I am a first year PhD student, working with Professor Robert Poole (University of Sheffield), Professor Jeff Green (University of Sheffield) and Dr Jamie Wood (University of York) using a systems biology approach to study respiration in Escherichia coli.
Expertise: overflow metabolism in Bacillus, mRNA, carbon catabolite regulation in Gram positive bacteria, Clostridium, carbon metabolism, regulation of gene expression, Bacillus subtilis, Molecular Biology, Microbiology
Tools: microbiology techniques, Western blot analyses, reporter gene analyses, qRT-PCR, absolute quantification, Optimal experimental design, Molecular biology techniques (RNA/DNA), northern blot analysis, cultivation, Genetic modification
Expertise: carbon catabolite regulation in Gram positive bacteria, Bacterial Cell Biology, microscopy, protein-protein interactions, functional protein expression, Bacillus subtilis, Molecular Biology
Tools: interaction analysis techniques especially SPR measurements, Cell culture, Western blot analyses, Immunofluorescence, 2-D Gel Electrphoresis, Dynamic modelling, quantitative western blot analysis, Fluorescence and confocal microscopy, Protein chemical methods (protein overproduction, Chromatography