Arginine and Glutamine metabolism in S. pyogenes

Lactic acid bacteria generally use homolactic fermentation for generation of ATP. Here we studied the role of Arginine and Glutamine metabolism on the general physiology of the lactic acid bacteria Streptococcus pyogenes. A deletion mutant of glnA (glutamine synthetase) has been constructed in the S. pyogenes M49 591 background. The glnA mutant strain shows decreased growth in low glutamine and excess glutamate conditions and no growth at all in low glutamine and low glutamate conditions. An arcA deletion mutant has been constructed in the S. pyogenes M49 591 background. In CDM the mutant shows a significantly reduced ammonia production and a significant reduction in growth yield under low glutamine conditions compared to the wild type. The mutant also shows a significantly decrease survival in acidic environments in the presence of arginine in comparison to the wild type.

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Created: 3rd Apr 2012 at 09:13

Last updated: 12th May 2016 at 15:55

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