Determination of essential amino acids for Streptococcus pyogenes M49
SEEK ID: https://fairdomhub.org/studies/70
Projects: SysMO-LAB
Study position:
Experimentalists: Araz Zeyniyev
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Created: 19th May 2011 at 13:32
Last updated: 12th May 2016 at 15:55
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Projects: SysMO-LAB
Institutions: University of Rostock
Expertise: Microbiology, Genetics, Molecular Biology, Transcriptomics, Streptococcus pyogenes, Lactococcus, Molecular microbiology, Genetic regulation
Tools: Microbiology, Genetics, Molecular Biology, Genetic modification, Transcriptomics, Microarray analysis, Molecular biology techniques (RNA/DNA/Protein)
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep
Web page: http://sysmo.net/
Comparative Systems Biology: Lactic Acid Bacteria
Programme: SysMO
Public web page: http://www.sysmo.net/index.php?index=57
Basically extending SYSMO-LAB 1st phase into second with addition of fourth species, Lb. plantarum. The main focus is amino acid metabolism. primary metabolisms, like glycolysis is also interest.
Submitter: Araz Zeyniyev
Studies: Arginine and Glutamine metabolism in S. pyogenes, Determination of essential amino acids for Streptococcus pyogenes
Assays: Characterization of flux distribution of S. pyogenes M9 wild type and th..., Construction of in vivo-like buffer for S. pyogenes, Determination of essential amino acids for Streptococcus pyogenes
Snapshots: No snapshots
Determination of essential amino acids for Streptococcus pyogenes
Submitter: Araz Zeyniyev
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Amino acid metabolism of four LAB species: Stre...
Organisms: Streptococcus pyogenes : M49 (591) (wild-type / wild-type)
SOPs: No SOPs
Data files: amino acid auxotrophy for streptococcus pyogenes
Snapshots: No snapshots
In order to construct an in vivo-like buffer for S. pyogenes, the intracellular concentrations of Fe, K, Mg, Mn Na, P and S elements were determined via ICP-AES (inductively coupled plasma atomic emissionspectroscopy) method at the Institute of Land Use, University of Rostock. The samples for the analysis were obtained from a steady state culture grown on CDM-LAB with glucose.
Submitter: Araz Zeyniyev
Assay type: Metabolite Profiling
Technology type: Inductively Coupled Plasma Mass Spectrometry
Investigation: Amino acid metabolism of four LAB species: Stre...
Organisms: Streptococcus pyogenes : M49 (591) (wild-type / wild-type)
SOPs: No SOPs
Data files: No Data files
Snapshots: No snapshots
amino acid auxotrophy for streptococcus pyogenes in CDM-IMIKRO (not CDM-LAB) as function of final growth yield (OD600 at stationary phase) in 96well plates (200 ul culture)
Creator: Araz Zeyniyev
Submitter: Araz Zeyniyev