Expertise: Genetics, Molecular Biology, Microarray analysis, Bacillus subtilis, phenotypic heterogeneity, gene regulation, stress responses, protein secretion, functional protein expression, microscopy, fluorescence protein fusions (transcriptional and translational), localisation studies
Tools: Genetic modification, Transcriptomics, Microarray analysis, Fluorecence based reporter gene analyses/single cell analyses, Site-directed mutagenesis, Fluorescence microscopy, Flow cytometry, Immunofluorescence, transposon mutagenesis, Molecular biology techniques (RNA/DNA/Protein), DNA affinity chromatography, EMSA
PhD student. Analyzing CcpA affinity to cre boxes (catabolite responsive elements) and response of B. subtilis to membrane protein overproduction stress.
Expertise: Molecular Biology, Bacillus subtilis, functional protein expression, protein-protein interactions, microscopy, Bacterial Cell Biology, carbon catabolite regulation in Gram positive bacteria
Tools: Chromatography, Fluorescence and confocal microscopy, Protein chemical methods (protein overproduction, quantitative western blot analysis, Dynamic modelling, 2-D Gel Electrphoresis, Immunofluorescence, Cell culture, Western blot analyses, interaction analysis techniques especially SPR measurements