Institutions: Rostock University Medical Centrehttps://orcid.org/0000-0001-6287-4916
I am an emeritus professor in Biochemistry at the University of Amsterdam (retired 2010).
My research focussed on the human chromatin in its natural environment, i.e. the nucleus of cultured living human cells.
Aspects, such as the dynamic folding of the chromatin fiber inside the nucleus and local chemical modification of histones and DNA at genetic loci, are the physical and chemical basis for epigenetic regulation of gene expression. In my group we worked parallel on human
Expertise: regulation of gene expression, quantative biology, Cell physiology, Protein-DNA-interaction, Microbiology/ Protein chemistry/ Molecular Biology, Bacterial Cell Biology, carbon metabolism, bacterial metabolism, functional protein expression, Bacillus subtilis
Tools: reporter gene analysis, surface plasmon resonance spectroscopy, DNA, Molecular biology techniques (RNA/DNA/Protein), Chromatography, Model organisms, Cell biology, Biochemistry and protein analysis, Microbiology
Tools: SQL, Material balance based modeling, Mathematica, Matlab, Copasi, JWS Online, Algebraic equations, Linear equations, Partial differential equations, ODE, SBML, Model organisms, Metabolomics, Computational and theoretical biology, Cell biology, Molecular Biology
I'm a modeller, specialized in kinetic modeling of biochemical networks. My focus in the SysMO-LAB consortium is on creating models of Lactococcus lactis glycolysis and couple this to other related lactic acid bacteria like Streptococcus pyogenes and Enterococcus faecalis. Besides kinetic modeling, I'm also interested in combining various modeling techniques (genome-scale modeling, qualitative modeling).
Tools: molecular biological techniques (RNA/DNA techniques), protein interaction studies, DNA technology RNA technology Protein analysis Fermentation Mutagenesis, gene regulation, Proteomics (2D-PAGE), Model organisms, Proteomics, Transcriptomics, Genomics, Genetic analysis, Cell biology, Biochemistry and protein analysis, Molecular Biology, Microbiology
Optimisation of Bacillus subtilis for the secretion of heterologous proteins Therapeutic proteins (including those required for experimental purposes and clinical trials) are major products of biomanufacturing processes and considerable time and expense are expended to maximise the yield and quality of proteins produced in heterologous hosts. The production host of choice is the Gram-negative bacterium Escherichia coli for which many strains and expression systems have been developed. However,