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| Metadata | Values (examples) | ||||||||
| Asset Title | PGK-GAPDH reaction 30C | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | SulfoSys | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | |||||||||
| Assay Title | yeast PGK-GAPDH reaction | ||||||||
| Assay_type | Metabolite concentration | ||||||||
| Technology_type | progressive curve experiment | ||||||||
| Description | Progress curve of yeast Phosphoglycerate kinase-Glyceraldehyde 3-phosphate couple. The conversion of 3PG to GAP was followed over time | ||||||||
| Experimentalist | Theresa Kouril | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | concentration | concentration | concentration | ||||||
| Compound (if concentration) | 3PG | GAP | BPG | ||||||
| Unit | mM | mM | mM | ||||||
| Start_value (optional) | 3 | 0 | 0 | ||||||
| End_value (optional) | 0 | 3 | 0 | ||||||
| Comments | |||||||||
| Culture growth | |||||||||
| FACTORS_STUDIED | |||||||||
| Item | enzymeActivity | ||||||||
| Compound (if concentration) | yeast PGK-GAPDH activity at 30C | ||||||||
| Unit | ug/ml | ||||||||
| Start_value (optional) | |||||||||
| End_value (optional) | |||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | Phosphoglycerate Kinase | Glyceraldehyde 3-phosphate | |||||||
| EC Number | 2.7.2.3 | 1.2.1.12 | |||||||
| Sequence Accession Number (uniprot) | P50317 | ||||||||
| Reaction | KEGG | CHEBI | |||||||
| 3PG | C00197 | 17794 | |||||||
| GAP | C00661 | 17138 | |||||||
| BPG | C00236 | 28907 | |||||||
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| time | GAP | stdev | 3PG | stdev | BPG | stdev | |||
| 0 | 0.365497076 | 0.044808451 | 3.11754386 | 0.114196916 | 0.149122807 | 0.136459203 | |||
| 40 | 1.306432749 | 0.216695668 | 1.735087719 | 0.222350686 | 0.139912281 | 0.122193014 | |||
| 80 | 2.373099415 | 0.267969654 | 0.740350877 | 0.309175374 | 0.136052632 | 0.105321694 | |||
| 120 | 3.195906433 | 0.154289577 | 0.263157895 | 0.199591766 | 0.121929825 | 0.050862067 | |||
