HSD11B1/AKR1C3 ratio experiments
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HSD11B1 and AKR1C3 transfected cells were mixed at difefrent ratios and effects on cortisone conversion (Fig. 2B) and 11KT production (Fig. 2C) were measured and simulated.
SEEK ID: https://fairdomhub.org/studies/1250
Effect of 11BHSD inhibition on 11-ketotestosterone biosynthesis
Projects: Steroid biosynthesis
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Created: 16th Feb 2024 at 11:26
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Projects: PSYSMO, MOSES, SysMO DB, SysMO-LAB, SulfoSys, SulfoSys - Biotec, Whole body modelling of glucose metabolism in malaria patients, FAIRDOM, Molecular Systems Biology, COMBINE Multicellular Modelling, HOTSOLUTE, Steroid biosynthesis, Yeast glycolytic oscillations, Computational pathway design for biotechnological applications, SCyCode The Autotrophy-Heterotrophy Switch in Cyanobacteria: Coherent Decision-Making at Multiple Regulatory Layers, Project Coordination, WP 3: Drug release kinetics study, Glucose metabolism in cancer cell lines
Institutions: Manchester Centre for Integrative Systems Biology, University of Manchester, University of Stellenbosch, University of Manchester - Department of Computer Science, Stellenbosch University
The Snoep Lab’s core research efforts are in Computational Systems Biology; a combined experimental, modeling and theoretical approach to quantitatively understand the functional behavior of Biological Systems resulting from the characteristics of their components. Our main focus is on metabolism, of human pathogens such as Plasmodium falciparum, Mycobacterium tuberculosis, but also of breast cancer cell lines, and on modelling disease states such as glucose homeostatis in type 2 diabetes, and ...
Projects: Whole body modelling of glucose metabolism in malaria patients, Steroid biosynthesis, Yeast glycolytic oscillations, Computational pathway design for biotechnological applications, Glucose metabolism in cancer cell lines
Web page: http://www.sun.ac.za/english/faculty/science/biochemistry/research/snoep-group
Programme: SARCHI: Mechanistic modelling of health and epidemiology
Public web page: Not specified
The figures 2, 3, 4 and 6 in the main text of the manuscript: "Inhibition of the glucocorticoid-activating enzyme 11β-hydroxysteroid dehydrogenase type 1 drives concurrent 11-oxygenated androgen excess", submitted to FASEB by Lina Schiffer, Imken Oestlund, Jacky Snoep, Lorna C. Gilligan, Angela E. Taylor, Alexandra J. Sinclair, Rishi Singhal, Adrian Freeman, Ramzi Ajjan, Ana Tiganescu, Wiebke Arlt and Karl-Heinz Storbeck, are reproduced in Mathematica notebooks. The notebooks and the corresponding ...
Submitter: Jacky Snoep
Studies: CBX inhibition of HSD11B1 and effect on HSD11B1/AKR1C3 incubations, Computational analysis of combined HSD11B1/AKR1C3 ratios and HSD11B1 inh..., HSD11B1/AKR1C3 ratio experiments, Inhibition of HSD11B1 in adipose tissue
Assays: Data for HSD11B1/AKR1C3 ratio experiment in Fig. 2, Experimental data for HSD11B1/AKR1C3 incubation with CBX inhibition, Inhibition of HSD11B1 in adipose tissue, Model analysis for HSD11B1/AKR1C3 ratio experiment, Model for computational analysis of HSD11B1/AKR1C3 ratio variation and H..., Model simulations of HSD11B1/AKR1C3 incubation and cortisone and 11KA4 i..., Simulation of HSD11B1 inhibition in human adipose tissue
Excel file with metablite profiles after 24 h incubation with different ratios of 11HSDB1 and AKR1C3.
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Effect of 11BHSD inhibition on 11-ketotestoster...
Model simulations for 24h incubations with different ratios of HSD11B1 andd AKR1C3 transfected HEK293 cells are given in Mathematica notebook format.
Submitter: Jacky Snoep
Biological problem addressed: Model Analysis Type
Investigation: Effect of 11BHSD inhibition on 11-ketotestoster...
Metabolite profiles after 24h incubation with different ratios of HSD11B1 and AKR1C3 transfected HEK293 cells.
Creator: Jacky Snoep
Submitter: Jacky Snoep
Mathematica notebook with model simulation of metabolite profiles after 24h incubation with different ratios of HSD11B1 and AKR1C3 transfected HEK293 cells.
Creator: Jacky Snoep
Submitter: Jacky Snoep
Model type: Ordinary differential equations (ODE)
Model format: Not specified
Environment: Mathematica
