SARCHI: Mechanistic modelling of health and epidemiology
The Snoep Lab’s core research efforts are in Computational Systems Biology; a combined experimental, modeling and theoretical approach to quantitatively understand the functional behavior of Biological Systems resulting from the characteristics of their components. Our main focus is on metabolism, of human pathogens such as Plasmodium falciparum, Mycobacterium tuberculosis, but also of breast cancer cell lines, and on modelling disease states such as glucose homeostatis in type 2 diabetes, and malaria patients at a whole body level. Ultimately the whole body disease state models will be linked to epidemiology for transmitable diseases such as malaria.
Prof. Snoep holds a South African Research Chair (SARChI) that is linked to the South African Centre of Excellence in Epidemiological Modelling and Analysis (SACEMA) with the NRF and DSI as funders.
Web page: http://www.sun.ac.za/english/faculty/science/biochemistry/research/snoep-group
Funding details:South African Research Chair in: "Mechanistic modelling of health and epidemiology" hosted by Stellenbosch University, funded by the DST and administered by NRF.
Related items
Projects: SulfoSys, SulfoSys - Biotec, Glucose metabolism in cancer cell lines
Institutions: University Duisburg-Essen, Stellenbosch University
Projects: SulfoSys, SulfoSys - Biotec, HOTSOLUTE, Computational pathway design for biotechnological applications, SCyCode The Autotrophy-Heterotrophy Switch in Cyanobacteria: Coherent Decision-Making at Multiple Regulatory Layers
Institutions: University Duisburg-Essen, Universitity Duisburg-Essen
https://orcid.org/0000-0002-9905-541X
Head of the group of Molecular Enzyme Technology and Biochemistry (Faculty of Chemistry) at the University of Duisburg-Essen. My research interest is on archaeal physiology with a special focuss on the central carbohydrate metabolism of (hyper)thermophilic Archaea and its regulation. The aim is to gain a systems level understanding by the combination of modern highthrouput analyses with classical biochemistry and molecular biology. Archaea possess many novel enzymes and pathways and our aim is ...
Projects: PSYSMO, MOSES, SysMO DB, SysMO-LAB, SulfoSys, SulfoSys - Biotec, Whole body modelling of glucose metabolism in malaria patients, FAIRDOM, Molecular Systems Biology, COMBINE Multicellular Modelling, HOTSOLUTE, Steroid biosynthesis, Yeast glycolytic oscillations, Computational pathway design for biotechnological applications, SCyCode The Autotrophy-Heterotrophy Switch in Cyanobacteria: Coherent Decision-Making at Multiple Regulatory Layers, Project Coordination, WP 3: Drug release kinetics study, Glucose metabolism in cancer cell lines
Institutions: Manchester Centre for Integrative Systems Biology, University of Manchester, University of Stellenbosch, University of Manchester - Department of Computer Science, Stellenbosch University
Projects: SysMO DB, Whole body modelling of glucose metabolism in malaria patients, Manchester Institute for Biotechnology, FAIRDOM, ICYSB 2015 - International Practical Course in Systems Biology, GenoSysFat, DigiSal, FAIRDOM user meeting
Institutions: University of Manchester - Department of Computer Science, Manchester Centre for Integrative Systems Biology, University of Manchester
https://orcid.org/0000-0003-4958-0184
Interested in systems + synthetic biology, biotechnology, mountaineering, swimming, running, and the occasional cup of tea. Once diagnosed as an ENFP.
Up-regulation of glycolytic flux (glucose to lactate conversion), also known as the “Warburg effect”, has been associated with 90% of cancers. We implemented a systems biology approach in order to improve the overall understanding of the “Warburg effect” as well as identifying drug targets within the glycolytic pathway.
Programme: SARCHI: Mechanistic modelling of health and epidemiology
Public web page: Not specified
Programme: SARCHI: Mechanistic modelling of health and epidemiology
Public web page: Not specified
Programme: SARCHI: Mechanistic modelling of health and epidemiology
Public web page: Not specified
Hypoglycaemia and lactic acidosis are key diagnostics for poor chances of survival in malaria patients. In this project we aim to test to what extent the metabolic activity of Plasmodium falciparum contributes to a changed glucose metabolism in malaria patients. The approach is to start with detailed bottom up models for the parasite and then merge these with more coarse grained models at the whole body level.
Programme: SARCHI: Mechanistic modelling of health and epidemiology
Public web page: Not specified
The goal of the project is to establish a new biotechnological platform for the production of hydroxy-amino acids, since the current production of these important building blocks is very expensive. Enzyme engineering, systems biotechnology and metabolic engineering will be used in a synthetic biology approach.
Programme: SARCHI: Mechanistic modelling of health and epidemiology
Public web page: Not specified
The figures 2, 3, 4 and 6 in the main text of the manuscript: "Inhibition of the glucocorticoid-activating enzyme 11β-hydroxysteroid dehydrogenase type 1 drives concurrent 11-oxygenated androgen excess", submitted to FASEB by Lina Schiffer, Imken Oestlund, Jacky Snoep, Lorna C. Gilligan, Angela E. Taylor, Alexandra J. Sinclair, Rishi Singhal, Adrian Freeman, Ramzi Ajjan, Ana Tiganescu, Wiebke Arlt and Karl-Heinz Storbeck, are reproduced in Mathematica notebooks. The notebooks and the corresponding ...
Submitter: Jacky Snoep
Studies: CBX inhibition of HSD11B1 and effect on HSD11B1/AKR1C3 incubations, Computational analysis of combined HSD11B1/AKR1C3 ratios and HSD11B1 inh..., HSD11B1/AKR1C3 ratio experiments, Inhibition of HSD11B1 in adipose tissue
Assays: Data for HSD11B1/AKR1C3 ratio experiment in Fig. 2, Experimental data for HSD11B1/AKR1C3 incubation with CBX inhibition, Inhibition of HSD11B1 in adipose tissue, Model analysis for HSD11B1/AKR1C3 ratio experiment, Model for computational analysis of HSD11B1/AKR1C3 ratio variation and H..., Model simulations of HSD11B1/AKR1C3 incubation and cortisone and 11KA4 i..., Simulation of HSD11B1 inhibition in human adipose tissue
Submitter: Theresa Kouril
Studies: 3-Bromopyruvate (3BrP) titrations, Data analysis and calculation of control coefficients, Iodoacetic acid (IAA) titrations in cancer cell lines
Assays: GAPDH and flux inhibition in MCF7 and MDA231cells, HK, GAPDH and flux inhibition in MDA231 cells, Incubation time for inhibitor treatment, Target verification/ Specificity of 3BrP in glycolysis, Traget verification/ Specificity of IAA in glycolysis
The investigation entails the construction and validation of a detailed mathematical model for glycolysis erythrocytes infected with the malaria parasite Plasmodium falciparum in the blood stage form.
Submitter: Dawie van Niekerk
Studies: Analysis of model for malaria-infected erythrocytes, Intra-erythrocytic malaria parasite volumes, Validation of model for malaria-infected erythrocytes
Assays: Flux vs external glucose, Flux vs parasitaemia, GLC incubation, Inhibition of glycolytic flux, Malaria parasite volume determinations, Metabolic control analysis, Stage specific fluxes, Steady-state
The oxidative Weimberg pathway for the five-step pentose degradation to α ketoglutarate from Caulobacter crescentus is a key route for sustainable bioconversion of lignocellulosic biomass to added-value products and biofuels. Here, we developed a novel iterative approach involving initial rate kinetics, progress curves, and enzyme cascades, with high resolution NMR analysis of intermediate dynamics, and multiple cycles of kinetic modelling analyses to construct and validate a quantitative model ...
Submitter: Jacky Snoep
Studies: Cell free extract, Initial rate kinetics, One pot cascade, Progress curves
Assays: Cell free extract, with Mn and NAD recycling, Cell free extract, with Mn, no NAD recycling, Cell free extract, without added Mn, with NAD recycling, KDXD, KGSADH, One pot cascade 10, One pot cascade 12, One pot cascade 13, One pot cascade 16, Progress curve KDXD, Progress curve KGSADH, Progress curve XAD, Progress curve XDH, Progress curve XLA, Progress curves combined, Steady state cell free extract, with Mn and NAD recycling, XAD, XDH, XLA
Submitter: Dawie van Niekerk
Studies: Allosteric regulation of phosphofructokinase controls the emergence of g..., Heterogeneity of glycolytic oscillatory behaviour in individual yeast cells, Sustained glycolytic oscillations in individual isolated yeast cells
Assays: gustavsson1-4 models, gustavsson5 model
Submitter: Jacky Snoep
Studies: Entrainment of heterogeneous glycolytic oscillations in single cells
Assays: No Assays
Submitter: Jacky Snoep
Studies: Substrate preferences for AKR1C3, and implications of varying AKR1C3:17B...
Assays: No Assays
The investigation entails the construction and validation of a detailed mathematical model for glycolysis of the malaria parasite Plasmodium falciparum in the blood stage trophozoite form.
Submitter: Dawie van Niekerk
Studies: Model analysis, Model construction, Model validation
Assays: ALD, ATPASE, Culturing and synchronisation of P. falciparum, ENO, G3PDH, GAPDH, GLC incubation, GLCtr, GLYtr, HK, Inhibition of glucose transport, Inhibition of lactate flux, LACtr, LDH, PFK, PGI, PGK, PGM, PK, PYRtr, Steady state, Supply-demand analysis, TPI, Trophozoite Isolation and Lysate Preparation
HSD11B1 was inhibited in human subcutaneous and omental adipose tissue, and the effect on oxygenated adrogen metabolism was studied.
Surface plots showing the computational analysis of combined HSD11B1/AKR1C3 ratios and HSD11B1 inhibition.
Submitter: Jacky Snoep
Investigation: Effect of 11BHSD inhibition on 11-ketotestoster...
Assays: Model for computational analysis of HSD11B1/AKR1C3 ratio variation and H...
HSD11B1 was inhibited by CBX and the effect of the inhibition on Cortisone and 11KA4 conversion by HSD11B1/AKR1C3 incubations were measured.
HSD11B1 and AKR1C3 transfected cells were mixed at difefrent ratios and effects on cortisone conversion (Fig. 2B) and 11KT production (Fig. 2C) were measured and simulated.
Submitter: Dawie van Niekerk
Investigation: Glucose metabolism in Plasmodium falciparum inf...
Submitter: Theresa Kouril
Investigation: Metabolic control analysis of glyceraldehyde 3-...
Assays: No Assays
This study includes the experimental data for model validation and the model predictions of that data set.
Submitter: Dawie van Niekerk
Investigation: Glucose metabolism in Plasmodium falciparum inf...
Assays: Inhibition of glycolytic flux, Metabolic control analysis
This study includes the experimental data for model validation and the model predictions of that data set.
Submitter: Dawie van Niekerk
Investigation: Glucose metabolism in Plasmodium falciparum inf...
Assays: Flux vs external glucose, Flux vs parasitaemia, GLC incubation, Stage specific fluxes, Steady-state
One pot cascade - pathway analysis for the purified Caulinobacter crescentus Weimberg pathway enzymes. Effect of co-factor recycling, removal of XLA, and optimisation on Xylose to aKG is studied.
https://jjj.bio.vu.nl/models/experiments/shen2020_fig3a/simulate https://jjj.bio.vu.nl/models/experiments/shen2020_fig3b/simulate https://jjj.bio.vu.nl/models/experiments/shen2020_fig3c/simulate https://jjj.bio.vu.nl/models/experiments/shen2020_fig3d/simulate
Submitter: Jacky Snoep
Investigation: Caulobacter crescentus Weimberg pathway
Assays: One pot cascade 10, One pot cascade 12, One pot cascade 13, One pot cascade 16
Initial rate kinetics for the purified Caulinobacter crescentus Weimberg pathway enzymes, including substrate dependence, and product inhibition.
Submitter: Jacky Snoep
Investigation: Caulobacter crescentus Weimberg pathway
Progress curves for the purified Caulinobacter crescentus Weimberg pathway enzymes. Each reaction is followed up to completion and then the next enzyme in the pathway is added, i.e. XDH-XLA-XAD-KDXD and finally KGSADH
Submitter: Jacky Snoep
Investigation: Caulobacter crescentus Weimberg pathway
Assays: Progress curve KDXD, Progress curve KGSADH, Progress curve XAD, Progress curve XDH, Progress curve XLA, Progress curves combined
Cell free extract - pathway analysis for Caulinobacter crescentus Weimberg pathway enzymes. Effect of co-factor recycling, and Mn2+ on Xylose to aKG conversion is studied.
Submitter: Dawie van Niekerk
Investigation: From steady-state to synchronized yeast glycoly...
Assays: No Assays
Submitter: Dawie van Niekerk
Investigation: From steady-state to synchronized yeast glycoly...
Assays: No Assays
Submitter: Dawie van Niekerk
Investigation: Glycolytic oscillations in individual isolated ...
Assays: gustavsson1-4 models
Submitter: Dawie van Niekerk
Investigation: Glycolytic oscillations in individual isolated ...
Assays: No Assays
Submitter: Dawie van Niekerk
Investigation: Glycolytic oscillations in individual isolated ...
Assays: gustavsson5 model
Submitter: Dawie van Niekerk
Investigation: Phase shift responses in isolated yeast glycoly...
Assays: No Assays
Kinetic characterisation en mathematical modelling of HK.
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Kinetic characterisation en mathematical modelling of PGI.
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Kinetic characterisation en mathematical modelling of PFK.
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Kinetic characterisation en mathematical modelling of ALD.
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Kinetic characterisation en mathematical modelling of TPI.
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Kinetic characterisation en mathematical modelling of G3PDH.
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Kinetic characterisation en mathematical modelling of GAPDH.
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Kinetic characterisation en mathematical modelling of PGK.
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Kinetic characterisation en mathematical modelling of PGM.
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Kinetic characterisation en mathematical modelling of ENO.
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Kinetic characterisation en mathematical modelling of PK.
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Kinetic characterisation and mathematical modelling of LDH.
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
SED-ML simulation: https://jjj.bio.vu.nl/models/experiments/penkler2aa_experiment-user/simulate
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model validation
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Submitter: Dawie van Niekerk
Biological problem addressed: Model Analysis Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model validation
Submitter: Dawie van Niekerk
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Excel spreadsheet with 72h time points for metabolism of cortisone or 11KA4 in human subcutaneous or omental adipose tissue in presence or absence of AZD4017 inhibitor.
Creator: Jacky Snoep
Submitter: Jacky Snoep
DownloadHSD11B1 was inhibited by CBX and the effect on the conversion of cortisone and 11KA4 in HSD11B1/AKR1C3 incubations was followed.
Creator: Jacky Snoep
Submitter: Jacky Snoep
Metabolite profiles after 24h incubation with different ratios of HSD11B1 and AKR1C3 transfected HEK293 cells.
Creator: Jacky Snoep
Submitter: Jacky Snoep
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Volumes in fL, time in hours.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Creator: Theresa Kouril
Submitter: Theresa Kouril
Creator: Theresa Kouril
Submitter: Theresa Kouril
Creator: Theresa Kouril
Submitter: Theresa Kouril
Creator: Theresa Kouril
Submitter: Theresa Kouril
Creator: Theresa Kouril
Submitter: Theresa Kouril
Creator: Theresa Kouril
Submitter: Theresa Kouril
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Data files for the conversion of XYL to KG, via the sequential addition of the Caulobacter crescentus Weimberg pathway enzymes, XDH, XLA, XAD, KDXD, KGSADH.
Creators: Jacky Snoep, Lu Shen
Submitter: Jacky Snoep
Initial rate kinetics for the α-ketoglutarate semialdehyde dehydrogenase of Caulobacter crescentus, α-ketoglutarate semialdehyde and NAD saturation, and α-ketoglutarate, NADH and 2-keto-3-deoxy-D-xylonate inhibition.
Creators: Jacky Snoep, Lu Shen
Submitter: Jacky Snoep
Initial rate kinetics for the 2-keto-3-deoxy-D-xylonate dehydrates of Caulobacter crescentus, 2-keto-3-deoxy-D-xylonate saturation and inhibitor titrations.
Creators: Jacky Snoep, Lu Shen
Submitter: Jacky Snoep
Initial rate kinetics for the xylonate dehydratase of Caulobacter crescentus, xylonate saturation.
Creators: Jacky Snoep, Lu Shen
Submitter: Jacky Snoep
HSD11B1 inhibition by AZD4017 and the effect on cortisone and 11KA4 metabolism was simulated in Mathematica. Figure 6 of the manuscript is reproduced in the notebook.
Creator: Jacky Snoep
Submitter: Jacky Snoep
Model type: Ordinary differential equations (ODE)
Model format: Mathematica
Environment: Mathematica
Mathematica notebook for simulation of combined effect of HSD11B1/AKR1C3 ratio variation and HSD11B1 inhibition, surface plots are generated shown in Fig. 4 of the manuscript.
Creator: Jacky Snoep
Submitter: Jacky Snoep
Model type: Ordinary differential equations (ODE)
Model format: Not specified
Environment: Mathematica
HSD11B1 was inhibited by CBX and the effect on cortisone and 11KA4 conversion was simulated. Model simulated in Mathematica, Figure 3 panels are presented.
Creator: Jacky Snoep
Submitter: Jacky Snoep
Model type: Ordinary differential equations (ODE)
Model format: Mathematica
Environment: Mathematica
Mathematica notebook with model simulation of metabolite profiles after 24h incubation with different ratios of HSD11B1 and AKR1C3 transfected HEK293 cells.
Creator: Jacky Snoep
Submitter: Jacky Snoep
Model type: Ordinary differential equations (ODE)
Model format: Not specified
Environment: Mathematica
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Model type: Ordinary differential equations (ODE)
Model format: SBML
Environment: JWS Online
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Model type: Ordinary differential equations (ODE)
Model format: SBML
Environment: JWS Online
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Model type: Ordinary differential equations (ODE)
Model format: SBML
Environment: JWS Online
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Model type: Ordinary differential equations (ODE)
Model format: Mathematica
Environment: Not specified
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Model type: Ordinary differential equations (ODE)
Model format: Mathematica
Environment: Mathematica
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Model type: Ordinary differential equations (ODE)
Model format: Mathematica
Environment: Mathematica
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Model type: Ordinary differential equations (ODE)
Model format: Mathematica
Environment: Mathematica
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Model type: Ordinary differential equations (ODE)
Model format: Mathematica
Environment: Mathematica
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Model type: Ordinary differential equations (ODE)
Model format: Mathematica
Environment: Mathematica
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Model type: Ordinary differential equations (ODE)
Model format: Mathematica
Environment: Mathematica
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Model type: Ordinary differential equations (ODE)
Model format: Mathematica
Environment: Not specified
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Model type: Ordinary differential equations (ODE)
Model format: Mathematica
Environment: Mathematica
Model for the Caulobacter crescentus Weimberg pathway, describing the conversion of Xyl to KG upon sequential adition of purified enzymes. If the Mathematica notebook is downloaded and the data file is downloaded in the same directory, then the notebook can be evaluated, and the figure in the manuscript for the progress curves will be reproduced.
Creator: Jacky Snoep
Submitter: Jacky Snoep
Model type: Algebraic equations
Model format: Mathematica
Environment: Mathematica
Model for the Caulobacter crescentus Weimberg pathway, describing the conversion of Xyl to KG upon sequential adition of purified enzymes. If the Mathematica notebook is downloaded and the data file is downloaded in the same directory, then the notebook can be evaluated, and the figure in the manuscript for the progress curves will be reproduced.
Creator: Jacky Snoep
Submitter: Jacky Snoep
Model type: Algebraic equations
Model format: Mathematica
Environment: Mathematica
Model for the Caulobacter crescentus Weimberg pathway, describing the conversion of Xyl to KG upon sequential adition of purified enzymes. If the Mathematica notebook is downloaded and the data file is downloaded in the same directory, then the notebook can be evaluated, and the figure in the manuscript for the progress curves will be reproduced.
Creator: Jacky Snoep
Submitter: Jacky Snoep
Model type: Algebraic equations
Model format: Mathematica
Environment: Mathematica
Model for the Caulobacter crescentus Weimberg pathway, describing the conversion of Xyl to KG upon sequential adition of purified enzymes. If the Mathematica notebook is downloaded and the data file is downloaded in the same directory, then the notebook can be evaluated, and the figure in the manuscript for the progress curves will be reproduced.
Creator: Jacky Snoep
Submitter: Jacky Snoep
Model type: Algebraic equations
Model format: Mathematica
Environment: Mathematica
Creator: Theresa Kouril
Submitter: Theresa Kouril
Creator: Theresa Kouril
Submitter: Theresa Kouril
Creator: Theresa Kouril
Submitter: Theresa Kouril
Creator: Theresa Kouril
Submitter: Theresa Kouril
SOP for the determination of external metabolites (Glc, Pyr, Gly, Lac) in intact trophozoite incubations, and for the determination of intracellular metabolite concentrations.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for the cultivation conditions of Plasmodium falciparum, including the protocol for synchronisation.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of G3PDH activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of ALD activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of glucose transport activity in intact trophozoites.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of GAPDH activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of ENO activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of HK activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of PFK activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of LDH activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of PGK activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of PK activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of PGM activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of PGI activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of PGM activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of TPI activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
Abstract
Authors: Theresa Kouril, Craig October, Stephanie Hollocks, Christoff Odendaal, David D. van Niekerk, Jacky L. Snoep
Date Published: 1st Sep 2023
Publication Type: Journal
Abstract (Expand)
Authors: Lu Shen, Martha Kohlhaas, Junichi Enoki, Roland Meier, Bernhard Schönenberger, Roland Wohlgemuth, Robert Kourist, Felix Niemeyer, David van Niekerk, Christopher Bräsen, Jochen Niemeyer, Jacky Snoep, Bettina Siebers
Date Published: 1st Dec 2020
Publication Type: Not specified
Abstract (Expand)
Authors: David D. van Niekerk, Anna-Karin Gustavsson, Martin Mojica-Benavides, Caroline B. Adiels, Mattias Goksör, Jacky L. Snoep
Date Published: 31st Jan 2019
Publication Type: Journal
Abstract (Expand)
Authors: Monique Barnard, Jonathan L. Quanson, Elahe Mostaghel, Elzette Pretorius, Jacky L. Snoep, Karl-Heinz Storbeck
Date Published: 1st Oct 2018
Publication Type: Not specified
Abstract (Expand)
Authors: David D. van Niekerk, Gerald P. Penkler, Francois du Toit, Jacky L. Snoep
Date Published: 1st Feb 2016
Publication Type: Not specified
Abstract (Expand)
Authors: J. L. Snoep, K. Green, J. Eicher, D. C. Palm, G. Penkler, F. du Toit, N. Walters, R. Burger, H. V. Westerhoff, D. D. van Niekerk
Date Published: 27th Nov 2015
Publication Type: Not specified
Abstract (Expand)
Authors: Anna-Karin Gustavsson, Caroline B. Adiels, Bernhard Mehlig, Mattias Goksör
Date Published: 1st Aug 2015
Publication Type: Not specified
Abstract (Expand)
Authors: Gerald Penkler, Francois du Toit, Waldo Adams, Marina Rautenbach, Daniel C. Palm, David D. van Niekerk, Jacky L. Snoep
Date Published: 1st Apr 2015
Publication Type: Not specified
Abstract
Authors: Anna-Karin Gustavsson, David D. van Niekerk, Caroline B. Adiels, Bob Kooi, Mattias Goksör, Jacky L. Snoep
Date Published: 1st Jun 2014
Publication Type: Not specified
Abstract
Authors: Anna-Karin Gustavsson, David D. van Niekerk, Caroline B. Adiels, Mattias Goksör, Jacky L. Snoep
Date Published: 3rd Jan 2014
Publication Type: Not specified
Abstract
Authors: Anna-Karin Gustavsson, David D. van Niekerk, Caroline B. Adiels, Franco B. du Preez, Mattias Goksör, Jacky L. Snoep
Date Published: 1st Aug 2012
Publication Type: Not specified
Abstract (Expand)
Authors: F. B. du Preez, D. D. van Niekerk, J. L. Snoep
Date Published: No date defined
Publication Type: Not specified
Abstract (Expand)
Authors: F. B. du Preez, D. D. van Niekerk, B. Kooi, J. M. Rohwer, J. L. Snoep
Date Published: No date defined
Publication Type: Not specified
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
