SEEK ID: https://fairdomhub.org/assays/310
Experimental assay
Projects: SulfoSys - Biotec
Investigation: L-fucose degradation in Sulfolobus solfataricus P2
Study: Comparison of S. solfataricus grown on l-fucose and d-glucose
Assay position:
Assay type: Experimental Assay Type
Technology type: Enzymatic Activity Measurements
Organisms: Sulfolobus solfataricus
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Created: 3rd Dec 2015 at 07:24
Last updated: 8th Nov 2017 at 14:21
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Institutions: University Duisburg-Essen, Stellenbosch University
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https://orcid.org/0000-0002-9905-541XHead of the group of Molecular Enzyme Technology and Biochemistry (Faculty of Chemistry) at the University of Duisburg-Essen. My research interest is on archaeal physiology with a special focuss on the central carbohydrate metabolism of (hyper)thermophilic Archaea and its regulation. The aim is to gain a systems level understanding by the combination of modern highthrouput analyses with classical biochemistry and molecular biology. Archaea possess many novel enzymes and pathways and our aim is ...
e:Bio - Innovations Competition Systems Biology
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Web page: http://www.fona.de/en/14276
Within the e:Bio - Innovationswettbewerb Systembiologie (Federal Ministry of Education and Research (BMBF)), the SulfoSYSBIOTECH consortium (10 partners), aim to unravel the complexity and regulation of the carbon metabolic network of the thermoacidophilic archaeon Sulfolobus solfataricus (optimal growth at 80°C and pH 3) in order to provide new catalysts ‘extremozymes’ for utilization in White Biotechnology.
Based on the available S. solfataricus genome scale metabolic model (Ulas et al., 2012) ...
Programme: e:Bio
Public web page: http://www.sulfosys.com/
Organisms: Sulfolobus solfataricus
Integrated systems biology approach including transcriptome, metabolome, biochemistry, proteome analyses and modelling to elucidate the catabolic pathway for L-fucose in S. solfataricus P2.
Submitter: Theresa Kouril
Studies: Comparison of S. solfataricus grown on l-fucose and d-glucose
Assays: Cell free extract activity measurements: L-fuc/d-glc, Metabolic model of Sulfolobus solfataricus, Proteome analysis: d-fuc / l-glu, RNA sequencing:l-fuc/d-glu, intracellular metabolome analysis: l-fucose vs d-glucose
Snapshots: No snapshots
Submitter: Theresa Kouril
Investigation: L-fucose degradation in Sulfolobus solfataricus P2
Assays: Cell free extract activity measurements: L-fuc/d-glc, Metabolic model of Sulfolobus solfataricus, Proteome analysis: d-fuc / l-glu, RNA sequencing:l-fuc/d-glu, intracellular metabolome analysis: l-fucose vs d-glucose
Snapshots: No snapshots
Activity assays in cell free extracts of S. solfataricus grown on either L-fucose or D-glucose and activity assays with recombinant proteins
Creators: Jacqueline Wolf, Katharina Kruse, Bettina Siebers, Theresa Kouril
Submitter: Jacqueline Wolf
Relationship type: Not specified