraldehyde-3-phosphate was compared at 30 C and at 70 C. At 30 C it was possible to produce 1,3-bisphosphoglycerate from 3-phosphoglycerate with phosphoglycerate kinase, but at 70 C, 1,3- bisphosphoglycerate was dephosphorylated rapidly to 3-phosphoglycerate, effectively turning the phosphoglycerate kinase into a futile cycle. At both temperatures it was possible to convert 3-phosphoglycerate to glyceraldehyde ...
Submitter: Jacky Snoep
Studies: BPG stability, PGK-30C, PGK-70C, PGK-GAPDH 30C & 70C
Assays: BPG degradation, BPG stability analysis, PGK - GAPDH models, PGK 30C data, PGK 30C model, PGK 70 data, PGK 70C model, PGK-GAPDH 30, PGK-GAPDH 70
PGK reaction at 70C. Sulfolobus solfataricus PGK was incubated at 70C in presence and absence of an ATP recycling system. Changes in metabolite concentrations was followed via 31P NMR or enzymatic analyses.
SED-ML: https://jjj.bio.vu.nl/models/experiments/kouril2017_fig3b/simulate https://jjj.bio.vu.nl/models/experiments/kouril2017_fig3c/simulate https://jjj.bio.vu.nl/models/experiments/kouril2017_fig3d/simulate
Submitter: Theresa Kouril
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Assays: PGK 70 data, PGK 70C model
Creators: Theresa Kouril, Jacky Snoep
Submitters: Jacky Snoep, Theresa Kouril
Relationship type: Not specified
metabolite quantification via enzymatic analysis
