SOPs

What is a SOP?

21 SOPs visible to you, out of a total of 21

SOP_Preparation of cell lysates

Glucose metabolism in cancer cell lines

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: Metabolic control analysis of glyceraldehyde 3-...

Studies: 3-Bromopyruvate (3BrP) titrations, Iodoacetic acid (IAA) titrations in cancer cell...

Assays: GAPDH and flux inhibition in MCF7 and MDA231cells, HK, GAPDH and flux inhibition in MDA231 cells, Incubation time for inhibitor treatment, Target verification/ Specificity of 3BrP in gly...

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Created: 13th Jul 2023 at 12:52

SOP_Inhibitor titration intact cells

Glucose metabolism in cancer cell lines

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: Metabolic control analysis of glyceraldehyde 3-...

Studies: 3-Bromopyruvate (3BrP) titrations, Iodoacetic acid (IAA) titrations in cancer cell...

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Created: 13th Jul 2023 at 12:49

SOP_Quantification of glycolytic flux (glucose, lactate)

Glucose metabolism in cancer cell lines

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: Metabolic control analysis of glyceraldehyde 3-...

Studies: 3-Bromopyruvate (3BrP) titrations, Iodoacetic acid (IAA) titrations in cancer cell...

Assays: GAPDH and flux inhibition in MCF7 and MDA231cells, HK, GAPDH and flux inhibition in MDA231 cells, Incubation time for inhibitor treatment

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Created: 12th Jul 2023 at 13:05

SOP_Cancer cell lines_standard cell culture

Glucose metabolism in cancer cell lines

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: Metabolic control analysis of glyceraldehyde 3-...

Studies: 3-Bromopyruvate (3BrP) titrations, Iodoacetic acid (IAA) titrations in cancer cell...

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Created: 12th Jul 2023 at 13:03

Validation experiments

Whole body modelling of glucose metabolism in malaria patients

SOP for the determination of external metabolites (Glc, Pyr, Gly, Lac) in intact trophozoite incubations, and for the determination of intracellular metabolite concentrations.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model validation

Assays: GLC incubation, Steady state

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Created: 15th Aug 2014 at 13:00, Last updated: 17th Apr 2015 at 10:27

Culturing and synchronisation of P. falciparum

Whole body modelling of glucose metabolism in malaria patients

SOP for the cultivation conditions of Plasmodium falciparum, including the protocol for synchronisation.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 15th Aug 2014 at 13:03, Last updated: 3rd Mar 2015 at 09:46

G3PDH Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of G3PDH activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: G3PDH

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Created: 27th Aug 2014 at 12:44, Last updated: 3rd Mar 2015 at 09:42

ALD Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of ALD activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: ALD

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Created: 15th Aug 2014 at 12:46, Last updated: 3rd Mar 2015 at 09:41

GLCtr Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of glucose transport activity in intact trophozoites.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 15th Aug 2014 at 12:42, Last updated: 3rd Mar 2015 at 09:41

GAPDH Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of GAPDH activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: GAPDH

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Created: 15th Aug 2014 at 12:49, Last updated: 3rd Mar 2015 at 09:40

ENO Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of ENO activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: ENO

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Created: 15th Aug 2014 at 12:55, Last updated: 3rd Mar 2015 at 09:40

HK Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of HK activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: HK

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Created: 15th Aug 2014 at 12:43, Last updated: 3rd Mar 2015 at 09:40

PFK Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of PFK activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: PFK

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Created: 15th Aug 2014 at 12:45, Last updated: 3rd Mar 2015 at 09:39

LDH Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of LDH activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: LDH

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Created: 15th Aug 2014 at 12:57, Last updated: 3rd Mar 2015 at 09:39

PGK Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of PGK activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: PGK

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Created: 15th Aug 2014 at 12:52, Last updated: 3rd Mar 2015 at 09:38

PK Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of PK activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: PK

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Created: 15th Aug 2014 at 12:56, Last updated: 3rd Mar 2015 at 09:38

PGM Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of PGM activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 27th Aug 2014 at 10:27, Last updated: 3rd Mar 2015 at 09:38

PGI Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of PGI activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: PGI

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Created: 15th Aug 2014 at 12:44, Last updated: 3rd Mar 2015 at 09:37

PGM Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of PGM activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: PGM

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Created: 15th Aug 2014 at 12:53, Last updated: 3rd Mar 2015 at 09:37

TPI Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of TPI activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: TPI

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Created: 15th Aug 2014 at 12:47, Last updated: 3rd Mar 2015 at 09:36

Trophozoite Isolation and Lysate Preparation

Whole body modelling of glucose metabolism in malaria patients

SOP for the isolation of intact Plasmodium falciparum trophozoites from infected red blood cells and the preparation of a cell free extract that can be used for kinetic analyses.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 15th Aug 2014 at 13:05, Last updated: 3rd Mar 2015 at 07:38

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