
Robust IgM responses following intravenous vaccination with Bacille Calmette-Guerin associate with prevention of Mycobacterium tuberculosis infection in macaques. Irvine et al (2021)

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Development of an effective tuberculosis (TB) vaccine has suffered from an incomplete understanding of the correlates of protection against Mycobacterium tuberculosis (Mtb). Intravenous (i.v.) vaccination with Bacille Calmette–Guérin (BCG) provides nearly complete protection against TB in rhesus macaques, but the antibody response it elicits remains incompletely defined. Here we show that i.v. BCG drives superior antibody responses in the plasma and the lungs of rhesus macaques compared to traditional intradermal BCG administration. While i.v. BCG broadly expands antibody titers and functions, IgM titers in the plasma and lungs of immunized macaques are among the strongest markers of reduced bacterial burden. IgM was also enriched in macaques that received protective vaccination with an attenuated strain of Mtb. Finally, an Mtb-specific IgM monoclonal antibody reduced Mtb survival in vitro. Collectively, these data highlight the potential importance of IgM responses as a marker and mediator of protection against TB.
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Created: 25th Oct 2021 at 02:43
Last updated: 2nd Sep 2022 at 19:03

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Institutions: Massachusetts Institute of Technology
Researcher at BioMIcro Center, Data Management and Analysis Core; Massachusetts Institute of Technology
Projects: IMPAcTB
Web page: https://www.niaid.nih.gov/research/immune-mechanisms-protection-mycobacterium-tuberculosis
Submitter: Dikshant Pradhan
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Assays: All Metadata, Anti-Microbial Assay – Metadata, Antibody-Dependent Cellular Phagocytosis - Data Linked, Antibody-Dependent Neutrophil Phagocytosis - Data Linked, Bacterial Extraction - Metadata, DNA Extraction - Metadata, Digitally Barcoded Mtb Matrix Analysis - Data Attached, FC Receptor Binding Assay - Data Linked, Flow Cytometry - Data Linked, Flow Cytometry - Data Linked, Flow Cytometry Analysis - Data Attached, Flow Cytometry Analysis - Data Linked, Flow Cytometry Processing – Data Attached, Flow Cytometry – Data Linked, Functional Assay – Metadata, Immunohistochemistry - Data Linked, Immunohistochemistry – Data Linked, Library Creation – Metadata, Luminex Assay – Metadata, Luminex Data Processing – Data Attached, NHP Necropsy – Metadata, NHP Tissue Collection – Metadata, PET-CT Scan Analysis – Data Linked, PET-CT Scan – Data Linked, PET/CT Scan - Data Linked, Patient Visit - Metadata, Patient Visit - Metadata, Patient Visit - Metadata, Patient Visit – Metadata, Patient Visit – Metadata, Short Read Sequencing - Data Linked, Single Cell Clustering Analysis – Data Linked, Single Cell Expression Analysis - Data Linked, Single Cell Expression Matrix Analysis – Data Linked, Single Cell Sequencing Analysis – Data Linked, Single Cell Sequencing – Data Linked, Single Cell Sequencing – Data Linked, Tissue Collection - Metadata, Tissue Collection - Metadata, Tissue Collection - Metadata, Tissue Collection - Metadata, Tissue Extraction – Metadata, Titer Assay - Data Linked
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Submitter: Dikshant Pradhan
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Organisms: No organisms
SOPs: No SOPs
Data files: CEX-samples-publish2022-01-28_00-10.xlsx, NHP-samples-publish2022-01-28_00-20.xlsx
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Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
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Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Organisms: No organisms
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Assay type: Experimental Assay Type
Technology type: Technology Type
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Assay type: Experimental Assay Type
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Investigation: IMPAcTB
Organisms: No organisms
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Submitter: Dikshant Pradhan
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Assays: Luminex Assay – Metadata
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Submitter: Dikshant Pradhan
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Batch sample publishing
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Investigations: IMPAcTB
Studies: Robust IgM responses following intravenous vacc...
Assays: NHP Tissue Collection – Metadata
Creator: Dikshant Pradhan
Submitter: Dikshant Pradhan
Investigations: IMPAcTB
Studies: Robust IgM responses following intravenous vacc...
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Studies: Robust IgM responses following intravenous vacc...
Assays: Luminex Assay – Metadata
Creator: Dikshant Pradhan
Submitter: Dikshant Pradhan
Investigations: IMPAcTB
Studies: Robust IgM responses following intravenous vacc...
Assays: Luminex Assay – Metadata
Creator: Dikshant Pradhan
Submitter: Dikshant Pradhan
Investigations: IMPAcTB
Studies: Robust IgM responses following intravenous vacc...
Assays: Luminex Assay – Metadata
Creator: Dikshant Pradhan
Submitter: Dikshant Pradhan
Investigations: IMPAcTB
Studies: Robust IgM responses following intravenous vacc...
Assays: Luminex Assay – Metadata
Creator: Dikshant Pradhan
Submitter: Dikshant Pradhan
Investigations: IMPAcTB
Studies: Robust IgM responses following intravenous vacc...
Assays: Luminex Assay – Metadata
Creator: Dikshant Pradhan
Submitter: Dikshant Pradhan
Investigations: IMPAcTB
Studies: Robust IgM responses following intravenous vacc...
Assays: Luminex Assay – Metadata
Creator: Dikshant Pradhan
Submitter: Dikshant Pradhan
Investigations: IMPAcTB
Studies: Robust IgM responses following intravenous vacc...
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Investigations: IMPAcTB
Studies: Robust IgM responses following intravenous vacc...
Assays: Luminex Assay – Metadata
Creator: Dikshant Pradhan
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Investigations: IMPAcTB
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Assays: Functional Assay – Metadata
Creator: Dikshant Pradhan
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Creator: Dikshant Pradhan
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Investigations: IMPAcTB
Studies: Robust IgM responses following intravenous vacc...
Assays: Anti-Microbial Assay – Metadata
Creator: Dikshant Pradhan
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Assays: Anti-Microbial Assay – Metadata
Creator: Dikshant Pradhan
Submitter: Dikshant Pradhan
Investigations: IMPAcTB
Studies: Robust IgM responses following intravenous vacc...
Assays: Anti-Microbial Assay – Metadata
Creator: Dikshant Pradhan
Submitter: Dikshant Pradhan
Investigations: IMPAcTB
Studies: Robust IgM responses following intravenous vacc...
Assays: Luminex Assay – Metadata
Creator: Dikshant Pradhan
Submitter: Dikshant Pradhan
Investigations: IMPAcTB
Studies: Robust IgM responses following intravenous vacc...
Assays: Luminex Assay – Metadata
Creator: Dikshant Pradhan
Submitter: Dikshant Pradhan
Investigations: IMPAcTB
Creator: Dikshant Pradhan
Submitter: Dikshant Pradhan
Investigations: IMPAcTB
Studies: Robust IgM responses following intravenous vacc...
Assays: Luminex Assay – Metadata