M49 (591)
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wild-type
wild-type
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Created: 28th Apr 2010 at 16:21
Last updated: 19th Oct 2012 at 15:57

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Projects: SysMO-LAB
Institutions: University of Rostock
Expertise: Microbiology, Genetics, Molecular Biology, Transcriptomics, Streptococcus pyogenes, Lactococcus, Molecular microbiology, Genetic regulation
Tools: Microbiology, Genetics, Molecular Biology, Genetic modification, Transcriptomics, Microarray analysis, Molecular biology techniques (RNA/DNA/Protein)
Measurements on Km, Vmax and allosteric activation or inhibition of the heterologously expressed (E. coli) and purifiied main L-lactate dehydrogenase
Submitter: Martijn Bekker
Assay type: Enzymatic Assay
Technology type: Enzymatic Activity Measurements
Investigation: Investigation of glycolysis and pyruvate branch...
Organisms: Lactobacillus plantarum : WCFS1 (wild-type / wild-type), Enterococcus faecalis : V583 (wild-type / wild-type), Streptococcus pyogenes : M49 (591) (wild-type / wild-type)
SOPs: Measurement of LDH activity
Data files: The effects of fructose-1,6-bisphosphate, phosp...
Snapshots: No snapshots
L. lactis, E. faecalis and S. pyogenes are referred to as LAB because of the fact that in the presence of glucose lactate is produced as the main fermentation product . This metabolic pathway is relatively inefficient, since only 2 ATP are generated from one glucose molecule. All three LAB possess the genetic make up for mixed acid fermentation, a more effective way of fermentation generating 3 ATP per molecule of glucose. All three genomes reveal (at least) two genes encoding a lactate dehydrogenase ...
Submitter: Martijn Bekker
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Investigation of glycolysis and pyruvate branch...
Organisms: Lactococcus lactis, Streptococcus pyogenes : M49 (591) (wild-type / wild-type), Enterococcus faecalis : V583 (wild-type / wild-type)
SOPs: No SOPs
Data files: Maximal specific growth rates of the three lact...
Snapshots: No snapshots
L. lactis, S. pyogenes and E. faecalis were grown in C-limited chemostat cultures at various pH's and dilution rates. General flux distribution, yields and other physiological factors were studied.
Submitter: Martijn Bekker
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Investigation of glycolysis and pyruvate branch...
Organisms: Lactococcus lactis, Streptococcus pyogenes : M49 (591) (wild-type / wild-type), Enterococcus faecalis : V583 (wild-type / wild-type)
SOPs: No SOPs
Data files: Characterization of flux distribution and gener..., Characterization of flux distribution and gener..., Characterization of flux distribution and gener...
Snapshots: No snapshots
Determination of essential amino acids for Streptococcus pyogenes
Submitter: Araz Zeyniyev
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Amino acid metabolism of four LAB species: Stre...
Organisms: Streptococcus pyogenes : M49 (591) (wild-type / wild-type)
SOPs: No SOPs
Data files: amino acid auxotrophy for streptococcus pyogenes
Snapshots: No snapshots
S. pyogenes wildetype, an arcA- and a glnA-deletion mutants were grown in CDM-LAB cultures at pH 6.5 and 7.5 and at a growth rate of 0.05
Submitter: Antje Sieg
Assay type: Experimental Assay Type
Technology type: HPLC
Investigation: Amino acid metabolism of four LAB species: Stre...
Organisms: Streptococcus pyogenes : M49 (591) (wild-type / wild-type)
SOPs: Analysis of organic acid by HPLC, SOP 2.1 Method for synthesis of LAB-medium used...
Data files: Characterization of chemostats from S. pyogenes...
Snapshots: No snapshots
In order to construct an in vivo-like buffer for S. pyogenes, the intracellular concentrations of Fe, K, Mg, Mn Na, P and S elements were determined via ICP-AES (inductively coupled plasma atomic emissionspectroscopy) method at the Institute of Land Use, University of Rostock. The samples for the analysis were obtained from a steady state culture grown on CDM-LAB with glucose.
Submitter: Araz Zeyniyev
Assay type: Metabolite Profiling
Technology type: Inductively Coupled Plasma Mass Spectrometry
Investigation: Amino acid metabolism of four LAB species: Stre...
Organisms: Streptococcus pyogenes : M49 (591) (wild-type / wild-type)
SOPs: No SOPs
Data files: No Data files
Snapshots: No snapshots
Metabolic network of S. pyogenes including primary metabolism, polysaccharide metabolism, purine and pyrimidine biosoynthesis, teichoic acid biosynthesis, fatty acid and phospholipid bioynthesis, amino acid metabolism, vitamins and cofactors
Submitter: Jennifer Levering
Biological problem addressed: Metabolic Network
Investigation: Investigation of glycolysis and pyruvate branch...
Organisms: Streptococcus pyogenes : M49 (591) (wild-type / wild-type)
Models: Genome-scale model of Streptococcus pyogenes
SOPs: No SOPs
Data files: No Data files
Snapshots: No snapshots