SOPs

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284 SOPs visible to you, out of a total of 522

A method of how to measure methylglyoxal present in the growth medium.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

Measurements of K+ retained in the cytoplasm using flame photometry.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

The fluorescent DNA-binding dye used in qRT-PCR binds to all kinds of double stranded DNA. To prevent false-positive results, the RNA is treated with DNase to remove remaining DNA.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

Quantitative real-time PRC is used to compare kdpFABC expression between the E. coli strains MG1655 (wildtype)and MG1655 (kdpA4, a kdpFABC-inactive mutant after a shift to K+ limitation.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

A method to compare kdpFABC expression between MG1655 (wildtype) and MG1655 (kdpA4) after a shift to K+ limitation, the RNA was extracted from samples taken at different points in time.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

Pulsed-FRAP measure the diffusion constants in confined volumes in small cells like E. coli and other bacteria or cellular organelles.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

A procedure to measure the levels of GSH and SLG before and after exposure to MG using formic acid. A similar expreimental set up as for potassium efflux experiments is used to which a silicon oil centrifugation step is incorporated. Samples are analysed by LC-MS-MS in order to quantify intracellular GSH and SLG levels.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

The reverse transcriptase synthesizes DNA, which complements the mRNA template.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

To induce kdpFABC expression, cells are cultivated in K10 minimal medium (10mM K+) were shifted into K+ limiting growth medium (K0), containing 20 μM K+ by filtration.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

FRAP experiments are used for studying cytoplasmic diffusion in cells and cells membrane.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

Growing Escherichia coli to express KefF by adding IPTG for purification and kinetics experiments.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

Generating gene knock-ins using MJF618 expressing the defective lambdoid prophage recombination system λ red.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

Generating gene knock-outs using DY330 expressing the defective lambdoid prophage recombination system λ red.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

Experimental system that is designed to observe the in vivo stability and aggregation of a protein of interest.

Creator: Lisbeth Lyngberg

Submitter: Lisbeth Lyngberg

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