Giuseppe Arbia

As an Innovative Training Network (ITN) , our approach to bringing about innovation is via education. The 15 Early Stage Researchers that join our project will gain multidisciplinary and cutting-edge expertise from our hosting institutions and associated partners, which is required to manipulate biological catalysts for meeting tomorrow’s demanding challenges in sustainable production processes.

CC-TOP is focused on exploiting the potential benefits of C-C bond forming enzymes (“carboligases”) ...

In a well with a total volume of 250 µL, the following components are added: 219 µL of 0.1 M TEA buffer, pH 7.5 1 µL of L-LDH (550 U.mL-1) 10 µL of NADH (10 mg.mL-1) 20 µL of the sample to be assayed

The initial absorbance (Ainitial) was measured at 340 nm, followed by the introduction of the Li-HPA sample solution, whether diluted or not. After stabilization, the final absorbance (Afinal) was measured. The concentration of Li-HPA was determined using the equation: ...

A stock solution of BSA (Bovine serum Albumine) 1 mg/ml is made and from this solution different aliquots are picked in order to obtain different dilution solution.

1. 25 µL from solution + 975 µL water to obtain 25 µg/ml
2. 50 µL from solution + 950 µL of water to obtain 50 µg/ml
3. 75 µL from solution + 925 µL water to obtain 75 µg/ml
4. 100 µL from solution + 900 µL water to obtain 100 µg/ml
5. 125 µL from solution + 825 µL water to obtain 125 µg/ml
6. 150 µL from solution + 850 µL water to ...