Assays

What is an Assay?

15 Assays visible to you, out of a total of 16

Incubation time for inhibitor treatment

Glucose metabolism in cancer cell lines

No description specified

Submitter: Theresa Kouril

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Metabolic control analysis of glyceraldehyde 3-...

Study: Iodoacetic acid (IAA) titrations in cancer cell...

Organisms: No organisms

SOPs: SOP_Cancer cell lines_standard cell culture, SOP_Inhibitor titration intact cells, SOP_Preparation of cell lysates, SOP_Quantification of glycolytic flux (glucose,...

Data files: MDA231_IAA_intact cells_time course

Snapshots: No snapshots

Created: 13th Jul 2023 at 12:59, Last updated: 19th Jul 2023 at 10:34

GAPDH and flux inhibition in MCF7 and MDA231cells

Glucose metabolism in cancer cell lines

No description specified

Submitter: Theresa Kouril

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Metabolic control analysis of glyceraldehyde 3-...

Study: Iodoacetic acid (IAA) titrations in cancer cell...

Organisms: Homo sapiens

SOPs: SOP_Cancer cell lines_standard cell culture, SOP_Inhibitor titration intact cells, SOP_Preparation of cell lysates, SOP_Quantification of glycolytic flux (glucose,...

Data files: MCF7_IAA titration_flux_GAPDH_intact cells, MDA231_IAA titration_flux_GAPDH_intact cells

Snapshots: No snapshots

Created: 12th Jul 2023 at 13:18, Last updated: 19th Jul 2023 at 10:19

Target verification/ Specificity of 3BrP in glycolysis

Glucose metabolism in cancer cell lines

No description specified

Submitter: Theresa Kouril

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Metabolic control analysis of glyceraldehyde 3-...

Study: 3-Bromopyruvate (3BrP) titrations

Organisms: No organisms

SOPs: SOP_Cancer cell lines_standard cell culture, SOP_Inhibitor titration intact cells, SOP_Preparation of cell lysates

Data files: MDA231_3BrP_intact cells_specificity

Snapshots: No snapshots

Created: 13th Jul 2023 at 13:46, Last updated: 19th Jul 2023 at 10:17

HK, GAPDH and flux inhibition in MDA231 cells

Glucose metabolism in cancer cell lines

No description specified

Submitter: Theresa Kouril

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Metabolic control analysis of glyceraldehyde 3-...

Study: 3-Bromopyruvate (3BrP) titrations

Organisms: No organisms

SOPs: SOP_Cancer cell lines_standard cell culture, SOP_Inhibitor titration intact cells, SOP_Preparation of cell lysates, SOP_Quantification of glycolytic flux (glucose,...

Data files: MDA231_3BrP__flux_HK_GAPDH_intact cells

Snapshots: No snapshots

Created: 13th Jul 2023 at 13:25, Last updated: 19th Jul 2023 at 10:15

Traget verification/ Specificity of IAA in glycolysis

Glucose metabolism in cancer cell lines

No description specified

Submitter: Theresa Kouril

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Metabolic control analysis of glyceraldehyde 3-...

Study: Iodoacetic acid (IAA) titrations in cancer cell...

Organisms: No organisms

SOPs: No SOPs

Data files: MDA231_IAA_intact cells_specificity

Snapshots: No snapshots

Created: 13th Jul 2023 at 13:08, Last updated: 13th Jul 2023 at 13:55

PGK 30C data

SulfoSys, SulfoSys - Biotec

Experimental data for the yeast PGK incubations at 30C, with and without recycling of ATP.

Submitter: Jacky Snoep

Assay type: Metabolite Concentration

Technology type: Technology Type

Investigation: Phosphoglycerate kinase acts as a futile cycle ...

Study: PGK-30C

Organisms: No organisms

SOPs: No SOPs

Data files: PGK yeast with recycling of ATP, PGK yeast without recycling of ATP

Snapshots: No snapshots

Created: 28th Sep 2017 at 10:08, Last updated: 8th Nov 2017 at 14:21

PGK 70 data

SulfoSys, SulfoSys - Biotec

Changes in metabolite concentrations were either quantified via 31P NMR or enzymatically

Submitter: Theresa Kouril

Assay type: Experimental Assay Type

Technology type: NMR

Investigation: Phosphoglycerate kinase acts as a futile cycle ...

Study: PGK-70C

Organisms: No organisms

SOPs: No SOPs

Data files: Sso PGK (14 ug/ml) in presence of recycling sys..., Sso PGK (28 ug/ml) in presence of recycling sys..., Sso PGK in absence of recycling system, 31P NMR..., Sso PGK in presence of ATP recycling system at ...

Snapshots: No snapshots

Created: 28th Sep 2017 at 11:16, Last updated: 8th Nov 2017 at 14:21

BPG degradation

SulfoSys, SulfoSys - Biotec

BPG degradation at 70C

Submitter: Jacky Snoep

Assay type: Metabolite Concentration

Technology type: Technology Type

Investigation: Phosphoglycerate kinase acts as a futile cycle ...

Study: BPG stability

Organisms: No organisms

SOPs: No SOPs

Data files: BPG degradation at 70

Snapshots: No snapshots

Created: 28th Sep 2017 at 11:17, Last updated: 8th Nov 2017 at 14:21

PGK-GAPDH 30

SulfoSys, SulfoSys - Biotec

No description specified

Submitter: Theresa Kouril

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Phosphoglycerate kinase acts as a futile cycle ...

Study: PGK-GAPDH 30C & 70C

Organisms: No organisms

SOPs: No SOPs

Data files: PGK-GAPDH 30, 3PG, GAP & BPG, PGK-GAPDH 30, PYR

Snapshots: No snapshots

Created: 28th Sep 2017 at 11:58, Last updated: 8th Nov 2017 at 14:21

PGK-GAPDH 70

SulfoSys, SulfoSys - Biotec

No description specified

Submitter: Theresa Kouril

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Phosphoglycerate kinase acts as a futile cycle ...

Study: PGK-GAPDH 30C & 70C

Organisms: No organisms

SOPs: No SOPs

Data files: PGK-GAPDH 70

Snapshots: No snapshots

Created: 28th Sep 2017 at 12:01, Last updated: 8th Nov 2017 at 14:21

non-enzymatic phosphoenolpyruvate (PEP) degradation at high temperature

SulfoSys, SulfoSys - Biotec

No description specified

Submitter: Theresa Kouril

Assay type: Experimental Assay Type

Technology type: Enzymatic Activity Measurements

Investigation: 1 hidden item

Study: 1 hidden item

Organisms: No organisms

SOPs: No SOPs

Data files: non-enzymatic phosphoenolpyruvate (PEP) degrada...

Snapshots: No snapshots

Created: 27th Mar 2017 at 10:14, Last updated: 8th Nov 2017 at 14:21

RNA sequencing:l-fuc/d-glu

SulfoSys - Biotec

No description specified

Submitter: Theresa Kouril

Assay type: RNA-seq Profiling

Technology type: Next generation sequencing

Investigation: L-fucose degradation in Sulfolobus solfataricus P2

Study: Comparison of S. solfataricus grown on l-fucose...

Organisms: Sulfolobus solfataricus

SOPs: SulfoSYS_BIOTECH: RNA isolation and sequencing

Data files: Transcriptomics L-fuc vs D-glc

Snapshots: No snapshots

Created: 17th Dec 2014 at 13:08, Last updated: 8th Nov 2017 at 14:21

intracellular metabolome analysis: l-fucose vs d-glucose

SulfoSys - Biotec

(Show All)

No description specified

Submitter: Theresa Kouril

Assay type: Metabolite Profiling

Technology type: Gas Chromatography Mass Spectrometry

Investigation: L-fucose degradation in Sulfolobus solfataricus P2

Study: Comparison of S. solfataricus grown on l-fucose...

Organisms: Sulfolobus solfataricus

SOPs: No SOPs

Data files: Metabolomics L-fuc vs D-glc

Snapshots: No snapshots

Created: 5th Feb 2015 at 11:51, Last updated: 8th Nov 2017 at 14:21

Proteome analysis: d-fuc / l-glu

SulfoSys - Biotec

(Show All)

No description specified

Submitter: Theresa Kouril

Assay type: Proteomics

Technology type: Tandem Mass Spectrometry

Investigation: L-fucose degradation in Sulfolobus solfataricus P2

Study: Comparison of S. solfataricus grown on l-fucose...

Organisms: Sulfolobus solfataricus

SOPs: SULFOSYSbiotech_Proteome analysis

Data files: Proteomics L-fuc vs D-glc

Snapshots: No snapshots

Created: 5th Feb 2015 at 12:42, Last updated: 8th Nov 2017 at 14:21

Cell free extract activity measurements: L-fuc/d-glc

SulfoSys - Biotec

(Show All)

No description specified

Submitter: Jacqueline Wolf

Assay type: Experimental Assay Type

Technology type: Enzymatic Activity Measurements

Investigation: L-fucose degradation in Sulfolobus solfataricus P2

Study: Comparison of S. solfataricus grown on l-fucose...

Organisms: Sulfolobus solfataricus

SOPs: No SOPs

Data files: Activity assay L-fuc vs D-glc

Snapshots: No snapshots

Created: 3rd Dec 2015 at 07:24, Last updated: 8th Nov 2017 at 14:21

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