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This study includes the experimental data for model validation and the model predictions of that data set.
FAIRDOMHub ID: https://fairdomhub.org/studies/119
Glucose metabolism in Plasmodium falciparum trophozoites
Projects: Whole body modelling of glucose metabolism in malaria patients
Experimentalists: Gerald Penkler, Francois du Toit, Waldo Adams

Views: 1510
Created: 11th Aug 2014 at 09:43
Last updated: 11th Dec 2014 at 10:46
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- Investigations (1)
- Assays (2)
- Data files (1)
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- SOPs (1)
- Publications (1)
Hypoglycaemia and lactic acidosis are key diagnostics for poor chances of survival in malaria patients. In this project we aim to test to what extent the metabolic activity of Plasmodium falciparum contributes to a changed glucose metabolism in malaria patients. The approach is to start with detailed bottom up models for the parasite and then merge these with more coarse grained models at the whole body level.
Programme: SARCHI: Mechanistic modelling of health and epidemiology
Public web page: Not specified
Organisms: Plasmodium falciparum, Homo sapiens
The investigation entails the construction and validation of a detailed mathematical model for glycolysis of the malaria parasite Plasmodium falciparum in the blood stage trophozoite form.
Snapshots: Snapshot 1
Studies: Model analysis, Model construction, Model validation
Assays: ALD, ATPASE, Culturing and synchronisation of P. falciparum, ENO, G3PDH, GAPDH, GLC incubation, GLCtr, GLYtr, HK, Inhibition of glucose transport, Inhibition of lactate flux, LACtr, LDH, PFK, PGI, PGK, PGM, PK, PYRtr, Steady state, Supply-demand analysis, TPI, Trophozoite Isolation and Lysate Preparation
SED-ML simulation: https://jjj.bio.vu.nl/models/experiments/penkler2aa_experiment-user/simulate
Contributor: Dawie Van Niekerk
Biological problem addressed: Model Analysis Type
Snapshots: No snapshots
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model validation
Organisms: No organisms
Models: Kinetic model for incubation (penkler2)
SOPs: Validation experiments
Data files: 5mM GLC runout data.
Contributor: Dawie Van Niekerk
Biological problem addressed: Model Analysis Type
Snapshots: No snapshots
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model validation
Organisms: No organisms
Models: Kinetic model for steady state (penkler1)
SOPs: Validation experiments
Data files: No Data files
Timecourses of GLC (starting at 5 mM) and LAC, PYR, GLY in the closed system.
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
Investigations: Glucose metabolism in Plasmodium falciparum tro...
Studies: Model validation
Assays: GLC incubation
Glycolytic model for Plasmodium falciparum; open system
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
Model type: Ordinary differential equations (ODE)
Model format: SBML
Environment: JWS Online
Organism: Not specified
Investigations: Glucose metabolism in Plasmodium falciparum tro...
Studies: Model analysis, Model validation
Modelling analyses: Inhibition of lactate flux, Steady state, Supply-demand analysis
Glycolytic model for Plasmodium falciparum; closed system
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
Model type: Ordinary differential equations (ODE)
Model format: SBML
Environment: JWS Online
Organism: Not specified
Investigations: Glucose metabolism in Plasmodium falciparum tro...
Studies: Model validation
Modelling analyses: GLC incubation
SOP for the determination of external metabolites (Glc, Pyr, Gly, Lac) in intact trophozoite incubations, and for the determination of intracellular metabolite concentrations.
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
Investigations: Glucose metabolism in Plasmodium falciparum tro...
Studies: Model validation
Assays: GLC incubation, Steady state
Abstract (Expand)
Authors: Dawie Van Niekerk, Jacky Snoep, Penkler Gerald,du Toit Francois,Adams Waldo,Rautenbach Marina,Palm Daniel C.
Date Published: 1st Apr 2015
Journal: FEBS J
DOI: 10.1111/febs.13237
Citation: FEBS J 282(8) : 1481