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Characterization of flux distribution of S. pyogenes M9 wild type and the arcA- and glnA-knockout mutant in continuous cultures
S. pyogenes wildetype, an arcA- and a glnA-deletion mutants were grown in CDM-LAB cultures at pH 6.5 and 7.5 and at a growth rate of 0.05
SEEK ID: https://fairdomhub.org/assays/158
Experimental Assay
Projects: SysMO-LAB
Study: Arginine and Glutamine metabolism in S. pyogenes
Assay type: Experimental Assay Type
Technology type: HPLC
Organisms: Streptococcus pyogenes : M49 (591) (wild-type / wild-type) (chemostat)
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Created: 3rd Apr 2012 at 11:20
Last updated: 8th Nov 2017 at 15:21
Related items
Projects: SysMO-LAB
Institutions: University of Rostock
Expertise: Molecular microbiology, Microbiology
Comparative Systems Biology: Lactic Acid Bacteria
Programme: SysMO
Public web page: http://www.sysmo.net/index.php?index=57
Basically extending SYSMO-LAB 1st phase into second with addition of fourth species, Lb. plantarum. The main focus is amino acid metabolism. primary metabolisms, like glycolysis is also interest.
Snapshots: No snapshots
Studies: Arginine and Glutamine metabolism in S. pyogenes, Determination of essential amino acids for Streptococcus pyogenes
Assays: Characterization of flux distribution of S. pyogenes M9 wild type and th..., Construction of in vivo-like buffer for S. pyogenes, Determination of essential amino acids for Streptococcus pyogenes
Lactic acid bacteria generally use homolactic fermentation for generation of ATP. Here we studied the role of Arginine and Glutamine metabolism on the general physiology of the lactic acid bacteria Streptococcus pyogenes.
A deletion mutant of glnA (glutamine synthetase) has been constructed in the S. pyogenes M49 591 background. The glnA mutant strain shows decreased growth in low glutamine and excess glutamate conditions and no growth at all in low glutamine and low glutamate conditions.
An arcA
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Person responsible: Antje Sieg
Snapshots: No snapshots
Contributor: Araz Zeyniyev
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Streptococcus pyogenes
Genotypes: wild-type
Phenotypes: wild-type
Comment: Not specified
S. pyogenes was grown in C-limited cultures at pH 6.5 and 7.5 and at a growth rate of 0.05
The glnA mutant strain shows decreased growth in low glutamine and excess glutamate conditions and no growth at all in low glutamine and low glutamate conditions. Preliminary results of glucose-limited chemostat cultures indicate a reversion of the pH dependency of the shift from homolactic to more mixed acid fermentation:
wild type - lactate/formate ratio at pH 6.5 = 11.8, at pH 7.5 = 2.8
glnA mutant -
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Creators: Antje Sieg, Silvio Hering, Martijn Bekker
Contributor: Antje Sieg
Relationship type: Not specified
Creator: Martijn Bekker
Contributor: The JERM Harvester
Investigations: Amino acid metabolism of four LAB species: Stre..., Investigation of glycolysis and pyruvate branch...
Studies: Arginine and Glutamine metabolism in S. pyogenes, Comparative modeling and phosphate dependence f...
Assays: Characterization of flux distribution of S. pyo..., Glucose pulsed L. lactis
This HPLC method uses a isocratic method and a RI detector to identify and quantify almost all excreted catabolic metabolites.
Creator: Martijn Bekker
Contributor: Martijn Bekker
Investigations: Amino acid metabolism of four LAB species: Stre..., Investigation of glycolysis and pyruvate branch...
Studies: Arginine and Glutamine metabolism in S. pyogenes, Comparative modeling and phosphate dependence f...
Assays: Characterization of flux distribution of S. pyo..., Glucose pulsed S. pyogenes