Web page: http://www.uni-muenchen.de/index.html
Tools: reporter gene analyses, plasmon resonance spectroscopy, spectroscopy, site-directed and random mutagenesis, molecular biological techniques (RNA/DNA techniques), Membrane protein biochemistry, Chromatography, Fermentation, Biochemistry and protein analysis, Genetics
I am a PhD student of the microbiology department at the Ludwig-Maximilians Universität München. I work at the chair of Prof. Kirsten Jung. The topic of our workpackage deals with "K+ homeostasis in Escherichia coli". In special I'm working on the sensor kinase KdpD that controls together with the response regulator KdpE the expression of the high-affinity K+ uptake system KdpFABC. The yet not fully understood molecular mechanism of stimulus perception and signal transduction is of particular
Tools: qPCR, mutant strain generation, Northern analyses), Fluorecence based reporter gene analyses/single cell analyses, Proteomics (2D-PAGE), enzymatic analyses, Protein chemical methods (protein overproduction, quantitative Western analyses), purification, molecular biological techniques (RNA/DNA techniques
I am research assistant in the microbiology department at the Ludwig-Maximilians Universität in Munich (München), working at the chair of Prof. Kirsten Jung. In our SysMO consortium we generate biological data and work in close cooperation with the workgroup of Dr. Andreas Kremling of the Max-Planck-Institut für Dynamik komplexer technischer Systeme in Magdeburg who performs mathematical modeling. The topic of our workpackage deals with "K+ homeostasis in Escherichia coli", wherby the K+ transporters,
Tools: transcriptional analysis (Northern blots, quantitative RT-PCR); quantitative Western blot analysis; protein purification; reconstitution of membrane-integrated proteins; Biochemistry of sub..., surface plasmon resonance spectroscopy; site-directed spin labeling and EPR analysis;
I have the chair for Microbiology at the Ludwig-Maximilians-Universität München since 2004. My lab has a long-standing interest in stress response and transmembrane signal transduction in E. coli and V. harveyi. Our work is focused on the molecular mechanisms of membrane-integrated receptors, and the systems biological analysis of regulatory circuits.