CC-TOP

In a well with a total volume of 250 µL, the following components are added: 219 µL of 0.1 M TEA buffer, pH 7.5 1 µL of L-LDH (550 U.mL-1) 10 µL of NADH (10 mg.mL-1) 20 µL of the sample to be assayed

The initial absorbance (Ainitial) was measured at 340 nm, followed by the introduction of the Li-HPA sample solution, whether diluted or not. After stabilization, the final absorbance (Afinal) was measured. The concentration of Li-HPA was determined using the equation: ...

A stock solution of BSA (Bovine serum Albumine) 1 mg/ml is made and from this solution different aliquots are picked in order to obtain different dilution solution.

1. 25 µL from solution + 975 µL water to obtain 25 µg/ml
2. 50 µL from solution + 950 µL of water to obtain 50 µg/ml
3. 75 µL from solution + 925 µL water to obtain 75 µg/ml
4. 100 µL from solution + 900 µL water to obtain 100 µg/ml
5. 125 µL from solution + 825 µL water to obtain 125 µg/ml
6. 150 µL from solution + 850 µL water to ...

Time course of the aldol addition of furfural and acetone using DERA-61 in non-optimized conditions

3D structure of DERA-61 predicted by alphafold.

3D structure of DERA-61 predicted by alphafold.

Phylogenetic tree of all the 48 metagenomic DERAs in this work, with data on the expression quality of each, and the activity towards the aldol addition of furfural/benzaldehyde against acetone/butanone/cyclobutanone.

Variation of DERA-61 melting temperature in different conditions of pH, buffer type and concentration of NaCl

Kinetic of DERA-61 towards the aldol addition of furfural and acetone.

This guidlines has been developed by Rita Volkers from Wageningen University for IBISBA project, as example to use

Poster made for NextGenBiocat conference in Heraklion (Greece)

Poster made for NextGenBiocat conference in Heraklion (Greece)

Poster realized for BIOTRANS 2023 in La Rochelle (France)

Poster for the CC-TOP organized workshop at UAM