Investigations
What is an Investigation?Filters
The figures 2, 3, 4 and 6 in the main text of the manuscript: "Inhibition of the glucocorticoid-activating enzyme 11β-hydroxysteroid dehydrogenase type 1 drives concurrent 11-oxygenated androgen excess", submitted to FASEB by Lina Schiffer, Imken Oestlund, Jacky Snoep, Lorna C. Gilligan, Angela E. Taylor, Alexandra J. Sinclair, Rishi Singhal, Adrian Freeman, Ramzi Ajjan, Ana Tiganescu, Wiebke Arlt and Karl-Heinz Storbeck, are reproduced in Mathematica notebooks. The notebooks and the corresponding ...
Submitter: Jacky Snoep
Studies: CBX inhibition of HSD11B1 and effect on HSD11B1/AKR1C3 incubations, Computational analysis of combined HSD11B1/AKR1C3 ratios and HSD11B1 inh..., HSD11B1/AKR1C3 ratio experiments, Inhibition of HSD11B1 in adipose tissue
Assays: Data for HSD11B1/AKR1C3 ratio experiment in Fig. 2, Experimental data for HSD11B1/AKR1C3 incubation with CBX inhibition, Inhibition of HSD11B1 in adipose tissue, Model analysis for HSD11B1/AKR1C3 ratio experiment, Model for computational analysis of HSD11B1/AKR1C3 ratio variation and H..., Model simulations of HSD11B1/AKR1C3 incubation and cortisone and 11KA4 i..., Simulation of HSD11B1 inhibition in human adipose tissue
Submitter: Theresa Kouril
Studies: 3-Bromopyruvate (3BrP) titrations, Data analysis and calculation of control coefficients, Iodoacetic acid (IAA) titrations in cancer cell lines
Assays: GAPDH and flux inhibition in MCF7 and MDA231cells, HK, GAPDH and flux inhibition in MDA231 cells, Incubation time for inhibitor treatment, Target verification/ Specificity of 3BrP in glycolysis, Traget verification/ Specificity of IAA in glycolysis
The investigation entails the construction and validation of a detailed mathematical model for glycolysis erythrocytes infected with the malaria parasite Plasmodium falciparum in the blood stage form.
Submitter: Dawie van Niekerk
Studies: Analysis of model for malaria-infected erythrocytes, Intra-erythrocytic malaria parasite volumes, Validation of model for malaria-infected erythrocytes
Assays: Flux vs external glucose, Flux vs parasitaemia, GLC incubation, Inhibition of glycolytic flux, Malaria parasite volume determinations, Metabolic control analysis, Stage specific fluxes, Steady-state
The oxidative Weimberg pathway for the five-step pentose degradation to α ketoglutarate from Caulobacter crescentus is a key route for sustainable bioconversion of lignocellulosic biomass to added-value products and biofuels. Here, we developed a novel iterative approach involving initial rate kinetics, progress curves, and enzyme cascades, with high resolution NMR analysis of intermediate dynamics, and multiple cycles of kinetic modelling analyses to construct and validate a quantitative model ...
Submitter: Jacky Snoep
Studies: Cell free extract, Initial rate kinetics, One pot cascade, Progress curves
Assays: Cell free extract, with Mn and NAD recycling, Cell free extract, with Mn, no NAD recycling, Cell free extract, without added Mn, with NAD recycling, KDXD, KGSADH, One pot cascade 10, One pot cascade 12, One pot cascade 13, One pot cascade 16, Progress curve KDXD, Progress curve KGSADH, Progress curve XAD, Progress curve XDH, Progress curve XLA, Progress curves combined, Steady state cell free extract, with Mn and NAD recycling, XAD, XDH, XLA
Submitter: Dawie van Niekerk
Studies: Allosteric regulation of phosphofructokinase controls the emergence of g..., Heterogeneity of glycolytic oscillatory behaviour in individual yeast cells, Sustained glycolytic oscillations in individual isolated yeast cells
Assays: gustavsson1-4 models, gustavsson5 model
Submitter: Jacky Snoep
Studies: Entrainment of heterogeneous glycolytic oscillations in single cells
Assays: No Assays
Submitter: Jacky Snoep
Studies: Substrate preferences for AKR1C3, and implications of varying AKR1C3:17B...
Assays: No Assays
The investigation entails the construction and validation of a detailed mathematical model for glycolysis of the malaria parasite Plasmodium falciparum in the blood stage trophozoite form.
Submitter: Dawie van Niekerk
Studies: Model analysis, Model construction, Model validation
Assays: ALD, ATPASE, Culturing and synchronisation of P. falciparum, ENO, G3PDH, GAPDH, GLC incubation, GLCtr, GLYtr, HK, Inhibition of glucose transport, Inhibition of lactate flux, LACtr, LDH, PFK, PGI, PGK, PGM, PK, PYRtr, Steady state, Supply-demand analysis, TPI, Trophozoite Isolation and Lysate Preparation
