Tools: Protein chemical methods (protein overproduction, purification, enzymatic analyses, quantitative Western analyses), molecular biological techniques (RNA/DNA techniques, qPCR, Northern analyses), Proteomics (2D-PAGE), mutant strain generation, Fluorecence based reporter gene analyses/single cell analyses
I am research assistant in the microbiology department at the Ludwig-Maximilians Universität in Munich (München), working at the chair of Prof. Kirsten Jung. In our SysMO consortium we generate biological data and work in close cooperation with the workgroup of Dr. Andreas Kremling of the Max-Planck-Institut für Dynamik komplexer technischer Systeme in Magdeburg who performs mathematical modeling. The topic of our workpackage deals with "K+ homeostasis in Escherichia coli", wherby the K+ transporters, ...
Expertise: Microbiology, Transcriptomics, regulation of gene expression, bacterial gene regulation, Molecular microbiology, Microarray experiments with prokaryotes, Protein-DNA-interaction, Streptomyces, genetic engineering
Tools: Microbiology, Genetic modification, Transcriptomics, PCR, Microarray analysis, Chip-chip, Northern analyses), Bioconductor Packages in R, Molecular biology techniques (RNA/DNA/Protein), Mutant and Strain Construction, site-directed and random mutagenesis, reporter gene analyses, microbiology techniques, analysis of functional genomics data, transcription analysis