PGK 30C data
Experimental data for the yeast PGK incubations at 30C, with and without recycling of ATP.
SEEK ID: https://fairdomhub.org/assays/569
Experimental assay
Projects: SulfoSys, SulfoSys - Biotec
Investigation: Phosphoglycerate kinase acts as a futile cycle at high temperature
Study: PGK-30C
Assay position:
Assay type: Metabolite Concentration
Technology type: Technology Type
Organisms: No organisms
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Created: 28th Sep 2017 at 10:08
Last updated: 8th Nov 2017 at 14:21
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The gluconeogenic conversion of 3-phosphoglycerate via 1,3-bisphosphoglycerate to glyceraldehyde-3-phosphate was compared at 30 C and at 70 C. At 30 C it was possible to produce 1,3-bisphosphoglycerate from 3-phosphoglycerate with phosphoglycerate kinase, but at 70 C, 1,3- bisphosphoglycerate was dephosphorylated rapidly to 3-phosphoglycerate, effectively turning the phosphoglycerate kinase into a futile cycle. At both temperatures it was possible to convert 3-phosphoglycerate to glyceraldehyde ...
Submitter: Jacky Snoep
Studies: BPG stability, PGK-30C, PGK-70C, PGK-GAPDH 30C & 70C
Assays: BPG degradation, BPG stability analysis, PGK - GAPDH models, PGK 30C data, PGK 30C model, PGK 70 data, PGK 70C model, PGK-GAPDH 30, PGK-GAPDH 70
PGK reaction at 30 C. Yeast PGK was incubated at 30 C, in the presence or absence of the ATP recycling system, and the conversion of 3 PG to BPG was followed. SED-ML simulations Fig. 1A: https://jjj.bio.vu.nl/models/experiments/kouril2017_fig1a/simulate
Submitter: Jacky Snoep
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Assays: PGK 30C data, PGK 30C model
PGK yeast without recycling of ATP
PGK yeast with recycling of ATP
