Effect of pH on the proteome

SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".

The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.

Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...

Systems Biology of Clostridium acetobutylicum - a possible answer to dwindling crude oil reserves

Investigation of all steady state pH-values between pH 5.7 and 4.5 (pH 5.5, 5.3, 5.1, 4.9, 4.7).

All spots and identified proteins of Clostridium acetobutylicum growing at steady-state pH 5.7 (acidogenesis) and pH 4.5 (solventogenesis), respectively, using 2D PAGE and Maldi-TOF analysis. The focus were cytosolic proteins with an isoelectric point bewteen 4 and 7 as well as a molecular weight of 180-10 kDa.

Representation of all prepared 2D PAGE analyses of cytosolic proteins of Clostridium acetobutylicum growing continuously at pH 5.5, 5.3, 5.1, 4.9 and 4.7.