Controlled Carbon Loss: Threshold-Dependent Overflow Metabolism in Synechocystis
OverviewThis project provides datasets supporting the analysis of carbon partitioning and metabolite excretion in different Synechocystis sp. PCC 6803 strains. It includes intracellular and extracellular metabolite measurements, glycogen quantification, and sampling metadata, enabling investigation of carbon overflow and glycogen metabolism under different nitrogen conditions.
Programme: Independent Projects
SEEK ID: https://fairdomhub.org/projects/510
Public web page: Not specified
Organisms: Synechocystis sp. pcc 6803
FAIRDOM PALs: No PALs for this Project
Project created: 13th Oct 2025
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Projects: Glycogen Metabolism in bacteria, Diverse Metabolic Control of Phosphoglucomutases by Bisphosphorylated Sugars in Heterotrophic Bacteria, Controlled Carbon Loss: Threshold-Dependent Overflow Metabolism in Synechocystis
Institutions: University of Tuebingen
https://orcid.org/0009-0009-1115-5175
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This file contains the processed LC–MS/MS results for intracellular metabolite quantification using the isotope-ratio approach. It provides the C12/C13 ratios for all metabolites in each sample, calculated relative to the 13C-labelled internal standard. The dataset also includes the retention times and the measurement quality scores for each metabolite, enabling assessment of peak identification and data reliability. The raw LC–MS/MS data used to generate these results are available from the ...
Creator: Janette Alford
Submitter: Janette Alford
DownloadThis file contains the data generated for extracellular metabolite quantification using HPLC–MS. It includes the extracted ion chromatograms (EICs) and total ion chromatograms (TICs) for all measured metabolites within the samples and within the calibration standards, as well as the peak heights obtained for both the calibration standards and the extracellular samples. These data support the calculation of metabolite concentrations based on calibration curves.
Creator: Janette Alford
Submitter: Janette Alford
This file contains metadata associated with the metabolomics sampling procedure. It includes (i) optical density (OD) measurements of each culture at the time of sampling for intracellular and extracellular metabolite quantification, and (ii) the sample label table used for intracellular metabolite analysis. The table provides, for each sample, the sample label/number, collected sample volume, quenching volume, and the mapping to the corresponding biological sample.
Creator: Janette Alford
Submitter: Janette Alford
This file contains the data generated for glycogen quantification. It includes the raw absorbance measurements at 540 nm for each sample, the corresponding culture optical densities (OD) at the time of sampling, and the calibration curve data (absorbance values and associated glucose standards) used to calculate glucose concentrations from the measured absorbance values.
Creator: Janette Alford
Submitter: Janette Alford
