PGK-GAPDH 30C & 70C

Actions

Order Assays

Overview
Related items

PGK-GAPDH reactions were studied in vitro at 30 and 70 using yeast or Sso enzymes

SED-ML: https://jjj.bio.vu.nl/models/experiments/kouril2017_fig4b/simulate https://jjj.bio.vu.nl/models/experiments/kouril2017_fig4c/simulate

SEEK ID: https://fairdomhub.org/studies/290

Investigation: Phosphoglycerate kinase acts as a futile cycle at high temperature

Projects: SulfoSys, SulfoSys - Biotec

Study position:

Experimentalists: Theresa Kouril Jacky Snoep

Help

Tree Split Graph

Export PNG

Click below to download a copy of the image

Download

Creators and Submitter

Creators

Theresa Kouril
Jacky Snoep

Submitter

Theresa Kouril

Activity

Views: 2494

Created: 28th Sep 2017 at 11:41

Last updated: 28th Sep 2017 at 13:11

Related items

People (2)
Programmes (2)
Projects (2)
Investigations (1)
Assays (3)
Data files (3)
Models (3)

Advanced People list for this Study with search and filtering

Theresa Kouril

Projects: SulfoSys, SulfoSys - Biotec, Glucose metabolism in cancer cell lines

Institutions: University Duisburg-Essen, Stellenbosch University

Expertise: Biochemistry, carbon metabolism, Carbohydrate Metabolism of Archaea, functional protein expression, Glycolysis, Microbiology, Molecular Biology, Systems Biology

Tools: Biochemistry, Molecular Biology, Enzyme assays

Jacky Snoep

Projects: PSYSMO, MOSES, SysMO DB, SysMO-LAB, SulfoSys, SulfoSys - Biotec, Whole body modelling of glucose metabolism in malaria patients, FAIRDOM, Molecular Systems Biology, COMBINE Multicellular Modelling, HOTSOLUTE, Steroid biosynthesis, Yeast glycolytic oscillations, Computational pathway design for biotechnological applications, SCyCode The Autotrophy-Heterotrophy Switch in Cyanobacteria: Coherent Decision-Making at Multiple Regulatory Layers, Project Coordination, WP 3: Drug release kinetics study, Glucose metabolism in cancer cell lines

Institutions: Manchester Centre for Integrative Systems Biology, University of Manchester, University of Stellenbosch, University of Manchester - Department of Computer Science, Stellenbosch University

Advanced Programmes list for this Study with search and filtering

SysMO

SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".

The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.

Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...

Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep

Web page: http://sysmo.net/

e:Bio

e:Bio - Innovations Competition Systems Biology

Projects: SulfoSys - Biotec, SBEpo - Systems Biology of Erythropoietin

Web page: http://www.fona.de/en/14276

Advanced Projects list for this Study with search and filtering

SulfoSys

Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation

Programme: SysMO

Public web page: http://sulfosys.com/

Organisms: Sulfolobus solfataricus

SulfoSys - Biotec

Within the e:Bio - Innovationswettbewerb Systembiologie (Federal Ministry of Education and Research (BMBF)), the SulfoSYSBIOTECH consortium (10 partners), aim to unravel the complexity and regulation of the carbon metabolic network of the thermoacidophilic archaeon Sulfolobus solfataricus (optimal growth at 80°C and pH 3) in order to provide new catalysts ‘extremozymes’ for utilization in White Biotechnology.

Based on the available S. solfataricus genome scale metabolic model (Ulas et al., 2012) ...

Programme: e:Bio

Public web page: http://www.sulfosys.com/

Organisms: Sulfolobus solfataricus

Advanced Investigations list for this Study with search and filtering

Phosphoglycerate kinase acts as a futile cycle at high temperature

SulfoSys, SulfoSys - Biotec

(Show All)

All creators

Jacky Snoep

Theresa Kouril

Dawie van Niekerk

The gluconeogenic conversion of 3-phosphoglycerate via 1,3-bisphosphoglycerate to glyceraldehyde-3-phosphate was compared at 30 C and at 70 C. At 30 C it was possible to produce 1,3-bisphosphoglycerate from 3-phosphoglycerate with phosphoglycerate kinase, but at 70 C, 1,3- bisphosphoglycerate was dephosphorylated rapidly to 3-phosphoglycerate, effectively turning the phosphoglycerate kinase into a futile cycle. At both temperatures it was possible to convert 3-phosphoglycerate to glyceraldehyde ...

Submitter: Jacky Snoep

Studies: BPG stability, PGK-30C, PGK-70C, PGK-GAPDH 30C & 70C

Assays: BPG degradation, BPG stability analysis, PGK - GAPDH models, PGK 30C data, PGK 30C model, PGK 70 data, PGK 70C model, PGK-GAPDH 30, PGK-GAPDH 70

Snapshots: No snapshots

Created: 27th Sep 2017 at 12:36

Advanced Assays list for this Study with search and filtering