Institutions: Rostock University Medical Centrehttps://orcid.org/0000-0001-6287-4916
I am an emeritus professor in Biochemistry at the University of Amsterdam (retired 2010).
My research focussed on the human chromatin in its natural environment, i.e. the nucleus of cultured living human cells.
Aspects, such as the dynamic folding of the chromatin fiber inside the nucleus and local chemical modification of histones and DNA at genetic loci, are the physical and chemical basis for epigenetic regulation of gene expression. In my group we worked parallel on human
Expertise: Bacillus subtilis, functional protein expression, bacterial metabolism, carbon metabolism, Bacterial Cell Biology, Protein-DNA-interaction, Microbiology/ Protein chemistry/ Molecular Biology, Cell physiology, regulation of gene expression, quantative biology
Tools: Microbiology, Biochemistry and protein analysis, Cell biology, Model organisms, Chromatography, Molecular biology techniques (RNA/DNA/Protein), DNA, surface plasmon resonance spectroscopy, reporter gene analysis
Tools: Molecular Biology, Cell biology, Computational and theoretical biology, Metabolomics, Model organisms, SBML, ODE, Partial differential equations, Algebraic equations, Linear equations, Copasi, JWS Online, Matlab, Mathematica, SQL, Material balance based modeling
I'm a modeller, specialized in kinetic modeling of biochemical networks. My focus in the SysMO-LAB consortium is on creating models of Lactococcus lactis glycolysis and couple this to other related lactic acid bacteria like Streptococcus pyogenes and Enterococcus faecalis. Besides kinetic modeling, I'm also interested in combining various modeling techniques (genome-scale modeling, qualitative modeling).
Tools: Microbiology, Molecular Biology, Biochemistry and protein analysis, Cell biology, Genetic analysis, Genomics, Transcriptomics, Proteomics, Model organisms, Proteomics (2D-PAGE), gene regulation, DNA technology RNA technology Protein analysis Fermentation Mutagenesis, molecular biological techniques (RNA/DNA techniques), protein interaction studies
Optimisation of Bacillus subtilis for the secretion of heterologous proteins Therapeutic proteins (including those required for experimental purposes and clinical trials) are major products of biomanufacturing processes and considerable time and expense are expended to maximise the yield and quality of proteins produced in heterologous hosts. The production host of choice is the Gram-negative bacterium Escherichia coli for which many strains and expression systems have been developed. However,