SEEK ID: https://fairdomhub.org/assays/266
Investigation: Glucose metabolism in Plasmodium falciparum trophozoites
Study: Model validation
Biological problem addressed: Model Analysis Type
Organisms: No organisms
Created: 15th Aug 2014 at 14:58
Last updated: 8th Nov 2017 at 15:21
Hypoglycaemia and lactic acidosis are key diagnostics for poor chances of survival in malaria patients. In this project we aim to test to what extent the metabolic activity of Plasmodium falciparum contributes to a changed glucose metabolism in malaria patients. The approach is to start with detailed bottom up models for the parasite and then merge these with more coarse grained models at the whole body level.
Public web page: Not specified
The investigation entails the construction and validation of a detailed mathematical model for glycolysis of the malaria parasite Plasmodium falciparum in the blood stage trophozoite form.
Assays: ALD, ATPASE, Culturing and synchronisation of P. falciparum, ENO, G3PDH, GAPDH, GLC incubation, GLCtr, GLYtr, HK, Inhibition of glucose transport, Inhibition of lactate flux, LACtr, LDH, PFK, PGI, PGK, PGM, PK, PYRtr, Steady state, Supply-demand analysis, TPI, Trophozoite Isolation and Lysate Preparation
This study includes the experimental data for model validation and the model predictions of that data set.
Person responsible: Dawie van Niekerk
Snapshots: No snapshots
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Glycolytic model for Plasmodium falciparum; open system
Submitter: Dawie van Niekerk
Model type: Ordinary differential equations (ODE)
Model format: SBML
Environment: JWS Online
SOP for the determination of external metabolites (Glc, Pyr, Gly, Lac) in intact trophozoite incubations, and for the determination of intracellular metabolite concentrations.
Investigations: Glucose metabolism in Plasmodium falciparum tro...
Studies: Model validation