Tools: transcriptional analysis (Northern blots, quantitative RT-PCR); quantitative Western blot analysis; protein purification; reconstitution of membrane-integrated proteins; Biochemistry of sub..., surface plasmon resonance spectroscopy; site-directed spin labeling and EPR analysis;
I have the chair for Microbiology at the Ludwig-Maximilians-Universität München since 2004. My lab has a long-standing interest in stress response and transmembrane signal transduction in E. coli and V. harveyi. Our work is focused on the molecular mechanisms of membrane-integrated receptors, and the systems biological analysis of regulatory circuits.
Expertise: Systems Biology, regulation of gene expression, Signal transduction in Gram-negative bacteria; Synthetic Microbiology; Single cell gene expression; Regulatory networks; biochemistry; histidine ki..., gene regulation, stress responses, bistability, sporulation, phenotypic heterogeneity, translational control of gene expression, Bacillus subtilis, Molecular Biology, Genetics, Microbiology
Tools: Time-lapse fluorescence microscopy Flow cytometry, time lapse microscopy, Molecular biology techniques (RNA/DNA), Fluorecence based reporter gene analyses/single cell analyses, PCR, Model organisms, Single Cell analysis, Genetic modification, Genetic analysis, Molecular Biology, Genetics, Microbiology